野生仙人掌粗多糖脱蛋白方法的研究

Study on the deproteinization method for Opuntia dillenii Haw. crude polysaccharides

摘要: 采用三氯乙酸(TCA)法、Sevag法、蛋白质等电点法对仙人掌粗多糖进行脱蛋白研究,以蛋白脱除率、多糖损失率和抗氧化性损失率为指标。结果表明:三氯乙酸法脱蛋白效果最好,浓度为3%时,蛋白脱除率为80.28%,多糖损失率为5.98%,抗氧化性损失率为34.56%。

Abstract: Trichloroacetic acid method, Sevag method and the protein isoelectric point method were used to remove protein from Opuntia dillenii Haw.crude polysaccharides.And deproteinization effects of this three methods were compared by the loss of protein, the loss of polysaccharides and the loss of inoxidizability.Results showed that with concentration of trichloroacetic acid 3%, deproteinization was most effective.In this condition, the loss of protein was 80.28%, the loss of polysaccharides was 5.98% and the loss of inoxidizability was 34.56%.