Abstract: Through different methods to deal with grape seed powder removed polyphenols, the quantity of peptides and polyphenols and its antioxidative activities was inspected.Firstly, bacillus licheniformis was cultivated to make the alkaline protease reach to a certain amount.Then grape seed powder of designated ratio was inoculated, which was the optimum process mode. The results indicated that peptides and polyphenols concentration up to the maximum 4.23g /L and 128.83mg /L.In terms of antioxidant, peptides played a key role on eliminating the hydroxyl free radicals and DPPH free radicals, and the composition of peptides might be more complicated.The clearance ability to superoxideanion free radicals should contribute to the water soluble materials of the grape seed powder, but the hydrolysate of alkaline protease also increased clearance rate about 20%.