Abstract: The change of total isoflavones content ( TIFC) during the germination of soybean had been studied through the photometric method.The results showed that the total isoflavones content in soybean increased after the germination.The germinated soybean with the bud length reached 3cm had highest TIFC.The TIFC in sprout of germinated soybean was much higher than that in raw seed and cotyledon.The isoflavone profile in cotyledon of germinated soybean was similar as in raw seed. Genistin class species was the major isoflavone in cotyledon of germinated soybean, which accounted 63% of the total amount of isoflavones.The dominated components in sprout were daidzin and malonyldaidzin, sum of them account for more than 59% of total isoflavones content.Compared to raw seed and cotyledon, higer aglycon contents in sprout was observed, among them daidzein had the highest content that accounted for 6.71% of total isoflavones content.