Abstract: Based on the single-factor experiments, the responses surface methodology (RSM) were applied to study the effect of eugenol, allylisothiocyanate, sodiumdiacetate, chitosan on the Pseudomonas spp. The result showed that the optimal concentration of ugenol, allylisothiocyanate, sodiumdiacetate, chitosan was 0.51, 0.32, 2. 24, 0. 52g / L, respectively. At this condition the bacteriostatic rate was 85. 37 %, the minimum inhibitory concentration was 2. 59g / L. And the diameter of inhibitionzone was 26. 5mm, significantly higher than the polyphenols and sodium benzoate, the inhibitory effect on the Pseudomonas spp was distinctly.