培养条件对酪酸梭菌增殖生长的影响

Effects of cultural conditions on the growth of Clostridium butyricum

摘要: 主要探讨了培养基组分、培养时间、接种量、接种温度等条件下酪酸梭菌的生长及其芽孢的成熟转化状况,以期在提高菌体浓度及芽孢率的同时降低生产成本。实验结果表明,通过优化外源性碳氮源及比例和无机盐的使用量等培养条件得到的最佳培养基配方为:1.5%玉米粉,1%豆粉,0.4%K2HPO4,0.05%MgSO4,0.02%MnSO4,同时,以6%的接种量,在95℃热接种及初始pH为7.0的条件下,培养36h可获得较高的生物量。最终使酪酸梭菌活菌浓度提高到基础培养基的4.7倍,芽孢成熟转化率的提高率约为52%。

Abstract: Effects of fermentation conditions such as medium composition, incubation time, inoculation amount and temperature.On the growth of Clostridium butyricum were investigated for improving the growth of Clostridium butyricum and reducing the cost.The conditions of carbon nitrogen ratio and the concentrations of inorganic ions were optimized.The results showed that optimized culture medium was as follows:1.5% corn flour, 1% soybean meal, 0.4% K2HPO4, 0.05% MgSO4, 0.02% MnSO4.The high biomass production of Clostridium butyricum was obtained by heat-treated inoculation at 95℃, pH7.0 and inoculation amount of 6% at 36h incubation.The productivity of viable count and the spore rate of Clostridium butyricum were finally increased by approximately 4.7- fold and 52%, respectively, under the optimal conditions.