DNS法测定山楂片中还原糖含量的研究

Study on determination of reducing sugar from Haw Flakes with DNS colorimetry method

摘要: 实验针对3,5-二硝基水杨酸（DNS）比色法测定山楂片中还原糖含量,研究了其吸收光谱及测定结果的可靠性,结果显示分析山楂片还原糖的最佳实验条件:波长为550nm、DNS显色剂用量为2.0mL、沸水浴显色时间为5min。同时,测得回归方程为y=0.656x+0.076,R2=0.9927,线性关系良好。由于该方法简便快捷,且干扰较小,较适合于常规实验室山楂片还原糖含量的测定。

Abstract: 3, 5-dinitrosalicylic acid (DNS) method was used to analyze the content of reducing sugar by spectrophotometry, and the wave-length and some relevant factors were researched in analyzing the content of haw flakes reducing sugar with DNS method.Best conditions for analyzing the content of haw flakes reducing sugar had been established as follows :wave-length was 550nm.DNS solution was 2.0mL, and duration for color development in boiling water bath was 5 minutes.Under the above conditions, the regression equation was y=0.656x+0.076, R2=0.9927.The experiment had confirmed that the DNS method was simple, fast and undisturbed.The method could be used in common laboratories for research.