响应面法优化安吉白茶抑制溶藻弧菌物质的提取工艺

Optimizing extraction conditions of anti-Vibrio alginolyticu substances from Anji white tea by response surface methodology

摘要: 考察乙醇体积分数、提取时间、超声功率对超声波法提取安吉白茶中抑制溶藻弧菌物质的影响。在单因素实验的基础上,采用三因素三水平的响应面分析法对安吉白茶抑制溶藻弧菌物质的提取工艺进行优化,依据回归分析确定最优提取条件。实验结果表明,最佳提取工艺条件为:乙醇体积分数为60%,提取时间为55min,超声功率为450W,此时的抑菌效果最好,菌落数为55CFU。

Abstract: Ultrasonic wave was applied for extraction of effective component from Anji white tea.Effects of ethanol concentration, extraction time and ultrasonic power on the anti-Vibrio alginolyticu effect of the extract were investigated.On the basis of single factor investigation, response surface methodology with three factors at three levels was adopted to optimize the extraction technology of anti-Vibrio alginolyticu substances from Anji white tea.The best extraction parameters were determined by the regression analysis.Results showed that the optimum conditions of extraction were as follows:ethanol concentration 60%, extraction time 55 minutes, ultrasonic power 450W.Under the optimized conditions, total number of bacterial colony was 55CFU.