Abstract: The LCA strain was identified as Lactobacillus pentosus according to the morphology, physiological characteristic and 16S rDNA.The fermentation products of LCA strain were analyzed by means of the chiral column HPLC.The optical purity of D-lactic acid was more than 80%.It was critical important for widening the range of application of D-lactic acid to screen suitable strain for D-lactic acid production and investigated technology of fermentation.