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中国精品科技期刊2020
应用PCR-DGGE技术研究宰后羊肉经不同处理后的微生物动态变化[J]. 食品工业科技, 2013, (11): 73-76. DOI: 10.13386/j.issn1002-0306.2013.11.073
引用本文: 应用PCR-DGGE技术研究宰后羊肉经不同处理后的微生物动态变化[J]. 食品工业科技, 2013, (11): 73-76. DOI: 10.13386/j.issn1002-0306.2013.11.073
Study on the dynamic changes of microorganisms in 2 different processing of slaughter lamb during storage by PCR-DGGE[J]. Science and Technology of Food Industry, 2013, (11): 73-76. DOI: 10.13386/j.issn1002-0306.2013.11.073
Citation: Study on the dynamic changes of microorganisms in 2 different processing of slaughter lamb during storage by PCR-DGGE[J]. Science and Technology of Food Industry, 2013, (11): 73-76. DOI: 10.13386/j.issn1002-0306.2013.11.073

应用PCR-DGGE技术研究宰后羊肉经不同处理后的微生物动态变化

Study on the dynamic changes of microorganisms in 2 different processing of slaughter lamb during storage by PCR-DGGE

  • 摘要: 应用16S rDNA变性梯度凝胶电泳(DGGE)指纹图谱和系统发育分析方法,揭示了羊屠宰后经电刺激、吊挂两种方式处理后在4℃贮藏条件下主要微生物的动态变化。DGGE图谱表明,电刺激处理组在贮藏初期具有丰富的微生物群落,但经过一段时间后,只有少数种类细菌存活并最终成为主导菌群。吊挂组较电刺激组相比细菌群落较少。DGGE优势条带经DNA序列分析表明,羊肉中的主要细菌为嗜冷杆菌、葡萄球菌、克雷伯氏菌、假单胞菌、寡养单胞菌、肠杆菌、乳酸杆菌、热死环丝菌。两个处理组在储藏初期微生物菌群差异不大,但吊挂组在第10d后以葡萄球菌为主导菌群。电刺激组在贮藏过程后期才凸显的腐败菌为假单胞菌。 

     

    Abstract: 16S rDNA DGGE (denaturing gradient gel electrophoresis) finger printing and phylogenetic analysis were used to reveal the dynamic changes of predominant bacterial in electrical stimulation and slung-load of processing for slaughter lamb during storage at 4℃. The analysis of the dynamic bacterial community directly from the DGGE profiles showed various types of bacterial communities in electrical stimulation group in the initial storage period, indicating that the contamination microorganisms were diversitied. But only few types of bacteria survived and became the predominant bacteria eventually. Compared with electrical stimulation group, the slung-load group had fewer bacterial communities. The DNA sequences of main bands were analyzed and the main bacterial of lamb was found to be Psychrobacter sp, Staphylococcus sp, Klebsiella sp, Pseudomonas sp, Stenotrophomonas sp, Enterobacter sp, Lactobacillus sp and Brochothrix thermosphacta. During the early storage, the two groups microbial flora was insignificant. But after 10d, the Staphylococcus sp. was the predominant bacteria in hanging group. The predominant bacteria of electrical stimulation group were Pseudomonas sp and Psychrobacter sp.

     

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