Abstract: Using the methods of single factor test and orthogonal test of L₉ (3～4) to study the ultrasound-assisted extraction process from Momordica charantia L.vines.The effects of proportion between water and material, ultrasound-assisted extraction temperature, extraction time and extraction pH on polysaccharide yield were studied, and ultrasound-assisted extraction method was compared with traditional extraction method.The extraction product was analyzed by ultraviolet spectrogram.The results showed that the decreasing order of importance of the four process conditions affecting polysaccharide extraction was ultrasound-assisted extraction temperature, extraction time, extraction pH and proportion of water and material.The optimial extraction conditions were extraction temperature 65℃, proportion of water and material 1:25 (g/mL) , extraction time 35min, extraction pH7 and extract twice.Under these conditions, the final polysaccharide yield was 3.08%, the efficiency of ultrasound-assisted extraction was 2.5 times that of traditional hot-water extraction.