固相萃取-超高效液相色谱法测定浓缩石榴汁中羟甲基糠醛含量

Determination of hydroxymethylfurfural in pomegranate juice concentrates by solid phase extraction-ultra performance liquid chromatography

摘要: 建立了浓缩石榴汁中羟甲基糠醛（HMF）的超高效液相色谱（UPLC）检测方法。样品用水溶解后,用BondElutENV固相萃取柱净化,UPLCHSST3色谱柱分离,乙腈-水（8+92,V/V）为流动相,检测波长285nm,外标法定量。羟甲基糠醛的检出限为0.02mg/L,在0.1～10.0mg/L的浓度范围内标准溶液的浓度与峰面积线性关系良好,在10、20、50mg/kg三个添加水平下,回收率为80.8%～110.8%,相对标准偏差（RSD）低于8.1%。该方法简便、快速、准确,可用于浓缩石榴汁中羟甲基糠醛的检测。

Abstract: An ultra performance liquid chromatography (UPLC) method had been developed for the determination of hydroxymethylfurfural (HMF) in pomegranate juice concentrate.Samples were diluted with water, and then cleaned up with a Bond Elut ENV solid phase extraction cartridges.UPLC was performed on a UPLC HSS T3 (100mm×2.1mm, 1.8μm) using acetonitrile-water (8+92, V/V) as mobile phase.The detection wavelength was 285nm.Quantitation was carried out using an external standard method.The detection limit of 5-HMF was 0.02mg/L.The results showed good linear relationship at the range of 0.1～10.0mg/L.Recoveries spiked at 10、 20、50mg/kg ranged from 80.8%to 110.8%with relative standard deviations (RSD) less than 8.1%.The method was simple, fast and accurate and could be applied to the analysis of 5-HMF content in pomegranate juice concentrate.