Abstract: Different methods were used to extract the polysaccharides from Auricularia auricular such as hot water extraction, alkali extraction, ultrasonic extraction and ultrasonic with compound enzyme.Through several purification treatment on the crude polysaccharide such as deproteinization, decoloring and DEAE -cellulose column chromatography, then the concentration, temperature and pH of the obtained purified polysaccharide were selected as the independent variable and the prothrombin time (PT) was selected as the indicator of anticoagulant experiment in vitro both in the single factor test and orthogonal test L9 (33) .The results showed that the method of ultrasonic with compound enzyme presented the highest extraction ration, which the obtained rate was 19.14%.The purity was 83.23% after being purified.Under the condition of concentration was 10%, temperature was 35℃, pH7.4 of the polysaccharides from Auricularia auricular, the prothrombin time would reach 41.8s.