米曲霉GN-1固态发酵产乳糖酶培养基的优化

Optimization of solid state fermentation medium to produce β-galactosidase by Aspergillus oryzae

摘要: 在单因素实验的基础上,采用正交实验对米曲霉GN-1产乳糖酶的固态发酵培养基成分进行了优化。结果表明,在固液比为1∶1（w/v）的固态基础培养基中添加1.5%（w/w）的硫酸铵,0.4%（w/w）的硫酸镁,3%（w/w）的蔗糖为发酵培养基,30℃发酵6d,乳糖酶活力高达156.04U/mL,是在基础培养基的条件下发酵酶活力的8.3倍。

Abstract: Based on single factor test, the solid state fermentation (SSF) medium to produce β-galactosidase by Aspergillus oryzae GN-1 were optimized by L9 (33) orthogonal test. The results showed that the β-galactosidase activity of 156.04U/mL was obtained , when SSF was carried out using wheat bran and water in 1∶1 ratio , supplemented with 1.5% (w/w) ammonium sulfate, 0.4% (w/w) magnesium sulfate and 3% (w/w) sucrose at 30℃ for 6 days, which was about 8.3 times of the β-galactosidase activity acquired on the basic medium.