Abstract: To prolong the shelf life of large yellow croaker (Pseudosciaenacrocea) , water solution of Antioxidants of Bamboo Leaves (AOB) with concentrations of 0.1% and 0.2% were applied to soak the fish samples before refrigeration at (4±1) ℃, meanwhile 0.2% of Tea Polyphenols (TP) and saline were used as positive control and blank control, respectively. During the refrigeration process, the thiobarbituric acid (TBA) value, pH, texture, the volatile basic nitrogen (TVB-N) value and the K value were determined to evaluate the effect of AOB as a biological preservative. The results showed that AOB and TP both could effectively extend the shelf life of refrigerated large yellow croaker, and AOB showed even better effect than TP under the same concentration. The best performance of AOB was observed at the concentration of 0.1%, indicating the potential of AOB applied as biological preservative of aquatic.