Abstract: The comparison on bioactivities of two sorts of extracts from the fruit body of Ganoderma lucidum obtained from supercritical CO2 fluid extraction (SFE-CO2) and ethanol extraction separately was investigated through applying a series of in vitro analytical methods including antitumor model, neurocyte anti-injury models and antioxidant model. The inhibition rate of supercritical CO2 fluid extracts (SFE-CO2E) against proliferation of tumor cell LNCaP, K562 and HepG2 was 78.37%, 87.09% and 78.85% respectively when inhibitory concentration reached 100μg/mL, which were higher than the inhibition effect of ethanol extracts (EtOHE) with 60.65%, 82.29% and 66.15% respectively. In vitro neurocyte anti-injury determination revealed that the repair effect of SFE-CO2E on cell PC12 (59.29%) was much more obvious compared with that of EtOHE (17.77%) at the concentration of 200μg/mL. The free radical scavenging experiments, however, demonstrated the scavenging activity of SFE-CO2E (29.24%, 39.43% and 17.05% respectively) was greatly lower than that of EtOHE (61.90%, 67.33% and 52.07% respectively) in analytical sequence of ·OH, H2O2 and DPPH· when effective concentration reached 800μg/mL. It was concluded that SFE-CO2 and ethanol extraction indicates various advantages on extracting different bioactive substances from the fruit body of Ganoderma lucidum.