白腐菌发酵培养及诱导剂对漆酶合成的影响

White-rot fungus fermentation cultivation and effect of inducer on laccase production

摘要: 研究了碳源、氮源及诱导剂添加方式对白腐菌P.conchatus液体培养产漆酶的影响。结果表明,当碳源为葡萄糖,氮源为酵母膏,C/N比为15∶1时,P.conchatus合成漆酶酶活达到8795U·L-1;在初始培养基中,添加1.5%麦麸与1mmol/LCu2+共同诱导漆酶合成,酶活可达到51004U·L-1;将Cu2+的添加时间改在发酵后第4d,添加量为3mmol/L,其他条件不变,漆酶酶活达到101968U·L-1,比在初始培养基中添加1mmol/LCu2+所达到的酶活高了近两倍。

Abstract: The effect of carbon source, nitrogen source and inducers on laccase production of white rot fungus P. conchatus was studied. The results showed that the laccase enzyme activity reached 8795U·L-1 when the carbon source (glucose) /nitrogen source (yeast extract) was 15∶1.1.5% wheat bran and 1mmol/L Cu2+ were added into initial medium, the enzyme activity would reach 51004U·L-1. When 3mmol/L Cu2+ was added after fermentation in the fourth day, laccase enzyme activity (101968U·L-1) was twice than the condition including 1mmol/L Cu2+ in initial medium.