响应面法优化兰州百合多糖的提取工艺

Response surface optimization of extraction of polysaccharides from Lanzhou Lily

摘要: 采用水提醇沉法对兰州百合多糖进行提取,选取提取温度、提取时间、料液比三个因素作为研究对象,以兰州百合多糖的得率为评价指标,在单因素实验的基础上,通过Box-Behnken响应面分析法优化兰州百合多糖的提取工艺条件。最终确定最佳的提取条件为:提取温度为64.7℃、提取时间为6.12h、料液比为1∶30,在此提取条件下,兰州百合多糖的理论提取率可达到12.37%。

Abstract: Polysaccharide was extracted from Lanzhou lily by water method. The influences of temperature, time and the ratio of Lily flour to solution (W/V) on the extraction rate were investigated. On the basic of single factor test, the best possible extraction parameters were obtained with the response surface methodology (RSM) , at a three-variable, three-level experiment Box-Behnken design (BBD) . The optimum extraction parameters were as follows:the temperature was 64.7℃, the time was 6.12h, the materials-liqud ratio was 1∶30. The extraction rate of polysaccharide was 12.37%.