Abstract: The lauroylsucrose synthesis under ultrasonic condition were separated and purified by the thin layer chromatography (TLC) and silica gel column chromatography, and then the purified products were analyzed by HPLC, IR, MS and NMR.The optimum conditions for TLC were confirmed:4μL of the reaction mixture were applied to silica gel G plates, developed by chloroform-methanol-aceticacid-water (V/V/V/V, 75:15:7:3) , and then detected by spraying with 10% of phosphomolybdic acid hydrate in ethanol and heated at 105℃ for 10min.The optimum conditions for silica gel column chromatography were:3g sample dissolved in eluent was applied to 35mm×700mm silica gel column (200～300mesh) and chloroform-methanol-aceticacid-water was used as mobile phase, whose flow rate was 40～50mL/h, the eluent of 1 bottle/30min was collected.The purified products were 6-O-lauroylsucrose and 6'-O-lauroylsucrose.