Abstract: According to adsorption and desorption property of macroporous resins to polyphenol, an optimal macroporous resin was chosen from ADS-7、S-8、NKA-9、NKA-Ⅱ、HPD600、AB-8、X-5、D101、D3520 resins for purifying polyphenol from Korean pine cone lamella.AB-8 was found to be the most appropriate resin.The purification of polyphenol was studied, optimum conditions were as follows:the loaded amount was 0.3BV, the concentration of polyphenol was 1.5mg/mL, the time static absorption was 3h, the volume of elution water was 3BV, the eluting solvent was 90% alcohol, the volume of alcohol was 1.6BV.Under these conditions, the purity of polyphenol was 12.51% in the purified samples, while it was 35.07% in the crude samples.In addition, the comparative study on the antioxidant ability was performed between crude and purified samples.The result showed that Fe3+ reducing power and the scavenging activity on DPPH radical were enhanced after the samples were purified.