Abstract: Based on the bibliometrics of scientific literature, CiteSpace and VOSviewer software were used to investigate exopolysaccharides (EPS) in Web of Science (WOS) core collection database and China National Knowledge Infrastructure (CNKI) database from 2013 to 2023. In the field of EPS, 4047 English literatures and 1212 Chinese literatures were analyzed by quantitative statistics and visualization. The research status and hotspots of EPS were revealed by the perspectives of annual publication numbers, authors, highly cited literatures, publication journals, publication institutions, publication countries, keyword co-occurrences and emergences. The results showed that the overall number of published papers in the field of EPS had increased year by year. Major contributors were Enes Dertli, Roberto De Philippis, Chunping Xu, etc., whereas the communication and cooperation among authors needed to be strengthened. The most highly cited literatures in the WOS and CNKI databases were Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action and Investigation of Biofilm formation Mechanism and its Influencing Factors, respectively. The top three journals were International Journal of Biological Macromolecules, Frontiers in Microbiology and Carbohydrate Polymers. The top three institutions were Centre National de la Recherche Scientifique (CNRS), Egyptian Knowledge Bank (EKB) and Chinese Academy of Sciences. The publication countries mainly were China, India and the United States, whereas academic impact of China in the field of EPS needed to be improved. Keyword co-occurrence analysis found that the hot spots were optimization of fermentation conditions, developement of EPS function and production EPS by lactic acid bacteria. It revealed that mining of biological function, biosynthesis and industrial application of EPS were the main directions of future research. Based on bibliometrics, this paper expounded and analyzed the research status and hot spots in the field of EPS, providing certain references for future research.