Abstract: Objective: Investigate the effects of Lonicera caerulea anthocyanin complex liquid preparation (compound made of Lonicera caerulea L., Polygonatum sibiricum, Inonotus obliquus, LPI) on glucose and lipid metabolism in type 2 diabetes mellitus (T2D) mice. Methods: T2D model in mice was constructed by high-fat feeding combined with streptozotocin. The mice were randomly divided into 6 groups: Blank control group, diabetes model group, low, middle and high dose sample group and metformin positive control group. After continuous intervention for 28 days, the body mass data and physiological and biochemical indexes of mice were measured and recorded, and the pathological observation of the organs of mice was performed. Results: Each dose group of LPI could increase the weight of T2D mice, improve the symptoms of organ injury, significantly reduce serum insulin level (P<0.05) and significantly increase the levels of HDL-C (P<0.05). Each dose group of LPI could significantly reduce fasting blood glucose (P<0.05) as well as insulin resistance index (P<0.05), improve glucose tolerance (P<0.05), and reduce the levels of TC, TG and LDL-C in serum of T2D mice (P<0.05) in a dose-dependent manner.