川赤芝多糖对运动疲劳状态下小鼠心肌组织的保护作用

李英基; 裴水廷; 熊鹏

doi:10.13386/j.issn1002-0306.2022050301

川赤芝多糖对运动疲劳状态下小鼠心肌组织的保护作用

Protective Effect of Ganoderma lucidum Polysaccharide on Myocardial Tissue in Mice under Exercise Fatigue

摘要

摘要: 目的：研究川赤芝多糖（Ganoderma lucidum polysaccharide，GLP）对运动疲劳小鼠心肌组织的保护作用。方法：利用沸水煎煮提取GLP，分别建立小鼠力竭游泳模型和递增负荷游泳疲劳模型，测定小鼠的力竭游泳时间、抗疲劳和抗氧化相关指标，并通过Western blot检测小鼠心肌组织中核因子E2相关因子2（NF-E2-related factor 2，Nrf2）、血红素加氧酶1（Heme oxygenase-1，HO-1）、B淋巴细胞瘤-2（B-cell lymphoma-2，Bcl-2）、Bcl-2-Associated X蛋白（Bcl-2-Associated X，Bax）、激活型半胱氨酸天冬氨酸蛋白酶3（Cysteinyl aspartate-specific proteinase-3，Cleaved Caspase-3）表达。结果：与空白对照组（Con-A1）相比，GLP组小鼠游泳时间均极显著增加（P<0.01），且随着多糖剂量提高游泳时间随之提高；与空白对照组（Con-A2）相比，GLP低（GLP-L2）、中（GLP-M2）、高（GLP-H2）剂量组小鼠肝糖原分别增加了33.55%、50.73%和66.16%（P<0.01），肌糖原分别增加了64.18%、106.80%、117.21%（P<0.01），血乳酸和血尿素氮水平分别显著降低了32.21%、42.09%、42.45%和18.40%、34.18%、34.53%；与M-Con3相比，GLP-L3、GLP-M3、GLP-H3组小鼠心肌组织中MDA含量均显著降低（P<0.05），SOD、CAT和GSH-Px均显著升高（P<0.05），通过相关性分析发现小鼠的抗疲劳指标与抗氧化酶活性指标具有较高的相关性；另外，与运动模型组（M-Con3）相比，GLP-L3、GLP-M3、GLP-H3组小鼠心肌组织中Nrf2、HO-1和Bcl-2蛋白表达量均极显著上升（P<0.01），而Bax和Cleaved Caspase-3的蛋白表达量则极显著降低（P<0.01）。结论：川赤芝多糖具有显著的抗运动疲劳作用，其对小鼠心肌组织的保护作用可能与提高心肌细胞抗氧化能力、激活Nrf2/ARE信号通路和抑制心肌细胞凋亡有关。

Abstract: Objective: To study the protective effect of Ganoderma lucidum polysaccharide (GLP) on myocardial tissue of exercise-induced fatigue mice. Method: GLP was extracted by boiling water. The exhaustive swimming model and incremental load swimming fatigue model of mice were established respectively. The exhaustive swimming time, anti-fatigue and antioxidant related indexes of mice were measured. Moreover, Western blot was used to analyze the expression of NF-E2-related factor 2 (Nrf2), Heme oxygenase-1 (HO-1), B-cell lymphoma-2 (Bcl-2), Bcl-2-Associated X (Bax) and Cysteinyl aspartate-specific proteinase-3 (Cleaved Caspase-3). Results: Compared with blank control group (Con-A1), swimming time of GLP group increased significantly (P<0.01), and swimming time increased with increase of GLP dose. Compared with blank control group (Con-A2), the liver glycogen of GLP low (GLP-L2), medium (GLP-M2) and high (GLP-H2) dose groups increased by 33.55%, 50.73% and 66.16% respectively (P<0.01), muscle glycogen increased by 64.18%, 106.80% and 117.21% respectively (P<0.01), meanwhile the levels of blood lactic acid and blood urea nitrogen decreased by 32.21%, 42.09%, 42.45% and 18.40%, 34.18%, 34.53% respectively. Compared with model group (M-Con3), MDA content in GLP-L3, GLP-M3 and GLP-H3 groups was significantly decreased (P<0.05), while SOD, CAT and GSH-Px were significantly increased (P<0.05). Correlation analysis showed that anti-fatigue indexes of mice had a high correlation with antioxidant enzyme activity indexes. In addition, compared with M-Con3, protein expression levels of Nrf2, HO-1, and Bcl-2 in GLP-L3, GLP-M3, and GLP-H3 groups were significantly increased (P<0.01), while protein expression levels of Bax and Cleaved caspase-3 were significantly decreased (P<0.01). Conclusion: GLP had significant anti-exercise fatigue effect, and its protective effect on the myocardial tissue of mice might be related to improving the antioxidant capacity of myocardial cells, activating Nrf2/ARE signaling pathway and inhibiting the apoptosis of myocardial cells.

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