Abstract:
Using
Debregeasia orientalis leaves as raw materials, the Box-Behnken model was designed by single factor experiments and the optimal technological conditions for extracting total flavonoids from
Debregeasia orientalis leaves were optimized, and the antioxidant activity
in vitro was evaluated. The results showed that the ethanol concentration, liquid-material ratio, ultrasonic time and ultrasonic temperature were 35%, 42 mL/g, 47 min and 70 ℃, respectively, which were consistent with the predicted values of Box-Behnken model. The average extraction rate of total flavonoids from
Debregeasia orientalis leaves was 3.15%±1.82%. Under these conditions, the total antioxidant capacity of extract was equivalent to 1.969 mmol/L (FeSO
4 equivalent value), and the IC
50 of DPPH free radical scavenging rate was 0.075 mg/mL, which indicated that the total flavonoids of the extract from
Debregeasia orientalis leaves could exhibit good antioxidant activity, and provide certain theoretical data support for further research and development of wild resources of
Debregeasia orientalis.