Abstract: The detection and traceability technologies of food-borne microorganisms play crucial role in food safety supervision. The traditional methods,such as biochemical identification,drug resistance analysis and serological typing,have been widely used in microbial detection and traceability analysis. With the rapid development of molecular biology,immunology,materials science,bioinformatics as well as other related disciplines and technologies,numerous simple and easy technologies have emerged with advantages of more sensitivity,more specificity and higher flux compared with the traditional ones. This paper reviews the detection and traceability technologies of food-borne microorganisms in recent years,to provide a powerful reference for inspection institutions and corresponding researchers while choosing reasonable methods.