益生菌对2型糖尿病小鼠的调节作用

白璐; 张喆; 梁曦; 吕优优; 周慧; 张峻雪; 易华西; 刘同杰; 公丕民; 冯丽荣; 冷友斌; 张兰威

doi:10.13386/j.issn1002-0306.2020.19.053

益生菌对2型糖尿病小鼠的调节作用

1. 中国海洋大学食品科学与工程学院, 山东青岛 266000;
2. 中国飞鹤有限公司, 北京 100015

基金项目:

海洋生物资源的高值化利用技术与健康功能新产品的研发(2018YFC0311201)

黑龙江省应用技术研究与开发计划重大项目母乳中活性蛋白组成、母乳渗透压及母乳中益生菌种类研究(GA16B201-2)。

详细信息

作者简介:
白璐(1995-),女,硕士研究生,研究方向:食品加工与功能食品,E-mail:bl345593568@163.com。

通讯作者:
冷友斌(1969-),男,硕士,工程师,研究方向:乳品加工,E-mail:lengyoubin@feihe.com。

张兰威(1961-),男,博士,教授,研究方向:乳品科学、食品发酵,E-mail:zhanglanwei@ouc.edu.cn

中图分类号: TS201.4
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出版历程
- 收稿日期: 2020-03-01
- 网络出版日期: 2020-11-12
- 刊出日期: 2020-09-30

Administration of Probiotics on Type 2 Diabetes Mice

1. College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China;
2. China Feihe Limited Company, Beijing 100015, China

摘要

摘要: 目的:研究益生菌对2型糖尿病小鼠的降糖效果以及潜在的降糖机制。方法:以实验室34株益生菌为研究对象,利用α-葡萄糖苷酶抑制活性筛选具有降糖功效的菌株,并通过体外表面疏水性、自聚性衡量菌株益生特性,将筛选出的目标益生菌应用于动物模型,探究其降糖功效及潜在机制。采用高脂饮食结合链脲佐菌素方法建立2型糖尿病小鼠模型,所有小鼠连续灌胃8周后,检测其血糖水平、葡萄糖耐受量、糖化血红蛋白、胰岛素水平及胰岛素抵抗状况、血清炎症因子水平、胰高血糖素样肽-1以及粪便中短链脂肪酸含量。结果:在体外筛选实验中,副干酪乳杆菌J5和干酪乳杆菌K11有着良好的抑制α-葡萄糖苷酶活性和肠道黏附能力。动物实验中,干酪乳杆菌K11能够显著降低小鼠的血糖水平(P<0.05),改善糖耐量受损以及胰岛素抵抗(P<0.05);显著降低小鼠血清中肿瘤坏死因子-α和白细胞介素-6含量;明显提高血清中胰高血糖素样肽-1水平和粪便中短链脂肪酸的含量(P<0.05)。结论:干酪乳杆菌K11可显著调节2型糖尿病小鼠的血糖,作用机制可能与其调节肠道菌群产物短链脂肪酸、促进胰高血糖素样肽-1分泌并调节炎症因子有关。
- 益生菌 /
- 2型糖尿病 /
- α-葡萄糖苷酶 /
- 血糖 /
- 胰岛素
Abstract: Objective:To study the hypoglycemic effect and mechanism of probiotics on type 2 diabetic mice. Method:The α-glucosidase inhibitory activity was used to evaluate the hypoglycemic effect of 34 strains. The hydrophobicity and self-aggregation ability were used to evaluate probiotic properties. The type 2 diabetes mouse model was established by high-fat diet combined with streptozotocin. All mice were treated with probiotics for 8 weeks,measured the blood glucose level,glucose tolerance,glycosylated hemoglobin,insulin and insulin resistance levels,serum proinflammatory cytokines levels,glucagon-like peptide-1,and the concentration of short-chain fatty acids in fecal. Result:In vitro,L. paracasei J5 and L. casei K11 showed effective inhibition of α-glucosidase,and intestinal adhesion ability. In vivo,L. casei K11 significantly reduced blood glucose level in mice(P<0.05),improved impaired glucose tolerance and insulin resistance(P<0.05). The strains significantly reduced serum tumor necrosis factor-α and interleukin-6 levels in mice. The level of glucagon-like peptide-1 in serum and the concentration of short-chain fatty acids in fecal were significantly increased(P<0.05). Conclusion:L. paracasei K11 could significantly regulate blood glucose in type 2 diabetic mice. The mechanism might be related to regulating the production of short-chain fatty acids by the gut microbiota,promoting glucagon-like peptide-1 secretion and regulating proinflammatory cytokines level.
- probiotics /
- type 2 diabetic /
- α-glucosidase /
- blood glucose /
- insulin