响应面法优化结香花总黄酮提取工艺及其抗氧化活性

张意笠; 程汝滨; 黄真; 余水生; 周樟平; 钟晓明

doi:10.13386/j.issn1002-0306.2020.19.030

响应面法优化结香花总黄酮提取工艺及其抗氧化活性

1. 浙江中医药大学药学院, 浙江杭州 310053;
2. 浙江九龙山国家级自然保护区管理局, 浙江遂昌 323300;
3. 遂昌县科技局, 浙江遂昌 323300

基金项目:

株式会社高丝研究所横向课题(2016-HT-146)

浙江中医药大学省重点建设高校优势特色学科(中药学)开放基金资助(ZYAOX2018033)。

详细信息

作者简介:
张意笠(1994-),女,硕士研究生,研究方向:中药品质评价及资源开发利用研究,E-mail:ZYL_pony@163.com。

通讯作者:
钟晓明(1962-),男,博士,教授,研究方向:中药品质评价及资源开发利用研究,E-mail:k6_zxm@sina.com。

中图分类号: TS255.1
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出版历程
- 收稿日期: 2019-11-06
- 网络出版日期: 2020-11-12
- 刊出日期: 2020-09-30

Optimization of the Extraction Technology of Total Flavonoids from Edgeworthia chrysantha Lindl.by Response Surface Analysis and Its Antioxidant Activities in Vitro

1. Zhejiang Chinese Medical University, Hangzhou 310053, China;
2. Zhejiang Jiulongshan National Nature Reserve Administration, Suichang 323300, China;
3. Technology Bureau, Suichang 323300, China

摘要

摘要: 采用响应面法优化结香花中总黄酮的提取工艺,评价其抗氧化活性。在单因素实验的基础上,以乙醇浓度、液料比、提取温度、提取时间为优化因素,总黄酮得率为评价指标,采用Box-Behnken法优化结香花总黄酮的提取工艺;利用DPPH和ABTS自由基清除实验检测结香花总黄酮的抗氧化能力。结果表明,结香花总黄酮的最佳提取工艺为乙醇浓度80%,液料比35:1,提取温度83℃,提取时间85 min,总黄酮得率22.76 mg·g^-1。最佳工艺条件下得到的提取物具有一定的清除自由基能力,且与总黄酮含量呈明显的浓度依赖性,体外清除DPPH自由基和ABTS自由基的IC₅₀值分别为0.75和0.98 mg·mL^-1。
- 结香花总黄酮 /
- 提取工艺 /
- 响应面 /
- 抗氧化活性
Abstract: The response surface method was used to optimize the extraction process of total flavonoids from Edgeworthia chrysantha Lindl and its antioxidant activity was evaluated. Based on single factor experiments,taking ethanol concentration,liquid to material ratio,extraction temperature and extraction time as optimization factors,the total flavonoid yield was an evaluation index,and the Box-Behnken method was used to optimize the extraction process of total flavonoids. The DPPH and ABTS free radical scavenging experiments were used to detect the antioxidant capacity of total flavonoids. The results showed that the optimal conditions were determined to be 80% for ethanol concentration,35:1 for liquid to material ratio,83℃for extraction temperature,85 min for extraction time,the total flavonoids yield was 22.76 mg·g^-1. The extract obtained under the optimal process conditions had a certain ability to scavenge free radicals,and had a significant concentration dependence on the total flavonoid content. The IC₅₀ values of scavenging DPPH radicals and ABTS radicals in vitro were 0.75 and 0.98 mg·mL^-1,respectively.
- total flavonoids from Edgeworthia chrysantha Lindl. /
- extraction process /
- response surface /
- antioxidant activity

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