摘要:
为了提高鸭血的高值化利用,本研究以鸭血为原料,通过单因素实验以及响应面试验确定了制备具有高亚铁螯合能力的鸭血酶解产物的最优酶解工艺,并分析了最优工艺下鸭血酶解产物的氨基酸组成和分子量分布情况。结果表明:碱性蛋白酶酶解鸭血的最佳酶解工艺为:酶添加量为9800 U/g,酶解温度50℃,pH11,酶解时间为2 h,此条件下亚铁螯合率为65.36%。相对分子质量分布结果显示,分子量在1500 Da以下的多肽组分约占72.01%。氨基酸组成分析发现酶解产物的总氨基酸含量约为76.25%,其中Lys、Glu、Asp、His含量较高,分别占氨基酸总量的10.25%、13.95%、9.67%、8.89%。这些氨基酸对亚铁离子有较强的螯合作用。优化碱性蛋白酶水解鸭血制备具有高亚铁螯合能力的鸭血酶解产物的酶解工艺可靠、合理,酶解产物具有较好的体外亚铁螯合活性。研究结果将为畜禽血液补铁剂的开发提供一定的理论依据。
Abstract:
In this study,in order to improve the high value utilization of duck blood,duck blood was used as raw material,the technological conditions for producing enzymatic hydrolysate with high iron-chelating ability of duck blood and the components of hydrolysates were studied. Based on the result of single factor experiments,response surface methodology(RSM)was carried out with the determination of iron-chelating rate. The results showed that the optimum hydrolysis conditions of duck blood by alcalase were as follows:Enzyme addition 9800 U/g,hydrolysis temperature 50℃,pH11,hydrolysis time 2 h. The actual iron-chelating rate of the peptides prepared under the optimized condition was 65.36%. The relative molecular weight of enzymatic hydrolysate was mainly below 1500 Da and accounted for 72.01%. Amino acid composition analysis showed that the contents of total amino acid were about 76.25% in the enzymatic hydrolysate,among which Lys,Glu,Asp and His were higher,accounting for 10.25%,13.95%,9.67% and 8.89% of the total amino acids,respectively. And these amino acids had stronger iron-chelating activity. The optimized process for the preparation of enzymatic hydrolysate with high iron-chelating ability from duck blood was reliable and reasonable,and hydrolysates had better iron-chelating activity in vitro. The results would provide some theoretical basis for the development of iron supplement in animal blood.
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