Abstract: In this study,in order to improve the high value utilization of duck blood,duck blood was used as raw material,the technological conditions for producing enzymatic hydrolysate with high iron-chelating ability of duck blood and the components of hydrolysates were studied. Based on the result of single factor experiments,response surface methodology(RSM)was carried out with the determination of iron-chelating rate. The results showed that the optimum hydrolysis conditions of duck blood by alcalase were as follows:Enzyme addition 9800 U/g,hydrolysis temperature 50℃,pH11,hydrolysis time 2 h. The actual iron-chelating rate of the peptides prepared under the optimized condition was 65.36%. The relative molecular weight of enzymatic hydrolysate was mainly below 1500 Da and accounted for 72.01%. Amino acid composition analysis showed that the contents of total amino acid were about 76.25% in the enzymatic hydrolysate,among which Lys,Glu,Asp and His were higher,accounting for 10.25%,13.95%,9.67% and 8.89% of the total amino acids,respectively. And these amino acids had stronger iron-chelating activity. The optimized process for the preparation of enzymatic hydrolysate with high iron-chelating ability from duck blood was reliable and reasonable,and hydrolysates had better iron-chelating activity in vitro. The results would provide some theoretical basis for the development of iron supplement in animal blood.