Abstract: The research objective was to study the optimization extraction process of Uighur medicinal tea by Box-Behnken method with total phenol as the standard using green cardamom, safflower, rose, clove, peppermint, fennel, licorice, ginger, turmeric and Fu brick tea. Optimization of the antioxidant activity and TPP from Uighur medicinal tea was carried using a single factor. The optimum extraction variables include extraction time, temperature and the ratio of solid and liquid because of the extraction parameters have a big effect on the extraction of TPP and antioxidant activity of Uighur medicinal tea. The optimal extraction conditions were determined as:extraction time 24 min, extraction temperature 85.5℃, liquid to material ratio 1:54 g/g.Under such extraction process, the yield of polyphenols was (46.86±0.814) mg/g, p=0.1723 there was no significant difference in result. The IC₅₀ of ABTS⁺and DPPH were as follows:30.837 mg/mL and 13.399 mg/mL, suggesting strong reducing power and total antioxidant capability with Uighur medicinal tea.