Simultaneous Detection of 14 Sweeteners in Pre-prepared Dishes Using High-performance Liquid Chromatography-Tandem Mass Spectrometry

WANG Xiaoyan; LI Cheng; YAN Xi; WANG Jiaxin; LIU Ziye; LIU Meiyu; TIAN Sinuo

doi:10.13386/j.issn1002-0306.2024070005

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WANG Xiaoyan, LI Cheng, YAN Xi, et al. Simultaneous Detection of 14 Sweeteners in Pre-prepared Dishes Using High-performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2025, 46(10): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070005.

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Simultaneous Detection of 14 Sweeteners in Pre-prepared Dishes Using High-performance Liquid Chromatography-Tandem Mass Spectrometry

WANG Xiaoyan^{1, 2,},
LI Cheng²,
YAN Xi^{1, 2},
WANG Jiaxin^{1, 2},
LIU Ziye^{1, 2},
LIU Meiyu^1, ,,
TIAN Sinuo^2, ,

1.
School of Life Science and Food Engineering, Hengbei University of Engineering, Handan 056000, China
2.
Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Risk Assessment Laboratory for Agro-Products (Beijing), Ministry of Agriculture and Rural Affairs, Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China

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Received Date: July 01, 2024
Available Online: March 18, 2025

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Abstract

Abstract

Given the current lack of effective methods for directly detecting sweeteners in pre-prepared dishes, this study had established an analytical method using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to simultaneously detect 14 sweeteners in pre-prepared dishes. The samples were extracted with 80% methanol-water solution (pH=3) under ultrasonic conditions for 10 minutes, followed by centrifugation at 8000 r/min for 5 minutes at 4 ℃. The extraction process was repeated once, and the combined extract was concentrated by rotary evaporation. The concentrated liquid was then diluted to 100 mL, and 5 mL of n-hexane was added. After vortexing for 2 min and purification, the sample was analyzed. The separation process was facilitated by an ACQUITY HSS T3 chromatography column (100 mm×2.1 mm, 1.8 μm), employing a gradient elution technique with two mobile phases: (A) 5 mmol ammonium acetate in 0.1% formic acid aqueous solution and (B) 5 mmol ammonium acetate in 0.1% formic acid methanol solution. For detection, the electrospray negative ion multiple reaction monitoring mode was utilized. The analysis revealed that the 14 sweeteners exhibited strong linear correlations within their respective concentration ranges, with R² values consistently exceeding 0.999. The detection limits spanned from 0.02 to 4.30 mg/kg, while quantification limits ranged between 0.07 and 14.33 mg/kg. During spiking tests conducted at concentrations of 1.0, 50.0, and 125.0 mg/kg, the recovery rates of the samples fell within the range of 86.2% to 107.1%, accompanied by relative standard deviations (RSD) of less than 10% (n=6). This method demonstrates effective purification, straightforward operation, and high sensitivity, ideal for rapid detection of 14 sweeteners in pre-prepared dishes.
- liquid chromatography-tandem mass spectrometry,
- pre-prepared dishes,
- sweeteners

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References (41)

References

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[3]	CHEN Qiuyu, ZHAO Ran, MENG Meishan, DOU Xinyu, CHEN Biyi, ZHAO Qiancheng, LI Ying. Research Progress on Immune Regulation Activities of Marine Sulfate Polysaccharides[J]. Science and Technology of Food Industry, 2025, 46(1): 413-423. DOI: 10.13386/j.issn1002-0306.2024020170
[4]	HUANG Yanyan, LIANG Yantong, WU Jiamin, ZENG Xin'an, ZENG Qiaohui, CAO Shilin, LIAO Lan, WANG Langhong. A Review of the Mechanism of Probiotics Controlling Obesity through Intestinal Flora[J]. Science and Technology of Food Industry, 2023, 44(8): 1-8. DOI: 10.13386/j.issn1002-0306.2022080280
[5]	TANG Manyu, WANG Wanqing, QIANG Jingwen, HUA Wei, WU Shuang, LI Yali, ZHEN Xin, LI Chungeng, CHENG Yanling. Interaction and Mechanism of Probiotics with Gut Flora and Immune Regulation: A Review[J]. Science and Technology of Food Industry, 2022, 43(16): 486-493. DOI: 10.13386/j.issn1002-00306.2022030025
[6]	CHEN Yanjun, LIU Jiahong, ZHANG Xiang, CUI Jingai, CHEN Xiaoping. Metabolic Regulation and Mechanism of Multi-Component Resistant Starch on High-Sugar and High-Fat Model Mice[J]. Science and Technology of Food Industry, 2021, 42(19): 357-362. DOI: 10.13386/j.issn1002-0306.2020100085
[7]	NIU Jiahui, YUAN Jing, WEI Ran, ZHANG Xiaofu, ZHANG Huifang, JIA Jinyu, ZHAO Wen. Protective Effect and Mechanism Study of Jujube Polysaccharides on Intestinal Immune Barrier in Mice[J]. Science and Technology of Food Industry, 2021, 42(4): 295-300,306. DOI: 10.13386/j.issn1002-0306.2020060068
[8]	ZHANG Xiu-juan, MA Jun-chi, ZHAO Jin-tong, GAO Shi-yong. Advances on the Effect of Berberine on Intestinal Flora[J]. Science and Technology of Food Industry, 2020, 41(23): 359-363. DOI: 10.13386/j.issn1002-0306.2020030333
[9]	XIAO Xue-jun, XINHUA·Na-bi. Research Progress on Immunomodulation and Antitumor Effect of Probiotics[J]. Science and Technology of Food Industry, 2020, 41(10): 321-326. DOI: 10.13386/j.issn1002-0306.2020.10.054
[10]	DU Xiao-lin, HUANG Huang, LI Qian, WU Zheng-qi, CHEN Xiao-qiang, MI Zhi-yuan. Advances in anti-tumor effect of catechins EGCG and its mechanism[J]. Science and Technology of Food Industry, 2017, (24): 308-314. DOI: 10.13386/j.issn1002-0306.2017.24.060