Optimization of Protease Digestion Process of Tuna Cooking Liquid Based on Bone-enhancing Activity

WU Yuxi; WANG Ying; LIN Xiaozi; CHEN Bingyan; HE Zhigang

doi:10.13386/j.issn1002-0306.2024060372

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WU Yuxi, WANG Ying, LIN Xiaozi, et al. Optimization of Protease Digestion Process of Tuna Cooking Liquid Based on Bone-enhancing Activity[J]. Science and Technology of Food Industry, 2025, 46(11): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060372.

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Optimization of Protease Digestion Process of Tuna Cooking Liquid Based on Bone-enhancing Activity

WU Yuxi^{1, 2,},
WANG Ying^{1, 2},
LIN Xiaozi^{1, 2},
CHEN Bingyan^{1, 2, ,},
HE Zhigang^{1, 2, ,}

1.
Agricultural Product Processing Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
2.
Key Laboratory of Agricultural Products (Food) Processing, Fuzhou 350003, China)

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Received Date: June 26, 2024
Available Online: March 27, 2025

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Abstract

In order to clarify the physicochemical characteristics of the proteolytic digest of tuna cooking liquid and to investigate its osteoblast-promoting activity. The proteins in the tuna cooking liquor were separately hydrolysed by six proteases. The effects of different proteases on the proteolysis, molecular weight and amino acid composition of cooking fluids were investigated. The osteoblast-proliferative activities of different proteolyte was evaluated and the enzymatic process was optimized. Trypsin showed the highest proteolysis (53.31%) of tuna cooking liquid protein. The proportion of small molecule peptides in the hydrolysate was 85.85% and was rich in glycine, tyrosine, phenylalanine that promote osteoblast proliferation. Trypsin hydrolysate had the best osteoblast-proliferative activity, the dose-response relationship was consistent with y=0.4139x³−20.558x²+49.581x+99.203 (R²=0.9597). The optimal enzymatic process of trypsin was 3.5 h, 50 ℃, 10000 U/g specific activity of enzyme and pH8.0. Under the optimal process, the osteoblast proliferation rate of the tuna cooking liquid protein hydrolysate was 136.76%. Thus, trypsin has the potential to produce active peptides for osteoblast proliferation in tuna.
- tuna cooking liquid,
- osteoblast proliferation peptides,
- hydrolysate,
- process optimization

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References

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