Optimization of Preparation Technology and <i>in Vitro</i> Digestion Analysis of Hydrolysates Origin from by-Products of Mandarin Fish

WU Zhanchun; LI Ling; ZHOU Yingqin; XIE Ningning; FANG Xubo; CHEN Xiaoe

doi:10.13386/j.issn1002-0306.2022010184

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 195-202. > DOI: 10.13386/j.issn1002-0306.2022010184

WU Zhanchun, LI Ling, ZHOU Yingqin, et al. Optimization of Preparation Technology and in Vitro Digestion Analysis of Hydrolysates Origin from by-Products of Mandarin Fish[J]. Science and Technology of Food Industry, 2022, 43(21): 195−202. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010184.

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Optimization of Preparation Technology and in Vitro Digestion Analysis of Hydrolysates Origin from by-Products of Mandarin Fish

WU Zhanchun^{1, 2,},
LI Ling^{1, 2},
ZHOU Yingqin^{2, 3},
XIE Ningning^{2, 3, ,},
FANG Xubo⁴,
CHEN Xiaoe^1, ,

1.
School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
2.
Institute of Agricultural Products Processing, Anhui Academy of Agricultural Sciences, Hefei 230031, China
3.
Anhui Food Microbial Fermentation and Functional Application Engineering Laboratory, Hefei 230041, China
4.
Zhejiang International Maritime Vocational and Technical College, Zhoushan 316021, China

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Received Date: January 20, 2022
Available Online: August 31, 2022

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Abstract

Abstract

The by-products of mandarin fish were hydrolyzed with alcalase, while its optimized preparing process was performed with the single factor and response surface experiments, which were based on the degree of hydrolysis. Additionally, the in vitro digestion of prepared hydrolysates was further analyzed. The results showed that: The optimum condition of the hydrolysis processing was that the ratio of solid-liquid was 1:4 g/mL, the pH was 9.1, the temperature was 57.0 ℃, the enzyme dosage was 2.02%, and the hydrolysis time was 4 h, with the degree of hydrolysis of which was 18.38%. During the simulated gastric digestion and the simulated intestinal digestion, the content of soluble peptides respectively decreased by 17.76% and 50.97%. Furthermore, after the whole gastrointestinal digestion, DPPH and ABTS radical scavenging rate, respectively, survived with values of 51.36% and 48.67%, which indicated that the prepared hydrolysates exerted relatively higher bioaccessibility. The results suggest that the by-products of mandarin fish might be utilized as a candidate for developing functional foods.
- mandarin fish,
- by-products,
- hydrolysates,
- antioxidant activity,
- in vitro digestion

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References

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