WANG Ruihong, ZHANG Kunlin, YANG Panchuang, et al. Frozen Egg Yolk Gelation Mechanism and Inhibition Method: A Review[J]. Science and Technology of Food Industry, 2025, 46(9): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060206.
Citation: WANG Ruihong, ZHANG Kunlin, YANG Panchuang, et al. Frozen Egg Yolk Gelation Mechanism and Inhibition Method: A Review[J]. Science and Technology of Food Industry, 2025, 46(9): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060206.

Frozen Egg Yolk Gelation Mechanism and Inhibition Method: A Review

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  • Received Date: June 13, 2024
  • Available Online: February 28, 2025
  • The egg yolk is rich in nutrients and is a widely used ingredient in processed foods owing to its excellent emulsifying properties. Freezing can maximize the preservation of the original flavor of the egg yolk, inhibit microbial growth, minimize quality decline during transportation and storage, and extend its shelf life. However, upon thawing, the fluidity of the frozen yolk decreases, viscosity increases, and gelation occurs. Gelation makes it difficult to mix the yolk with other food ingredients, thereby reducing its dispersibility and functionality. To better understand the phenomenon of frozen yolk gelation, herein, this paper reviews up-to-date research on the mechanisms through which frozen yolk gelation occurs, including the formation of ice crystals, lipoprotein aggregation during freezing, and the influence of environmental factors. Various methods to inhibit frozen yolk gelation are also explored, including physical, chemical, and enzymatic methods. Because different methods inhibit frozen yolk gelation through different mechanisms, understanding the causes of frozen yolk gelation and the mechanisms underlying the methods of its inhibition can provide theoretical bases for improving the useability of frozen yolks in food preparation.
  • [1]
    迟玉杰. 蛋制品加工技术[M]. 中国轻工业出版社, 2009. [CHI Y J. Egg processing technology[M]. China Light Industry Press Co., Ltd., 2009.]

    CHI Y J. Egg processing technology[M]. China Light Industry Press Co., Ltd., 2009.
    [2]
    周建军, 于爱芝, 王长梅. 我国鸡蛋价格波动传导的影响因素分析——基于非对称的研究视角[J]. 中国农业资源与区划,2022,44:1−15. [ZHOU J J, YU A Z, WANG C M. Analysis of factors influencing the transmission of egg price fluctuations in China:Based on asymmetric research perspective[J]. Chinese Journal of Agricultural Resources and Regional Planning,2022,44:1−15.]

    ZHOU J J, YU A Z, WANG C M. Analysis of factors influencing the transmission of egg price fluctuations in China: Based on asymmetric research perspective[J]. Chinese Journal of Agricultural Resources and Regional Planning, 2022, 44: 1−15.
    [3]
    VIEIRA D A P, CABRAL L, NORONHA M F, et al. Microbiota of eggs revealed by 16S rRNA-based sequencing:From raw materials produced by different suppliers to chilled pasteurized liquid products[J]. Food Control,2019,96:194−204. doi: 10.1016/j.foodcont.2018.09.016
    [4]
    MA Z, MA Y, WANG R, et al. Influence of antigelation agents on frozen egg yolk gelation[J]. Journal of Food Engineering,2021,302:110585. doi: 10.1016/j.jfoodeng.2021.110585
    [5]
    LI T, ZHONG Q, WU T. Effects of NaCl on the freezing-thawing induced gelation of egg yolk at pH 2.0-8.0[J]. Food Biophysics,2022,17:106−113.
    [6]
    PRIMACELLA M, ACEVEDO N C, WANG T. Effect of freezing and food additives on the rheological properties of egg yolk[J]. Food Hydrocolloids,2020,98:105241. doi: 10.1016/j.foodhyd.2019.105241
    [7]
    HARRISON L J, CUNNINGHAM F E. Influence of frozen storage time on properties of salted yolk and its functionality in mayonnaise[J]. Journal of Food Quality,2010,9(3):167−174.
    [8]
    PRIMACELLA M, FEI T, ACEVEDO N, et al. Effect of food additives on egg yolk gelation induced by freezing[J]. Food Chemistry,2018,263:142−150. doi: 10.1016/j.foodchem.2018.04.071
    [9]
    闫峥蓉, 赵英, 迟玉杰. 冻藏期间蛋黄胶凝化现象及成因分析[J]. 食品科学,2018,39(19):29−35. [YAN Z R, ZHAO Y, CHI Y J. Origin analysis for freezing-thawing gelation of yolk appears during frozen storage[J]. Food Science,2018,39(19):29−35.] doi: 10.7506/spkx1002-6630-201819006

    YAN Z R, ZHAO Y, CHI Y J. Origin analysis for freezing-thawing gelation of yolk appears during frozen storage[J]. Food Science, 2018, 39(19): 29−35. doi: 10.7506/spkx1002-6630-201819006
    [10]
    WANG R, MA Y, ZHANG L, et al. Changes in egg yolk gelation behaviour and mechanisms during freezing[J]. LWT,2021,151:112223. doi: 10.1016/j.lwt.2021.112223
    [11]
    HARRISON L J, CUNNINGHAM F E. Factors influencing the quality of mayonnaise[J]. Journal of Food Quality,2010,8(1):1−20.
    [12]
    PRIMACELLA M, WANG T, ACEVEDO N C. Characterization of mayonnaise properties prepared using frozen-thawed egg yolk treated with hydrolyzed egg yolk proteins as anti-gelator[J]. Food Hydrocolloids,2019,96:529−536. doi: 10.1016/j.foodhyd.2019.06.008
    [13]
    HUANG L, WANG T, HAN Z, et al. Effect of egg yolk freezing on properties of mayonnaise[J]. Food Hydrocolloids,2016,56:311−317. doi: 10.1016/j.foodhyd.2015.12.027
    [14]
    GMACH O, GOLDA J, KULOZIK U. Freeze-thaw stability of emulsions made with native and enzymatically modified egg yolk fractions[J]. Food Hydrocolloids,2022,123:107109. doi: 10.1016/j.foodhyd.2021.107109
    [15]
    LU F, CHI Y, CHI Y. High-temperature glycosylation of saccharides to modify molecular conformation of egg white protein and its effect on the stability of high internal phase emulsions[J]. Food Research International,2024,176:113825. doi: 10.1016/j.foodres.2023.113825
    [16]
    ZHU Z, ZHOU Q, SUN D W. Measuring and controlling ice crystallization in frozen foods:A review of recent developments[J]. Trends in Food Science & Technology,2019,90:13−25.
    [17]
    ZHAO Y, FENG F, YANG Y, et al. Gelation behavior of egg yolk under physical and chemical induction:A review[J]. Food Chemistry,2021,355(5):129569.
    [18]
    LUAN L, WANG L, WU T, et al. A study of ice crystal development in hairtail samples during different freezing processes by cryosectioning versus cryosubstitution method[J]. International Journal of Refrigeration,2018,87:39−46. doi: 10.1016/j.ijrefrig.2017.10.014
    [19]
    LI F, DU X, WANG B, et al. Inhibiting effect of ice structuring protein on the decreased gelling properties of protein from quick-frozen pork patty subjected to frozen storage[J]. Food Chemistry,2021,353:129104. doi: 10.1016/j.foodchem.2021.129104
    [20]
    LIU L, WANG J, WANG G, et al. Quantitative proteomics provides a new perspective on the mechanism of network structure depolymerization during egg white thinning[J]. Food Chemistry,2022,392:133320. doi: 10.1016/j.foodchem.2022.133320
    [21]
    DENG L, WANG Y, JIANG H, et al. Specific protection mechanism of oligosaccharides on liposomes during freeze-drying[J]. Food Research International,2023,166:112608. doi: 10.1016/j.foodres.2023.112608
    [22]
    WANG T, AU C, ACEVEDO N, et al. Determination of the gelation mechanism of freeze-thawed hen egg yolk[J]. Journal of Agricultural & Food Chemistry,2015,63(46):10170−10180.
    [23]
    VRN T, KIECKBUSCH T G. Viscoelasticity of frozen/thawed egg yolk[J]. Journal of Food Science,2010,62(3):548−550.
    [24]
    MA Z, CHI Y, CHI Y. Cryoprotective role of saccharides in frozen egg yolks:Water/ice tailoring effect and improved freeze–thaw stability[J]. Food Hydrocolloids,2023,145:109161. doi: 10.1016/j.foodhyd.2023.109161
    [25]
    CHEN Q, XIE Y, YU H, et al. Non-destructive prediction of colour and water-related properties of frozen/thawed beef meat by Raman spectroscopy coupled multivariate calibration[J]. Food Chemistry,2023,413:135513. doi: 10.1016/j.foodchem.2023.135513
    [26]
    PRIMACELLA, MONICA. Determination of gelation mechanism and prevention methods of frozen-thawed hen egg yolk[J]. Journal of Agricultural and Food Chemistry,2017,46:1−88.
    [27]
    WANG X, LI J, SU Y, et al. Freeze-thaw treatment assists isolation of IgY from chicken egg yolk[J]. Food Chemistry,2021,364:130225. doi: 10.1016/j.foodchem.2021.130225
    [28]
    KURISAKI J I, KAMINOGAWA S, YAMAUCHI K. Studies on freeze-thaw celation of very low density lipoprotein from hen’s egg yolk[J]. Journal of Food Science,1980,45:463−466. doi: 10.1111/j.1365-2621.1980.tb04076.x
    [29]
    KAMAT V, GRAHAM G, BARRATT M, et al. Freeze-thaw gelation of hen’s egg yolk low density lipoprotein[J]. Journal of the Science of Food and Agriculture,1976,27:913−927. doi: 10.1002/jsfa.2740271005
    [30]
    WANG R, ZHANG L, CHI Y, et al. Forces involved in freeze-induced egg yolk gelation:Effects of various bond dissociation reagents on gel properties and protein structure changes[J]. Food Chemistry,2022,371:131190. doi: 10.1016/j.foodchem.2021.131190
    [31]
    WANG R, MA Y, MA Z, et al. Changes in gelation, aggregation and intermolecular forces in frozen-thawed egg yolks during freezing[J]. Food Hydrocolloids,2020,108:105947. doi: 10.1016/j.foodhyd.2020.105947
    [32]
    FEI T, LEYVA-GUTIERREZ F M A, WAN Z, et al. Gelation inhibiting additives and freezing impact rheological, thermal, and microstructural properties of yolk[J]. LWT,2021,144:111160. doi: 10.1016/j.lwt.2021.111160
    [33]
    WAKAMATU T, SATO Y, SAITO Y. On sodium chloride action in the gelation process of low density lipoprotein (LDL) from hen egg yolk[J]. Journal of Food Science,2010,48(2):507−512.
    [34]
    HUANG L, WANG T, HAN Z, et al. Effect of egg yolk freezing on properties of mayonnaise[J]. Food Hydrocolloids,2016,56(5):311−317.
    [35]
    POWRIE W D, LITTLE H, LOPEZ A J. Gelation of egg yolk[J]. Journal of Food Science,1963,28(1):38−46. doi: 10.1111/j.1365-2621.1963.tb00156.x
    [36]
    LOPEZ A, FELLERS C R, POWRIE W D. Some factors affecting gelation of frozen egg yolk[J]. Journal of Milk Food Technology,1954,17(11):1−10.
    [37]
    杜清普, 赵英, 王瑞红, 等. 不同解冻方法对冰蛋黄功能特性, 理化特性及蛋白质结构的影响[J]. 食品科学,2021,42(11):9−15. [DU Q P, ZHAO Y, WANG R H, et al. Effects of different thawing method on the functional properties, physicochemical properties, and protein structures of frozen egg yolks[J]. Food Science,2021,42(11):9−15.]

    DU Q P, ZHAO Y, WANG R H, et al. Effects of different thawing method on the functional properties, physicochemical properties, and protein structures of frozen egg yolks[J]. Food Science, 2021, 42(11): 9−15.
    [38]
    ZHAO Y, FENG F, YANG Y, et al. Gelation behavior of egg yolk under physical and chemical induction:A review[J]. Food Chemistry,2021,355:129569. doi: 10.1016/j.foodchem.2021.129569
    [39]
    KUMAR S A, MAHADEVAN S J J O A. Physicochemical studies on the gelation of hen's egg yolk:Delipidation of yolk plasma by treatment with phospholipase-C and extraction with solvents[J]. Journal of Agricultural Food Chemistry,1970,18(4):666−670. doi: 10.1021/jf60170a014
    [40]
    JAAX S, TRAVNICEK D. The Effect of pasteurization, selected additives and freezing rate on the gelation of frozen-defrosted egg yolk[J]. Poultry Science,1968,47(3):1013−1022. doi: 10.3382/ps.0471013
    [41]
    PEARCE J A, LAVERS C G. Liquid and frozen egg; viscosity, baking quality, and other measurements on frozen egg products[J]. Canadian Journal of Research,1949,27(5):231−240.
    [42]
    MINE Y. Egg bioscience and biotechnology[J]. Nano Today,2008,3(5):48.
    [43]
    ZHANG J, MA Y. Formation mechanism and inhibition methods of frozen egg yolk gelation:A review[J]. Trends in Food Science & Technology,2024,148:104491.
    [44]
    HADIPERNATA M, OGAWA M, HAYAKAWA S. Improved rheological properties of chicken egg frozen gels fortified by ᴅ-ketohexoses:H adipernata et al[J]. Journal of Food Processing & Preservation,2017,41(5):13184−13194.
    [45]
    URBAN O M, MILLER J N. Relative merits of sucrose, dextrose, and levulose as used in the preservation of eggs by freezing[J]. Indengchem,1930,22:355−356.
    [46]
    XU X, LI Z, TANG Q, et al. Exploring xylitol as a low-salt alternative for effective inhibition of gelation in frozen egg yolks[J]. Food Chemistry,2024,436:137681. doi: 10.1016/j.foodchem.2023.137681
    [47]
    ZHU H, LI J, SU Y, et al. Sugar alcohols as cryoprotectants of egg yolk:Inhibiting crystals and interactions[J]. Journal of Food Engineering,2023,342:111360. doi: 10.1016/j.jfoodeng.2022.111360
    [48]
    MA Z, QING M, ZANG J, et al. Effects of freezing on the gelation behaviors of liquid egg yolks affected by saccharides:Thermal behaviors and rheological and structural changes[J]. Poultry Science,2024,103(6):103657. doi: 10.1016/j.psj.2024.103657
    [49]
    LI X, HUANG Q, ZHANG Y, et al. Inhibitory mechanism of trypsin combined with d-galactose on freeze-induced gelation and the physicochemical and protein properties of egg yolk[J]. LWT,2023,188:115337. doi: 10.1016/j.lwt.2023.115337
    [50]
    YE H, SUI J, WANG J, et al. Research note:Aggregation-depolymerization of chicken egg yolk granule under different food processing conditions[J]. Poultry Science,2023,102(7):102696. doi: 10.1016/j.psj.2023.102696
    [51]
    DADASHI S, KIANI H, RAHIMI H, et al. Effect of freezing-thawing and stabilizers on the phase behavior of egg micro-particles and quality attributes of liquid egg[J]. Journal of Agricultural Science,2017,19(4):821−834.
    [52]
    SU Y, CHEN Z, LI J, et al. Characterization of salted egg yolk flavoring prepared by enzyme hydrolysis and microwave irradiation[J]. Food Chemistry,2021,338:127913. doi: 10.1016/j.foodchem.2020.127913
    [53]
    TANG C, ZHOU K, ZHU Y, et al. Collagen and its derivatives:From structure and properties to their applications in food industry[J]. Food Hydrocolloids,2022,131:107748. doi: 10.1016/j.foodhyd.2022.107748
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