Hypoglycemic Effect of <i>Moringa oleifera</i> Leaf Polysaccharide on STZ-induced Diabetic Mice and Its Mechanism

ZHANG Shiqi; WANG Rui; LI Chengliang; LI Jiaqian; TAO Hongli; DENG Yujie; ZHANG Weiguo

doi:10.13386/j.issn1002-0306.2024040369

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 357-365. > DOI: 10.13386/j.issn1002-0306.2024040369

ZHANG Shiqi, WANG Rui, LI Chengliang, et al. Hypoglycemic Effect of Moringa oleifera Leaf Polysaccharide on STZ-induced Diabetic Mice and Its Mechanism[J]. Science and Technology of Food Industry, 2024, 45(19): 357−365. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040369.

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Hypoglycemic Effect of Moringa oleifera Leaf Polysaccharide on STZ-induced Diabetic Mice and Its Mechanism

1.
College of Laboratory of Food Science and Engineering, Lingnan Normal University,Zhanjiang 524048, China
2.
Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing University of Education, Chongqing 400067, China
3.
School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, China

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Received Date: April 23, 2024
Available Online: August 19, 2024

Graphical Abstract

Abstract

Abstract

To explore the hypoglycemic effect of Moringa oleifera leaf polysaccharide (MOLP) on diabetic mice and its corresponding mechanism, a streptozotocin (STZ)-induced diabetic mouse was modeled. The mice were then divided into groups exposed to low (100 mg/kg·bw), medium (200 mg/kg·bw), and high dosage (400 mg/kg·bw) of MOLP, with a normal blank group, model group and a positive drug group (hydrochloric acid dimethylbiguanide, 200 mg/kg·bw). Eight mice in each group were gavaged for 28 d. Fasting blood glucose, serum glycated protein, serum insulin, hepatic/myocardial glycogen and other biochemical indexes were determined. Additionally, the key genes of glucose metabolism, including liver X receptor (LXR), pancreatic-duodenal homeobox-1 (PDX-1), glucokinase (GK), phosphoenolpyruvate carboxykinase (PEPCK), glucose 6-phosphatase (G6Pase), glucose transporter type 2 (GLUT2) and insulin receptor substrate 1/2 (IRS1/2) were measured in the liver and the pancreas. Furthermore, the mice's liver and pancreas tissues in each group were stained with HE to observe their histomorphology. The results showed that MOLP exhibited a significant hypoglycemic effect and a dose-response relationship. The level of blood glucose reduction in the MOLP high-dose group (400 mg/kg·bw) was closest to that of the positive drug group. The underlying mechanism was that the significantly upregulated expression of LXR and PDX-1 ameliorated the glucose metabolism disorders in diabetic mice by regulating the expression of their downstream genes including PEPCK, G6Pase, GK, GLUT2 and IRS1/2 mRNA. This regulation facilitated the improvement of the damaged liver and pancreatic tissues, increased serum insulin and hepatic glycogen content and ultimately resulted in a hypoglycemic effect.
- Moringa oleifera leaf polysaccharide,
- STZ induced mice,
- lowering blood sugar,
- glucose metabolism,
- insulin

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References

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