Improvement in the Glycolipid Metabolism of Diabetic Mice Induced by High-fat Diet with Streptozotocin Supplemented with <i>Ganoderma lucidum</i> Polysaccharide Beverage

LI Jiaxue; LI Cheng; SONG Xiaobing; CHEN Yanjun; YE Shiyi; ZHANG Biying; CHEN Ping

doi:10.13386/j.issn1002-0306.2023110302

Volume 45 Issue 19

Sep. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(19): 9-16. > DOI: 10.13386/j.issn1002-0306.2023110302

LI Jiaxue, LI Cheng, SONG Xiaobing, et al. Improvement in the Glycolipid Metabolism of Diabetic Mice Induced by High-fat Diet with Streptozotocin Supplemented with Ganoderma lucidum Polysaccharide Beverage[J]. Science and Technology of Food Industry, 2024, 45(19): 9−16. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110302.

Citation:

PDF (2510 KB)

Improvement in the Glycolipid Metabolism of Diabetic Mice Induced by High-fat Diet with Streptozotocin Supplemented with Ganoderma lucidum Polysaccharide Beverage

1.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130000, China
2.
Jilin Agricultural Science and Technology University, Changchun 130000, China
3.
Jilin Zhongke Special Food Innovation Research Institute Corporation, Changchun 130000, China

More Information

Received Date: November 29, 2023
Available Online: August 01, 2024

Graphical Abstract

Abstract

Abstract

This study aimed to investigate the effects of Changbaishan Ganoderma lucidum seed body polysaccharide beverage on the glucose and lipid metabolism of diabetic mice. High-fat diet combined with intraperitoneal injection of streptozotocin was used to establish a diabetic mouse model. A normal control group and a model control group were also set up. Metformin hydrochloride was used as a positive control. G. lucidum polysaccharide beverage was administered at different doses of low (L-GLB), medium (M-GLB), and high (H-GLB) to investigate its improvement effects on the glucose and lipid metabolism of diabetic mice. Fasting blood glucose, insulin resistance index, triglyceride, high- and low-density lipoprotein cholesterol, superoxide dismutase activity, and malondialdehyde were measured in the mice after receiving the G. lucidum polysaccharide beverage. Compared with those of the model group, the fasting blood glucose levels of the mice in the L-GLB, M-GLB, and H-GLB groups decreased by 35.46%, 39.05%, and 42.28%, respectively, and their homeostatic model assessment of insulin resistance decreased by 43.10%, 50.25%, and 54.18%, respectively (P<0.01). The triglyceride levels and LDL cholesterol of the mice in the high-dose group decreased by 46.83% and 50.79%, respectively, and their HDL cholesterol increased by 107.81%. G. lucidum polysaccharide beverage improved the superoxide dismutase activity in the serum of diabetic mice and significantly reduced the malondialdehyde content (P<0.01) by 18.66%, 30.15%, and 44.54% in the L-GLB, M-GLB, and H-GLB groups, respectively. Therefore, G. lucidum polysaccharide beverage can relieve diabetes-induced oxidative stress and organ enlargement, protect the liver, kidney and spleen of diabetic mice, and improve the liver damage caused by long-term hyperglycemia to a certain extent.
- Ganoderma lucidum polysaccharide beverage,
- diabetes model,
- glucolipid metabolism,
- mouse experiment

FullText(HTML)

References (31)

References

[1]	MA Quantao, LI Yaqi, LI Pengfei, et al. Research progress in the relationship between type 2 diabetes mellitus and intestinal flora[J]. Biomedicine & Pharmacotherapy,2019,117:109−138.
[2]	马永强, 张凯, 王鑫, 等. 甜玉米芯多糖对糖尿病大鼠的降血糖作用[J]. 食品科学,2020,41(13):169−173. [MA Yongqiang, ZHANG Kai, WANG Xin, et al. Hypoglycaemic effects of sweet corn kernel polysaccharides in diabetic rats[J]. Food Science,2020,41(13):169−173.] doi: 10.7506/spkx1002-6630-20190702-030 MA Yongqiang, ZHANG Kai, WANG Xin, et al. Hypoglycaemic effects of sweet corn kernel polysaccharides in diabetic rats[J]. Food Science, 2020, 41（13）: 169−173. doi: 10.7506/spkx1002-6630-20190702-030
[3]	HAN Xiao, YANG Fei, ZHANG Zhengyi, et al. 4EBP2-regulated protein translation has a critical role in high-fat diet–induced insulin resistance in hepatocytes[J]. Journal of Biological Chemistry,2023,299(11):105315−105315. doi: 10.1016/j.jbc.2023.105315
[4]	刘芬芬, 蒲首丞, 赵雯靓, 等. 金花茶花对2型糖尿病小鼠的降糖及抗氧化作用[J]. 食品科学, 2024, 45(3): 94-101. [LIU Fenfen, PU Shousheng, ZHAO Wenliang, et al. Hypoglycaemic and antioxidant effects of Camellia sinensis on type 2 diabetic mice[J]. Food Science, 2024, 45(3): 94-101.] LIU Fenfen, PU Shousheng, ZHAO Wenliang, et al. Hypoglycaemic and antioxidant effects of Camellia sinensis on type 2 diabetic mice[J]. Food Science, 2024, 45(3): 94-101.
[5]	LI Jia, GU Feifei, CAI Chao, et al. Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum[J]. International Journal of Biological Macromolecules,2020,143(C):806−813.
[6]	MARCELA R D C, TALITA F F, KELLY K I, et al. Ganoderma lucidum polysaccharides inhibit in vitro tumorigenesis, cancer stem cell properties and epithelial-mesenchymal transition in oral squamous cell carcinoma[J]. Journal of Ethnopharmacology,2021,286(25):114891−114905.
[7]	吴睿婷, 付王威, 万敏, 等. 黑灵芝多糖对糖尿病大鼠血糖血脂调节及肠道菌群的影响[J]. 食品科学,2022,43(5):91−102. [WU Ruiting, FU Wangwei, WAN Min, et al. Effects of black Ganoderma lucidum polysaccharides on the regulation of blood glucose, blood lipids and intestinal flora in diabetic rats[J]. Food Science,2022,43(5):91−102.] doi: 10.7506/spkx1002-6630-20201203-046 WU Ruiting, FU Wangwei, WAN Min, et al. Effects of black Ganoderma lucidum polysaccharides on the regulation of blood glucose, blood lipids and intestinal flora in diabetic rats[J]. Food Science, 2022, 43（5）: 91−102. doi: 10.7506/spkx1002-6630-20201203-046
[8]	KANG Qiaozhen, CHEN Sisi, LI Shufang, et al. Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction[J]. International Journal of Biological Macromolecules,2018,124(C):1137−1144.
[9]	LI Lu, XU Jiaxin, CAO Yingjia, et al. Preparation of Ganoderma lucidum polysaccharide chromium (III) complex and its hypoglycemic and hypolipidemic activities in high-fat and high-fructose diet-induced pre-diabetic mice[J]. International Journal of Biological Macromolecules,2019,140:782−793. doi: 10.1016/j.ijbiomac.2019.08.072
[10]	REN Lei. Protective effect of ganoderic acid against the streptozotocin induced diabetes, inflammation, hyperlipidemia and microbiota imbalance in diabetic rats[J]. Saudi Journal of Biological Sciences,2019,26(8):1961−1972. doi: 10.1016/j.sjbs.2019.07.005
[11]	陈彦君, 刘家宏, 张翔, 等. 多元复合抗性淀粉对高糖高脂模型小鼠代谢调节作用及机制[J]. 食品工业科技,2021,42(19):357−362. [CHEN Yanjun, LIU Jiahong, ZHANG Xiang, et al. Metabolic regulation and mechanism of multivariate composite resistant starch in high glucose and high fat model mice[J]. Food Industry Science and Technology,2021,42(19):357−362.] CHEN Yanjun, LIU Jiahong, ZHANG Xiang, et al. Metabolic regulation and mechanism of multivariate composite resistant starch in high glucose and high fat model mice[J]. Food Industry Science and Technology, 2021, 42（19）: 357−362.
[12]	丁霄霄. 灵芝活性成分的制备及其残渣利用[D]. 镇江:江苏大学, 2019:22-27. [Ding Xiaoxiao. Preparation of active ingredients of Ganoderma lucidum and its residue utilisation[D]. Zhenjiang:Jiangsu University, 2019:22-27.] Ding Xiaoxiao. Preparation of active ingredients of Ganoderma lucidum and its residue utilisation[D]. Zhenjiang: Jiangsu University, 2019: 22-27.
[13]	SHWETA D, SUMIT G, REKHA S S, et al. Debittering of bitter gourd juice using β-cyclodextrin:Mechanism and effect on antidiabetic potential[J]. Food Chemistry,2018,262(1):78−85.
[14]	王佳瑶, 李源, 李一鸣, 等. 玉米副产物发酵饮料对链脲佐菌素诱导的糖尿病小鼠糖脂代谢的改善作用[J]. 食品工业科技,2023,44(4):395−402. [WANG Jiayao, LI Yuan, LI Yiming, et al. Improvement of glycolipid metabolism in streptozotocin-induced diabetic mice by corn by-product fermented beverage[J]. Food Industry Science and Technology,2023,44(4):395−402.] WANG Jiayao, LI Yuan, LI Yiming, et al. Improvement of glycolipid metabolism in streptozotocin-induced diabetic mice by corn by-product fermented beverage[J]. Food Industry Science and Technology, 2023, 44（4）: 395−402.
[15]	常松林, 高晓余, 柳双凤, 等. α-亚麻酸对高脂饮食和链脲佐菌素诱导Ⅱ型糖尿病模型小鼠的降血糖作用[J]. 中国食品学报,2021,21(10):86−94. [CHANG Songlin, GAO Xiaoyu, LIU Shuangfeng, et al. Hypoglycaemic effects of α-linolenic acid in mice with high-fat diet and streptozotocin-induced type II diabetes[J]. Chinese Journal of Food,2021,21(10):86−94.] CHANG Songlin, GAO Xiaoyu, LIU Shuangfeng, et al. Hypoglycaemic effects of α-linolenic acid in mice with high-fat diet and streptozotocin-induced type II diabetes[J]. Chinese Journal of Food, 2021, 21（10）: 86−94.
[16]	WEI Xiaoyan, TAO Jinhua, SHEN Yumeng, et al. Sanhuang Xiexin Tang ameliorates type 2 diabetic rats via modulation of the metabolic profiles and NF-κB/PI-3K/Akt signaling pathways[J]. Frontiers in Pharmacology,2018,9:955−971. doi: 10.3389/fphar.2018.00955
[17]	GUO Xiaoxuan, WANG Yong, WANG Kai, et al. Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection[J]. Journal of Zhejiang University-Science B,2018,19(7):559−569. doi: 10.1631/jzus.B1700254
[18]	MARC A B, ABDALLAH D, KHALIL H, et al. Microvascular vasodilator properties of the angiotensin II type 2 receptor in a mouse model of type 1 diabetes[J]. Scientific Reports,2017,7(1):425−456. doi: 10.1038/s41598-017-00545-6
[19]	何静, 高婉婷, 明亮, 等. 驼乳对Ⅱ型糖尿病小鼠的糖脂代谢及胰岛素抵抗的影响[J]. 中国食品学报,2019,19(3):115−120. [HE Jing, GAO Wanting, MING Liang, et al. Effects of camel's milk on glucose-lipid metabolism and insulin resistance in type II diabetic mice[J]. Chinese Journal of Food,2019,19(3):115−120.] HE Jing, GAO Wanting, MING Liang, et al. Effects of camel's milk on glucose-lipid metabolism and insulin resistance in type II diabetic mice[J]. Chinese Journal of Food, 2019, 19（3）: 115−120.
[20]	YANG Mingjun, LÜ Jinhui, YANG Jumei, et al. Effects of Codonopsis pilosula crude polysaccharides by hypoglycemic and modulating gut microbiome in a high-fat diet and streptozotocin-induced mouse model of T2DM[J]. Journal of Functional Foods,2023,111:105893. doi: 10.1016/j.jff.2023.105893
[21]	XU Tanye, LI Guodao, WANG Xiaobo, et al. Inonotus obliquus polysaccharide ameliorates serum profiling in STZ-induced diabetic mice model[J]. Biomedcentral Chemistry,2021,15(1):64.
[22]	万凤奇. 枸杞多糖调节肝脏葡萄糖产生和抗糖尿病肾炎的作用研究[D]. 兰州:中国科学院大学(中国科学院近代物理研究所), 2023:61−73. [WAN Fengqi. Regulation of hepatic glucose production and anti-diabetic nephritis by polysaccharides of Lycium barbarum[D]. Lanzhou:University of Chinese Academy of Sciences (Institute of Modern Physics, Chinese Academy of Sciences), 2023:61−73.] WAN Fengqi. Regulation of hepatic glucose production and anti-diabetic nephritis by polysaccharides of Lycium barbarum[D]. Lanzhou: University of Chinese Academy of Sciences （Institute of Modern Physics, Chinese Academy of Sciences）, 2023: 61−73.
[23]	SHAO Weimimg, XIAO Chun, YONG Tianqiao, et al. A polysaccharide isolated from Ganoderma lucidum ameliorates hyperglycemia through modulating gut microbiota in type 2 diabetic mice[J]. International Journal of Biological Macromolecules,2021,197(1):23−38.
[24]	ARMIN A, M N H, BRIAN J B, et al. Chronic kidney disease is associated with attenuated plasma metabolome response to oral glucose tolerance testing[J]. Journal of Renal Nutrition:the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation,2022,33(2):316−325.
[25]	陈嘉骏, 王颖, 桑婷婷, 等. 灵芝多糖在糖尿病及其并发症防治中的研究进展[J]. 中草药,2022,53(3):937−947. [CHEN Jiajun, WANG Ying, SAN Tingting, et al. Research progress of Ganoderma lucidum polysaccharides in the prevention and treatment of diabetes mellitus and its complications[J]. Chinese Herbal Medicine,2022,53(3):937−947.] CHEN Jiajun, WANG Ying, SAN Tingting, et al. Research progress of Ganoderma lucidum polysaccharides in the prevention and treatment of diabetes mellitus and its complications[J]. Chinese Herbal Medicine, 2022, 53（3）: 937−947.
[26]	WANG Wenshuai, ZHANG Yaohan, WANG Zhiying, et al. Ganoderma lucidum polysaccharides improve lipid metabolism against high-fat diet-induced dyslipidemia[J]. Journal of Ethnopharmacology,2023,309(12):116321−116332.
[27]	WANG Xutao, GONG Yan, ZHOU Bin, et al. Ursolic acid ameliorates oxidative stress, inflammation and fibrosis in diabetic cardiomyopathy rats[J]. Biomedicine & Pharmacotherapy,2018,97:1461−1467.
[28]	LI Xiaopeng, WU Qian, SUI Yong, et al. Dietary supplementation of A-type procyanidins from litchi pericarp improves glucose homeostasis by modulating mTOR signaling and oxidative stress in diabetic ICR mice[J]. Journal of Functional Foods,2018,44:155−165. doi: 10.1016/j.jff.2017.12.024
[29]	毛美林, 邓子言, 李万晴, 等. 一种榆黄蘑菌丝体多糖的结构表征及其降血糖作用[J]. 食品科学,2023,44(11):115−123. [MAO Meilin, Deng Ziyan, LI Wanqing, et al. Structural characterisation of a mycelial polysaccharide from Elm mushrooms and its hypoglycemic effect[J]. Food Science,2023,44(11):115−123.] doi: 10.7506/spkx1002-6630-20220715-178 MAO Meilin, Deng Ziyan, LI Wanqing, et al. Structural characterisation of a mycelial polysaccharide from Elm mushrooms and its hypoglycemic effect[J]. Food Science, 2023, 44（11）: 115−123. doi: 10.7506/spkx1002-6630-20220715-178
[30]	DONG Xuan, ZHAO Shuxiang, YIN Xiaolu, et al. Silk sericin has significantly hypoglycaemic effect in type 2 diabetic mice via anti-oxidation and anti-inflammation[J]. International Journal of Biological Macromolecules,2020,150(1):1061−1071.
[31]	ZHOU Ning, ZHAO Ye, ZHANG Lingang , et al. Protective effects of black onion polysaccharide on liver and kidney injury in T2DM rats through the synergistic impact of hypolipidemic and antioxidant abilities[J]. International Journal of Biological Macromolecules,2022,223(Pt A):378−390.