Cloning and Expression of Marine <i>α</i>-Glucosidase and Its Preparation of High Purity Panose

YU Yiqun; XUE Luzhou; NI Hao; JIANG Lei; KANG Xinxin; PAN Saikun; WANG Shujun

doi:10.13386/j.issn1002-0306.2023110185

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 158-165. > DOI: 10.13386/j.issn1002-0306.2023110185

YU Yiqun, XUE Luzhou, NI Hao, et al. Cloning and Expression of Marine α-Glucosidase and Its Preparation of High Purity Panose[J]. Science and Technology of Food Industry, 2024, 45(19): 158−165. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110185.

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Cloning and Expression of Marine α-Glucosidase and Its Preparation of High Purity Panose

YU Yiqun^{1, 2,},
XUE Luzhou^{1, 2},
NI Hao^{1, 2},
JIANG Lei^{1, 2},
KANG Xinxin^{1, 2},
PAN Saikun^{1, 2},
WANG Shujun^{1, 2, ,}

1.
Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, Jiangsu Ocean University, Lianyungang 222005, China
2.
Key Laboratory of Marine Biotechnology of Jiangsu Province, Jiangsu Ocean University, Lianyungang 222005, China

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Received Date: November 19, 2023
Available Online: July 31, 2024

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Abstract

Abstract

Panose is iso-malto-oligosaccharide contained α-1,6 glycosidic bonds, and α-glucosidase is the key enzyme to produce panose. In this study, the α-glucosidase gene in deep-sea hydrothermal vents thermophilic archaea Thermococcus siculi HJ21 was synthesized, and cloned into the vector pET29a. Then expressed in Escherichia coli BL21 and purified by His Trap HP column. The molecular weight of α-glucosidase was determined by SDS-PAGE. The enzymatic properties and transglycosylation were also investigated. The results showed that the gene of α-glucosidase was 729 bp and encoded 242 amino acids. The molecular weight of α-glucosidase was about 27.2 kDa. Its optimal temperature and pH were 40 ℃ and 6.0 respectively. When the receptor was fructose, and the ratio of fructose and maltose was 1:9, the highest yield of panose could reached 79.1% after 10 h reaction. The results provide a basis for producing high-purity panose by α-glucosidase from archaea.
- α-glucosidase,
- cloning and expression,
- iso-malto-oligosaccharide,
- trans glycoside,
- panose

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References (34)

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