Investigation on the Recognition of Split Aflatoxin M1 Aptamer

ZHENG Yekun; DU Congcong; LI Honglin; GUO Ting; ZHANG Yuhao; MA Liang

doi:10.13386/j.issn1002-0306.2023110024

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 296-306. > DOI: 10.13386/j.issn1002-0306.2023110024

ZHENG Yekun, DU Congcong, LI Honglin, et al. Investigation on the Recognition of Split Aflatoxin M1 Aptamer[J]. Science and Technology of Food Industry, 2024, 45(19): 296−306. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110024.

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Investigation on the Recognition of Split Aflatoxin M1 Aptamer

ZHENG Yekun^1,,
DU Congcong¹,
LI Honglin¹,
GUO Ting^{1, 2, 3},
ZHANG Yuhao^{1, 2},
MA Liang^{1, 2, 3, ,}

1.
College of Food Science, Southwest University, Chongqing 400715, China
2.
Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing, Chongqing 400715, China
3.
Key Laboratory of Condiment Supervision Technology for State Market Regulation, Chongqing 400715, China

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Received Date: November 05, 2023
Available Online: August 05, 2024

Graphical Abstract

Abstract

Abstract

In this work, AFM1 was chosen as the target, and the aptamer was split to solve the low sensitivity due to unstable structure of the aptamer. The recognition ability and specificity of the split aptamer were evaluated using electrochemical methods, and the binding mechanism was explored through the circular dichroism. The results showed that the split aptamer employing a 1:1 split mode exhibited the best performance in the recognition and specificity for AFM1. The circular dichroism spectrum remained unchanged. The positive peak intensity of the circular dichroism spectrum increased significantly and the negative peak moved and the intensity increased, which demonstrated that the conformation of the aptamer changed. The sensitivity was enhanced by employing split aptamers. Superior recognition elements for the construction of sensors was obtained by the fragmentation of intact aptamers. The investigation provides a new idea to enhance the sensitivity of the detection mehtods based on the apatamer.
- flatoxin M1,
- split aptamer,
- recognition ability,
- electrochemistry

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References (50)

References

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