YIN Xinya, LI Mengyao, LI Hehe, et al. Effects of Protein-glutaminase and Starter Cultures on the Quality of Walnut-based Yogurt[J]. Science and Technology of Food Industry, 2024, 45(18): 128−137. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100214.
Citation: YIN Xinya, LI Mengyao, LI Hehe, et al. Effects of Protein-glutaminase and Starter Cultures on the Quality of Walnut-based Yogurt[J]. Science and Technology of Food Industry, 2024, 45(18): 128−137. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100214.

Effects of Protein-glutaminase and Starter Cultures on the Quality of Walnut-based Yogurt

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  • Received Date: October 26, 2023
  • Available Online: July 17, 2024
  • Walnut kernels, selected as the raw material, underwent hydrolysis with protein-glutaminase (PG) following pulping. This process laid the foundation for producing set-type walnut yogurt, involving steps such as blending, homogenization, sterilization, and fermentation. The study investigated the impact of protein-glutaminase and five distinct starter cultures labeled I~V on the quality of walnut yogurt. This was evaluated by examining the yogurt's fermentation characteristics, physicochemical indicators, and microstructure. Furthermore, walnut yogurt developed under these optimal conditions was compared to traditional milk yogurt to assess its quality and characteristics. The findings revealed a significant improvement in the fermentation characteristics and textural parameters of yogurt fermented by walnut milk following enzymatic hydrolysis compared to non-enzymatic walnut milk. The optimal conditions were identified as 0.2% enzyme concentration and a 40-minute reaction time, with the pH, acidity, hardness, and consistency of walnut yogurt being 4.3, 55°T, 85.30 g, and 583.96 g·s, respectively. Walnut yogurt developed using starter culture I exhibited the highest acidity level, maintaining minimal fluctuation over a 21-day storage period. In contrast, yogurts prepared with alternative starter cultures demonstrated varying degrees of stratification and sedimentation. The yogurt fermented with culture I showed superior viscoelastic properties, presenting a denser gel network. Conversely, yogurts fermented with cultures IV and V displayed reduced apparent viscosity, lower values of G' (storage modulus), and G'' (loss modulus), along with a looser gel structure. Additionally, walnut yogurt produced with starter culture I maintained bacterial counts exceeding 106 CFU/mL throughout the storage duration. Despite walnut yogurt requiring further refinement in aspects such as consistency, water-holding capacity, and whey release rate compared to milk yogurt, the overall impact of PG was positive, enhancing the gelation quality of walnut yogurt. The yogurt prepared with starter culture I (Lactobacillus bulgaricus and Streptococcus thermophilus) was notably improved, receiving favorable overall assessments.
  • [1]
    KONG X Z, ZHANG L N, LU X, et al. Effect of high-speed shearing treatment on dehulled walnut proteins[J]. LWT-Food Science and Technology,2019,116:108500. doi: 10.1016/j.lwt.2019.108500
    [2]
    ZHANG G Q, MA S J, LIU X, et al. Protein-glutaminase:Research progress and prospect in food manufacturing[J]. Food Bioscience,2021,43:101314. doi: 10.1016/j.fbio.2021.101314
    [3]
    CHEN X, FU W Y, LUO Y C, et al. Protein deamidation to produce processable ingredients and engineered colloids for emerging food applications[J]. Comprehensive Reviews in Food Science and Food Safety,2021,20(4):3788−3817. doi: 10.1111/1541-4337.12759
    [4]
    MA S J, LIU X, ZHOU J W, et al. Characterization of high internal phase emulsions stabilized by protein glutaminase-deamidated wheat gluten[J]. LWT-Food Science and Technology,2023,179:114622. doi: 10.1016/j.lwt.2023.114622
    [5]
    ZHONG Q J, SONTAG S T, SALOVAARA H, et al. Oat protein solubility and emulsion properties improved by enzymatic deamidation[J]. Journal of Cereal Science,2015,64:126−132. doi: 10.1016/j.jcs.2015.04.010
    [6]
    JIANG Y Q, WANG Z J, HE Z Y, et al. Effect of heat-induced aggregation of soy protein isolate on protein-glutaminase deamidation and the emulsifying properties of deamidated products[J]. 2022, 154:112328.
    [7]
    FANG L Y, XIANG H, SUN-WATERHOUSE D X, et al. Enhancing the usability of pea protein isolate in food applications through modifying its structural and sensory properties via deamidation by glutaminase[J]. Agricultural and Food Chemistry,2020,68:1691−1697. doi: 10.1021/acs.jafc.9b06046
    [8]
    MIWA N, YOKOYAMA K, WAKABAYASHI H, et al. Effect of deamidation by protein-glutaminase on physicochemical and functional properties of skim milk[J]. International Dairy Journal,2010,20(6):393−399. doi: 10.1016/j.idairyj.2009.12.015
    [9]
    WU J J, DAI T, LIN R Y, et al. Effect of protein-glutaminase on the texture, rheology, microstructure and sensory properties of skimmed set-type yoghurt[J]. Food Chemistry,2023,429:136831. doi: 10.1016/j.foodchem.2023.136831
    [10]
    马文艺, 李兴飞, 刘军, 等. 制浆工艺和发酵剂分别对豌豆浆风味和豌豆酸奶品质的影响[J]. 食品与发酵工业,2021,47(20):188−193. [MA W Y, LI X F, LIU J, et al. The effects of pulping process and starter on the flavor of pea milk and the quality of pea yogurt respectively[J]. Food and Fermentation Industries,2021,47(20):188−193.]

    MA W Y, LI X F, LIU J, et al. The effects of pulping process and starter on the flavor of pea milk and the quality of pea yogurt respectively[J]. Food and Fermentation Industries, 2021, 47(20): 188−193.
    [11]
    林浩鹏, 刘亚琼, 梁梦帆, 等. 商品发酵剂对凝固型核桃酸奶品质的影响研究[J]. 中国乳品工业,2020,48(4):61−64. [LIN H P, LIU Y Q, LIANG M F, et al. Study on the effect of commercial starter on the quality of solidified walnut yoghurt[J]. China Dairy Industry,2020,48(4):61−64.]

    LIN H P, LIU Y Q, LIANG M F, et al. Study on the effect of commercial starter on the quality of solidified walnut yoghurt[J]. China Dairy Industry, 2020, 48(4): 61−64.
    [12]
    LEVY R, OKUN Z, DAVIDOVICH-PINHAS M, et al. Utilization of high-pressure homogenization of potato protein isolate for the production of dairy-free yogurt-like fermented product[J]. Food Hydrocolloids,2021,113:106442. doi: 10.1016/j.foodhyd.2020.106442
    [13]
    MIWA N, NIO N, SONOMOTO K. Effect of enzymatic deamidation by protein-glutaminase on the textural and microstructural properties of set yoghurt[J]. International Dairy Journal,2014,36(1):1−5. doi: 10.1016/j.idairyj.2013.12.002
    [14]
    HUANG K, LIU Y Y, ZHANG Y, et al. Formulation of plant-based yoghurt from soybean and quinoa and evaluation of physicochemical, rheological, sensory and functional properties[J]. Food Bioscience,2022,49:101831. doi: 10.1016/j.fbio.2022.101831
    [15]
    CHEN B F, ZHAO X J, CAI Y J, et al. Incorporation of modified okara-derived insoluble soybean fiber into set-type yogurt:structural architecture, rheological properties and moisture stability[J]. Food Hydrocolloids,2023,137:108413. doi: 10.1016/j.foodhyd.2022.108413
    [16]
    斯梦, 孔祥珍, 张彩猛, 等. 不同糖类和蛋白质浓度对核桃乳发酵及其感官特性的影响[J]. 食品与发酵工业,2022,48(21):111−117. [SI M, KONG X Z, ZHANG C M, et al. Effects of sugar varieties and protein concentration on fermentation and sensory characteristics of fermented walnut milk[J]. Food and Fermentation Industries,2022,48(21):111−117.]

    SI M, KONG X Z, ZHANG C M, et al. Effects of sugar varieties and protein concentration on fermentation and sensory characteristics of fermented walnut milk[J]. Food and Fermentation Industries, 2022, 48(21): 111−117.
    [17]
    孙乾. 改性对核桃谷蛋白结构表征及功能特性的影响[D]. 乌鲁木齐:新疆农业大学, 2019. [SUN Q. Effects of modification on structural characterization and functional properties of walnut glutenin[D]. Urumqi:Xinjiang Agricultural University.]

    SUN Q. Effects of modification on structural characterization and functional properties of walnut glutenin[D]. Urumqi: Xinjiang Agricultural University.
    [18]
    ZHAO J, BHANDARI B, GAIANI C, et al. Physicochemical and microstructural properties of fermentation-induced almond emulsion-filled gels with varying concentrations of protein, fat and sugar contents[J]. Current Research in Food Science,2021,4:577−587. doi: 10.1016/j.crfs.2021.08.007
    [19]
    李锋, 华欲飞. 应用不同类型的大豆蛋白制备大豆酸奶[J]. 无锡轻工大学学报,2004,23(3):61−64. [LI F, HUA Y F. Different types of soy protein were used to prepare soy yogurt[J]. Journal of Wuxi University of Light Industry,2004,23(3):61−64.]

    LI F, HUA Y F. Different types of soy protein were used to prepare soy yogurt[J]. Journal of Wuxi University of Light Industry, 2004, 23(3): 61−64.
    [20]
    韩芸娇, 梁玲, 邱雯雯, 等. 费氏丙酸杆菌与乳酸菌混合发酵酸奶研究[J]. 中国食品学报,2017,17(3):96−103. [HAN Y J, LIANG L, QIU W W, et al. Studies on fermented yogurt using Propionibacterium freudenreichii and Lactobacillus[J]. Journal of Chinese Institute of Food Science and Technology,2017,17(3):96−103.]

    HAN Y J, LIANG L, QIU W W, et al. Studies on fermented yogurt using Propionibacterium freudenreichii and Lactobacillus[J]. Journal of Chinese Institute of Food Science and Technology, 2017, 17(3): 96−103.
    [21]
    SHORI A B, ALJOHANI G S, AL-ZAHRANI A J, et al. Viability of probiotics and antioxidant activity of cashew milk-based yogurt fermented with selected strains of probiotic Lactobacillus spp.[J]. LWT-Food Science and Technology,2022,153:112482. doi: 10.1016/j.lwt.2021.112482
    [22]
    GHARAIE Z, AZIZI M H, BARZEGAR M, et al. Gum tragacanth oil/gels as an alternative to shortening in cookies:Rheological, chemical and textural properties[J]. LWT-Food Science and Technology,2019,105:265−271. doi: 10.1016/j.lwt.2019.02.025
    [23]
    CUI H T, LI J, XU X Y, et al. Enzymatic interesterification of beef tallow/coconut oil blends to produce a superior margarine base stock[J]. International Journal of Food Science and Technology,2021,57(2):908−919.
    [24]
    MASALOVA I, KAPIAMBA F, TSHILUMBU N, et al. Shear stability of highly concentrated emulsions[J]. Colloid Journal,2018,80(1):54−58. doi: 10.1134/S1061933X18010064
    [25]
    KLOST M, RIBES G G, DRUSCH S. Enzymatic hydrolysis of pea protein:Interactions and protein fractions involved in fermentation induced gels and their influence on rheological properties[J]. Food Hydrocolloids,2020,105:105793. doi: 10.1016/j.foodhyd.2020.105793
    [26]
    XU X Y, CUI H T, YUAN Z H, et al. Effects of different combinations of probiotics on rheology, microstructure, and moisture distribution of soy materials-based yogurt[J]. Journal of Food Science,2022,87(7):2820−2830. doi: 10.1111/1750-3841.16204
    [27]
    吴俊霞, 程建明, 薛峰. 枸杞大豆蛋白植物基酸奶理化性质研究[J]. 食品与发酵工业,2023,49(10):193−198. [WU J X, CHENG J M, XUE F. Physiochemical properties of plant-based yogurt prepared by wolfberry and soy protein[J]. Food and Fermentation Industries,2023,49(10):193−198.]

    WU J X, CHENG J M, XUE F. Physiochemical properties of plant-based yogurt prepared by wolfberry and soy protein[J]. Food and Fermentation Industries, 2023, 49(10): 193−198.
    [28]
    韩永佳. 功能型酸奶的理化特性、抗氧化活性及微观结构的研究[D]. 合肥:合肥工业大学, 2016. [HAN Y J. Functional of yogurt on the physicochemical properties, antioxidant activity, and microstructure were studied[D]. Hefei:Hefei University of Technology, 2016.]

    HAN Y J. Functional of yogurt on the physicochemical properties, antioxidant activity, and microstructure were studied[D]. Hefei: Hefei University of Technology, 2016.
    [29]
    MIELE N A, CABISIDAN E K, BLAIOTTA G, et al. Rheological and sensory performance of a protein-based sweetener (MNEI), sucrose, and aspartame in yogurt[J]. International Dairy Journal,2017,100(12):9539−9550.
    [30]
    GILBERT A, RIOUX L E, ST-GELAIS D, et al. Characterization of syneresis phenomena in stirred acid milk gel using low frequency nuclear magnetic resonance on hydrogen and image analyses[J]. Food Hydrocolloids,2020,106:105907. doi: 10.1016/j.foodhyd.2020.105907
    [31]
    JØRGENSEN C E, ABRAHAMSEN R K, RUKKE E O, et al. Processing of high-protein yoghurt-a review[J]. International Dairy Journal,2019,88:42−59. doi: 10.1016/j.idairyj.2018.08.002
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