Protective Effects of Enzymatic Products from Sturgeon Roe on Alcohol-induced Hepatic Cell Damage and Virtual Screening of Active Peptides

ZHANG Lijun; ZHAO Tiantian; CHEN Jieqiong; ZHONG Kangrong; GUAN Yongjian; LUO Rui; LIN Chunyan; NAN Haijun; ZHANG Yehui

doi:10.13386/j.issn1002-0306.2023100023

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 316-324. > DOI: 10.13386/j.issn1002-0306.2023100023

ZHANG Lijun, ZHAO Tiantian, CHEN Jieqiong, et al. Protective Effects of Enzymatic Products from Sturgeon Roe on Alcohol-induced Hepatic Cell Damage and Virtual Screening of Active Peptides[J]. Science and Technology of Food Industry, 2024, 45(19): 316−324. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100023.

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Protective Effects of Enzymatic Products from Sturgeon Roe on Alcohol-induced Hepatic Cell Damage and Virtual Screening of Active Peptides

ZHANG Lijun^{1, 2,},
ZHAO Tiantian²,
CHEN Jieqiong²,
ZHONG Kangrong^{3, 4},
GUAN Yongjian^{3, 4},
LUO Rui^{3, 4},
LIN Chunyan^{1, 2},
NAN Haijun^1, ,,
ZHANG Yehui^2, ,

1.
School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China
2.
Sericultural & Agri-food Research Institute, Guangdong Academy of Agriculture Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510640, China
3.
Guizhou Qianxun Biological Technology Co., Ltd., Qiandongnan 556399, China
4.
Guangdong Bixiang Technological Development Co., Ltd., Qingyuan 513023, China

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Received Date: October 08, 2023
Available Online: July 31, 2024

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Abstract

Abstract

This study was designed to explore the hepatoprotective effects of enzymatic degradation products derived from sturgeon roe on alcohol-damaged hepatocytes and to elucidate the underlying mechanisms. Utilizing sturgeon roe as the research material, the enzymatic process was optimized by focusing on key parameters such as enzymatic efficiency and in vitro activity. The protective effects on hepatocytes were systematically investigated by using the HepG2 cell model. Advanced computerized virtual screening technology was employed to identify potential bioactive peptides within the sturgeon roe. The findings indicated that the optimum enzymatic product of sturgeon roe was obtained under the conditions of 1:1 compounding of alkaline protease (1343 U/g) and trypsin (27 U/g) for 8 h, yielded a protein recovery of 49.30%±0.57%, a hydrolysis degree of 46.05%±0.92%, and ABTS⁺ free radical scavenging rate of 51.45%±0.66%. Notably, at a concentration of 5 mg/mL, this product significantly enhanced the activity of alcohol dehydrogenase (ADH) by 168.64%±1.42% (P<0.05). The product prepared under this enzymatic condition at 0.3~2 mg/mL significantly elevated superoxide dismutase (SOD) activity, glutathione (GSH) contents, and reduced malondialdehyde (MDA) contents in alcohol-injured HepG2 cells compared to the model group (P<0.05). In addition, the product at 0.3~2 mg/mL also significantly reduced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities (P<0.05). Virtual screening identified 5 peptides, including LPG and FLPR, in the sturgeon roe enzymatic degradation product, showed promising hepatoprotective properties against alcohol-induced hepatocyte injury. Consequently, this study lays a solid foundation for the further development and utilization of aquatic resources, such as sturgeon roe, in hepatoprotective applications.
- sturgeon,
- sturgeon roe,
- alcohol dehydrogenase,
- antioxidation,
- bioactive peptides,
- alcohol metabolism

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References (31)

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