Optimization of Extraction Process of Polysaccharides from <i>Microcos paniculata</i> and Its Biological Activity Analysis

LIU Qiuye; LIU Hui; CHEN Xin; ZUO Yajie

doi:10.13386/j.issn1002-0306.2023090110

Volume 45 Issue 4

Feb. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(4): 197-204. > DOI: 10.13386/j.issn1002-0306.2023090110

LIU Qiuye, LIU Hui, CHEN Xin, et al. Optimization of Extraction Process of Polysaccharides from Microcos paniculata and Its Biological Activity Analysis[J]. Science and Technology of Food Industry, 2024, 45(4): 197−204. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090110.

Citation:

PDF (1617 KB)

Optimization of Extraction Process of Polysaccharides from Microcos paniculata and Its Biological Activity Analysis

1.
The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China
2.
School of Medicine, Foshan University, Foshan 528225, China

More Information

Received Date: September 12, 2023
Available Online: December 11, 2023

Graphical Abstract

Abstract

Abstract

Objective: To optimize the extraction process of polysaccharides from Microcos paniculata, and evaluate the antioxidant, antibacterial and pancreatic lipase inhibitory activities of Microcos polysaccharide. Methods: Ultrasonic-assisted extraction method was employed to extract polysaccharides from Microcos paniculata. The single-factor experiments were combined with orthogonal experiment to optimize the parameters of the extraction process. Antioxidant activity of Microcos polysaccharide was evaluated by DPPH, ABTS⁺, and OH free radical scavenging assays. The pancreatic lipase test was used to study the lipid lowering activity of Microcos polysaccharide. In addition, the antibacterial activity of Microcos polysaccharide against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae were measured by the micro broth dilution method. Results: The optimum conditions of Microcos polysaccharide were as follows: Ultrasonic power was 225 W, extraction temperature was 65 ℃, solid-liquid ratio was 1:20 (g/mL), extraction time was 35 min. Under these conditions, the yield of Microcos polysaccharide was 3.76%±0.25%. Microcos polysaccharide exhibited potent scavenging activity on DPPH, ABTS⁺ and OH free radicals, with the half-inhibitory concentrations were 0.1430, 0.01553 and 0.1451 mg/mL, respectively. The inhibition rate of pancreatic lipase was 36.22%±1.76%, when the concentration of Microcos polysaccharide was 5 mg/mL. The minimum inhibitory concentrations of Microcos polysaccharide against P. aeruginosa was 50 mg/mL, against E. coli and K. pneumoniae were all 100 mg/mL, against A. baumannii was 200 mg/mL. Conclusion: The extraction process optimized by single factor experiments combined with orthogonal experiment could effectively extract Microcos polysaccharides. The Microcos polysaccharide revealed good antioxidant and antibacterial as well as potential pancreatic lipase inhibitory activities, which would be valuable for in-depth developmental research.
- Microcos polysaccharide,
- extraction process,
- antioxidant activity,
- antibacterial activity,
- pancreatic lipase inhibitory activity

FullText(HTML)

References (41)

References

[1]	国家药典委员会. 中国药典[M]. 一部. 北京:中国医药科技出版社, 2020:95. [National Pharmacopoeia Commission, Chinese Pharmacopoeia[M]. Part1. Beijing:China Medical Science and Technology Press, 2020:95.] National Pharmacopoeia Commission, Chinese Pharmacopoeia[M]. Part1. Beijing: China Medical Science and Technology Press, 2020: 95.
[2]	严翠, 庞晓妍, 范卫锋, 等. 基于网络药理学和分子对接探究布渣叶改善消化不良的作用机制[J]. 现代药物与临床, 2023, 38(6):1335−1343. [YAN C, PANG X Y, FAN W F, et al. Mechanism of leaves of Microcos paniculata improving of dyspepsia based on network pharmacology and molecular docking[J]. Drugs & Clinic, 2023, 38(6):1335−1343.] YAN C, PANG X Y, FAN W F, et al. Mechanism of leaves of Microcos paniculata improving of dyspepsia based on network pharmacology and molecular docking[J]. Drugs & Clinic, 2023, 38（6）: 1335−1343.
[3]	杨琳. 仙草爽凉茶的研制[J]. 饮料工业, 1999(6):29-32. [YANG L. The production of cool tea from immortal grass[J]. Beverage Industry, 1999(6):29-32.] YANG L. The production of cool tea from immortal grass[J]. Beverage Industry, 1999（6）: 29-32.
[4]	WANG J, ZHOU B, HU X, et al. Deciphering the formulation secret underlying Chinese Huo-clearing herbal drink[J]. Frontiers in Pharmacology,2021,12:654699. doi: 10.3389/fphar.2021.654699
[5]	BAI Y, JIA Q, SU W, et al. Integration of molecular networking and fingerprint analysis for studying constituents in Microctis Folium[J]. PLoS One,2020,15(7):e235533.
[6]	ZHANG G, ZHANG N, XU L, et al. A new piperidine alkaloid from the leaves of Microcos paniculata L[J]. Natural Product Research,2017,31(2):169−174. doi: 10.1080/14786419.2016.1224868
[7]	孙冬梅, 汪梦霞. 布渣叶化学成分和药理作用研究进展[J]. 世界中医药, 2015, 10(1):143-147. [SUN D M, WANG M X, Advanceson chemical constituents and pharmacological effects of Microcos paniculata[J]. World Chinese Medicine, 2015, 10(1):143-147.] SUN D M, WANG M X, Advanceson chemical constituents and pharmacological effects of Microcos paniculata[J]. World Chinese Medicine, 2015, 10（1）: 143-147.
[8]	肖观林, 江洁怡, 李素梅, 等. 基于UPLC-Q-TOF-MS/MS技术的布渣叶化学成分分析[J]. 中国实验方剂学杂志,2021,27(3):138−148. [XIAO G L, JIANG J Y, LI S M, et al. Analysis of chemical constituents in Microctis Folium by UPLC-Q-TOF-MS/MS[J]. Chinese Journal of Experimental Traditional Medical Formulae,2021,27(3):138−148.] XIAO G L, JIANG J Y, LI S M, et al. Analysis of chemical constituents in Microctis Folium by UPLC-Q-TOF-MS/MS[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2021, 27（3）: 138−148.
[9]	李振雨, 何民友, 刘晓霞, 等. 基于UPLC指纹图谱和多成分定量的布渣叶饮片和标准汤剂量值传递研究[J]. 天然产物研究与开发,2023,35(9):1505−1517. [LI Z Y, HE M Y, LIU X X, et al. Study on the quantity value transfer of microctis folium decoction pieces and standard decoction based on UPLC fingerprint and multi-component quantification[J]. Natural Product Research and Development,2023,35(9):1505−1517.] LI Z Y, HE M Y, LIU X X, et al. Study on the quantity value transfer of microctis folium decoction pieces and standard decoction based on UPLC fingerprint and multi-component quantification[J]. Natural Product Research and Development, 2023, 35（9）: 1505−1517.
[10]	曾聪彦, 钟希文, 高玉桥, 等. 布渣叶水提物对小鼠及大鼠胃肠功能的影响[J]. 今日药学,2009,19(8):11−12. [ZENG C Y, ZHONG X W, GAO Y Q, et al. Effects of water extracted on Microcos paniculata on gastrointestinal function in mice and rats[J]. Pharmacy Today,2009,19(8):11−12.] ZENG C Y, ZHONG X W, GAO Y Q, et al. Effects of water extracted on Microcos paniculata on gastrointestinal function in mice and rats[J]. Pharmacy Today, 2009, 19（8）: 11−12.
[11]	曾聪彦, 梅全喜, 高玉桥, 等. 布渣叶水提物解热退黄作用的实验研究[J]. 中国药房, 2010, 21(11):973-974. [ZENG C Y, MEI Q X, GAO Y Q, et al. Experimental study of antipyretic and jaundice-relieving effect of water extract of Microcos paniculata[J] China Pharmacy, 2010, 21(11):973-974.] ZENG C Y, MEI Q X, GAO Y Q, et al. Experimental study of antipyretic and jaundice-relieving effect of water extract of Microcos paniculata[J] China Pharmacy, 2010, 21（11）: 973-974.
[12]	曾聪彦, 梅全喜, 高玉桥, 等. 布渣叶水提物镇痛药效学的实验研究[J]. 中华中医药学刊, 2009, 27(8):1757-1758. [ZENG C Y, MEI Q X, GAO Y Q, et al. Experinental study on the pharmacodynamcis in analgesic of water-extract of Mcirocos paniculata[J] Chinese Archives of Traditional Chinese Medicine, 2009, 27(8):1757-1758.] ZENG C Y, MEI Q X, GAO Y Q, et al. Experinental study on the pharmacodynamcis in analgesic of water-extract of Mcirocos paniculata[J] Chinese Archives of Traditional Chinese Medicine, 2009, 27（8）: 1757-1758.
[13]	CHENG Y, XUE F, YANG Y. Hot water extraction of antioxidants from tea leaves-optimization of brewing conditions for preparing antioxidant-rich tea drinks[J]. Molecules,2023,28(7):3030. doi: 10.3390/molecules28073030
[14]	BAGADE S, PATIL M. Recent advances in microwave assisted extraction of bioactive compounds from complex herbal samples:A Review[J]. Critical Reviews in Analytical Chemistry,2021,51(2):138−149. doi: 10.1080/10408347.2019.1686966
[15]	REN Z, LIU W, SONG X, et al. Antioxidant and anti-inflammation of enzymatic-hydrolysis residue polysaccharides by Lentinula edodes[J]. International Journal of Biological Macromolecules, 2018 , 120(Pt A):811-822.
[16]	WANG Y, XIONG X, HUANG G. Ultrasound-assisted extraction and analysis of maidenhairtree polysaccharides[J]. Ultrasonics Sonochemistry,2023,95:106395. doi: 10.1016/j.ultsonch.2023.106395
[17]	CHEN Y, ZHANG J, LI Q, et al. Response surface methodology for optimizing the ultrasound-assisted extraction of polysaccharides from Acanthopanax giraldii[J]. Chemical & Pharmaceutical Bulletin,2018,66(8):785−793.
[18]	张双奇, 刘琳, 何念武, 等. 超声辅助提取陕产天麻多糖的工艺优化及抗氧化活性研究[J]. 中国农学通报,2021,37(9):131−136. [ZHANG S Q, LIU L, HE N W, et al. Gastrodia elata polysaccharide from Shanxi by ultrasound-assisted hot water extraction process optimization and antioxidant activity[J]. Chinese Agricultural Science Bulletin,2021,37(9):131−136.] ZHANG S Q, LIU L, HE N W, et al. Gastrodia elata polysaccharide from Shanxi by ultrasound-assisted hot water extraction process optimization and antioxidant activity[J]. Chinese Agricultural Science Bulletin, 2021, 37（9）: 131−136.
[19]	黄先敏, 凌敏, 杨正贤, 等. 不同产地天麻多糖含量测定分析[J]. 现代农业科技,2017(6):259−263. [HUANG X M, LING M, YANG Z X, et al. Determination and analysis of polysaccharide content of Gastrodia elata from different producing areas[J]. Modern Agricultural Science and Technology,2017(6):259−263.] HUANG X M, LING M, YANG Z X, et al. Determination and analysis of polysaccharide content of Gastrodia elata from different producing areas[J]. Modern Agricultural Science and Technology, 2017（6）: 259−263.
[20]	金蓉, 谢文华, 陈杰标, 等. 超声辅助热水提取对铁皮石斛水溶性多糖得率和结构的影响[J]. 浙江大学学报(农业与生命科学版),2019,45(2):196−204. [JIN R, XIE W H, CHEN J B, et al. Effects of ultrasonic-assisted hot water extraction on the yield and structure of water soluble polysaccharides from Dendrobium officinale[J]. Journal of Zhejiang University (Agriculture & Life Sciences),2019,45(2):196−204.] doi: 10.3785/j.issn.1008-9209.2018.04.161 JIN R, XIE W H, CHEN J B, et al. Effects of ultrasonic-assisted hot water extraction on the yield and structure of water soluble polysaccharides from Dendrobium officinale[J]. Journal of Zhejiang University （Agriculture & Life Sciences）, 2019, 45（2）: 196−204. doi: 10.3785/j.issn.1008-9209.2018.04.161
[21]	CHEN X, ZHANG H, DU W, et al. Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge[J]. International Journal of Biological Macromolecules,2020,150:1011−1019. doi: 10.1016/j.ijbiomac.2019.11.056
[22]	孙宁云, 姚欣, 张英慧, 等. 鸡蛋花多糖提取工艺优化及生物活性研究[J]. 食品工业科技,2022,43(3):180−187. [SUN N Y, YAO X, ZHANG Y H, et al. [Optimization of extraction process of polysaccharides from Plumeria rubra L. cv. Acutifolia and evaluation of biological activity[J]. Science and Technology of Food Industry,2022,43(3):180−187.] SUN N Y, YAO X, ZHANG Y H, et al. [Optimization of extraction process of polysaccharides from Plumeria rubra L. cv. Acutifolia and evaluation of biological activity[J]. Science and Technology of Food Industry, 2022, 43（3）: 180−187.
[23]	钱燕芳, 石晨莹, 陈贵堂. 桑葚多糖超声提取、脱色工艺优化及其抗氧化活性分析[J]. 食品工业科技,2022,43(16):201−210. [QIAN Y F, SHI C Y, CHEN G T. Optimization of ultrasound-assisted extraction and decolorization process of polysaccharides from Mori fructus and its antioxidant activity[J]. Science and Technology of Food Industry,2022,43(16):201−210.] QIAN Y F, SHI C Y, CHEN G T. Optimization of ultrasound-assisted extraction and decolorization process of polysaccharides from Mori fructus and its antioxidant activity[J]. Science and Technology of Food Industry, 2022, 43（16）: 201−210.
[24]	姚欣, 孙宁云, 陈鑫, 等. 山竹壳多糖提取工艺优化及其生物活性研究[J]. 食品研究与开发,2022,43(15):101−108. [YAO X, SUN N Y, CHEN X, et al. Optimization of the extraction of mangosteen ( Garcinia mangostana) pericarp polysaccharides and determination of their biological activities[J]. Food Research and Development,2022,43(15):101−108.] YAO X, SUN N Y, CHEN X, et al. Optimization of the extraction of mangosteen （Garcinia mangostana） pericarp polysaccharides and determination of their biological activities[J]. Food Research and Development, 2022, 43（15）: 101−108.
[25]	宋英, 周小初, 宋崎, 等. 党参中多糖的含量测定方法研究[J]. 中国药业,2008,17(23):9−10. [[SONG Y, ZHOU X C, SONG Q, et al. Study on content determination of polysaccharidein from Radix Codonopsis[J]. China Pharmaceuticals,2008,17(23):9−10.] doi: 10.3969/j.issn.1006-4931.2008.23.005 [SONG Y, ZHOU X C, SONG Q, et al. Study on content determination of polysaccharidein from Radix Codonopsis[J]. China Pharmaceuticals, 2008, 17（23）: 9−10. doi: 10.3969/j.issn.1006-4931.2008.23.005
[26]	SHEN S G, JIA S R, WU Y K, et al. Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme[J]. Carbohydrate Polymers,2018,198:426−433. doi: 10.1016/j.carbpol.2018.06.111
[27]	TANG Q, HUANG G. Preparation and antioxidant activities of cuaurbit polysaccharide[J]. International Journal of Biological Macromolecules,2018,117:362−365. doi: 10.1016/j.ijbiomac.2018.05.213
[28]	王迦琦, 许梦然, 高婧文, 等. 北虫草多糖提取工艺优化及其细胞氧化损伤保护作用[J]. 食品工业科技,2020,41(13):141−147. [WANG J Q, XU M R, GAO J W, et al. Optimization of extraction process of Cordyceps militaris polysaccharide and its protective effect on cellular oxidative damage[J]. Science and Technology of Food Industry,2020,41(13):141−147.] WANG J Q, XU M R, GAO J W, et al. Optimization of extraction process of Cordyceps militaris polysaccharide and its protective effect on cellular oxidative damage[J]. Science and Technology of Food Industry, 2020, 41（13）: 141−147.
[29]	裴文清, 吕泸楠, 王靖宇, 等. 木瓜皮多酚和黄酮提取工艺优化及酪氨酸酶与胰脂肪酶抑制活性研究[J]. 食品工业科技,2022,43(1):188−195. [PEI W Q, LÜ L N, WANG J Y, et al. Optimization of extraction of polyphenols and flavonoids from Papaya peel and its anti-tyrosinase and anti-pancreatic lipase[J]. Science and Technology of Food Industry,2022,43(1):188−195.] PEI W Q, LÜ L N, WANG J Y, et al. Optimization of extraction of polyphenols and flavonoids from Papaya peel and its anti-tyrosinase and anti-pancreatic lipase[J]. Science and Technology of Food Industry, 2022, 43（1）: 188−195.
[30]	曲词, 任丹丹, 任先见, 等. 海黍子多酚提取工艺的优化及抑菌活性研究[J]. 食品工业,2016,37(11):169−172. [QU C, REN D D, REN X J, et al. Study on extraction process of polyphenols from Sargassum kjellmanianum and anti-microbial activities[J]. The Food Industry,2016,37(11):169−172.] QU C, REN D D, REN X J, et al. Study on extraction process of polyphenols from Sargassum kjellmanianum and anti-microbial activities[J]. The Food Industry, 2016, 37（11）: 169−172.
[31]	常庆, 吴少雄, 王琦, 等. 超声波辅助提取、纯化滇橄榄多糖的工艺研究[J]. 食品安全质量检测学报,2022,13(6):1755−1763. [CHANG Q, WU S X, WANG Q, et al. Study on process of ultrasonic assisted extraction and purification of polysaccharides from Phyllanthus emblica Linn J]. Journal of Food Safety and Quality,2022,13(6):1755−1763.]
[32]	李晨伟, 李国强, 范丽霞, 等. 木棉花多糖提取工艺优化[J]. 湖南农业科学,2023(3):77−80. [LI C W, LI G Q, FAN L X, et al. Extraction process optimization of Bombax ceiba polysaccharides[J]. Hunan Agricultural Sciences,2023(3):77−80.] doi: 10.16498/j.cnki.hnnykx.2023.003.016 LI C W, LI G Q, FAN L X, et al. Extraction process optimization of Bombax ceiba polysaccharides[J]. Hunan Agricultural Sciences, 2023（3）: 77−80. doi: 10.16498/j.cnki.hnnykx.2023.003.016
[33]	徐瑾钰, 彭小伟, 阚欢, 等. 滇皂角米多糖提取工艺优化及其抗氧化活性[J]. 食品研究与开发,2023,44(14):112−118. [XU J Y, PENG X W, KAN H, et al. Optimization of extraction process and antioxidant activity of polysaccharide from the seed of Gleditsia japonica[J]. Food Research and Development,2023,44(14):112−118.] doi: 10.12161/j.issn.1005-6521.2023.14.017 XU J Y, PENG X W, KAN H, et al. Optimization of extraction process and antioxidant activity of polysaccharide from the seed of Gleditsia japonica[J]. Food Research and Development, 2023, 44（14）: 112−118. doi: 10.12161/j.issn.1005-6521.2023.14.017
[34]	苗晶囡, 董嘉木, 刘树民, 等. 黑皮鸡枞菌多糖超声辅助提取条件优化及抗氧化活性研究[J]. 食品安全质量检测学报,2023,14(12):109−116. [MIAO J N, DONG J M, LIU S M, et al. Ultrasonic-assisted extraction condition optimization and antioxidant activity of polysaccharides from Oudemansiella raphanipes[J]. Journal of Food Safety and Quality,2023,14(12):109−116.] MIAO J N, DONG J M, LIU S M, et al. Ultrasonic-assisted extraction condition optimization and antioxidant activity of polysaccharides from Oudemansiella raphanipes[J]. Journal of Food Safety and Quality, 2023, 14（12）: 109−116.
[35]	王敏, 于姝燕, 钮松召, 等. 蒙药漏芦花多糖提取工艺及其抗氧化抗菌活性研究[J]. 化学研究与应用, 2019, 31(3):464−469. [WANG M, YU S Y, NIU S Z, et al. Study on optimization of extraction technology, antioxidant and antibacterial activity of polysaccharide from Mongolian medicine Rhaponticum uniflorum [J]. Chemical Research and Application, 2019, 31(3):464−469.] WANG M, YU S Y, NIU S Z, et al. Study on optimization of extraction technology, antioxidant and antibacterial activity of polysaccharide from Mongolian medicine Rhaponticum uniflorum [J]. Chemical Research and Application, 2019, 31（3）: 464−469.
[36]	TU J Q, LIU H P, WEN Y H, et al. A novel polysaccharide from Hericium erinaceus:Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro[J]. Journal of Food Biochemistry,2021,45(9):e13871.
[37]	GU J, ZHANG H, WEN C, et al. Purification, characterization, antioxidant and immunological activity of polysaccharide from Sagittaria sagittifolia L[J]. Food Research International,2020,136:109345. doi: 10.1016/j.foodres.2020.109345
[38]	ZHOU H, DAI C, CUI X, et al. Immunomodulatory and antioxidant effects of Glycyrrhiza uralensis polysaccharide in Lohmann Brown chickens[J]. Frontiers in Veterinary Science,2022,9:959449. doi: 10.3389/fvets.2022.959449
[39]	唐茹萌, 焦文雅, 桑亚新, 等. 裙带菜多糖体外和体内降血脂活性[J]. 食品科学,2022,43(1):142−149. [TANG R M, JIAO W Y, SANG Y X, et al. In vitro and in vivo hypolipidemic effect of Undaria pinnatifida polysaccharide[J]. Food Science,2022,43(1):142−149.] TANG R M, JIAO W Y, SANG Y X, et al. In vitro and in vivo hypolipidemic effect of Undaria pinnatifida polysaccharide[J]. Food Science, 2022, 43（1）: 142−149.
[40]	陈远菲, 严德林, 莫雪婷, 等. 甘蔗叶多糖提取工艺优化及生物活性研究[J]. 食品研究与开发,2022,43(19):155−165. [CHEN Y F, YAN D L, MO X T, et al. Optimization of extraction process of polysaccharides from sugarcane leaves and characterization of biological activities[J]. Food Research and Development,2022,43(19):155−165.] CHEN Y F, YAN D L, MO X T, et al. Optimization of extraction process of polysaccharides from sugarcane leaves and characterization of biological activities[J]. Food Research and Development, 2022, 43（19）: 155−165.
[41]	NGUYEN T P, PHAN H N, DO T D, et al. Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata:Antioxidant, wound-healing, antibacterial, and cytotoxic activities[J]. Heliyon,2023,9(3):e13559. doi: 10.1016/j.heliyon.2023.e13559

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(9)

1.	陈金足，韦晓雯，农晶晶，韩丽芳，冯学，唐婷范，李利军，程昊. 氢氧化镁-活性炭复合材料的制备及其对糖浆脱色工艺优化. 食品工业科技. 2025(01): 201-207 . 本站查看
2.	郑婷婷，吕建彪，龚婉莹，王礼中，张文杰，严亮. 白及叶多糖脱色脱蛋白质方法及其抗氧化活性研究. 粮食与油脂. 2024(03): 86-90+105 .
3.	杨紫焰，李自霖，张翠香，黄丽金，陈贵元，李雪英. 响应面法优选穿心莲多糖大孔树脂脱色工艺及其抗氧化活性研究. 安徽农学通报. 2024(10): 89-95 .
4.	潘金涛，沈晓岩，陈亮，武小芬，齐慧，刘安，魏东宁，邓明. 油茶壳低聚木糖水热处理液脱色条件优化. 湖南农业科学. 2024(11): 76-80+95 .
5.	赵玉荣，许金玉，侯宪邦，陆姗姗. 酶解法提取夏枯草中多糖的工艺研究. 药品评价. 2024(08): 946-950 .
6.	郑艳宇，王平，刘思冶，郝明洋，赵思远，薛晓丽. 黄精多糖的树脂法脱色. 吉林化工学院学报. 2024(09): 41-46 .
7.	黄丽金，李自霖，陈贵元. 响应面法优化苦胆草多糖脱色工艺. 安徽农学通报. 2023(09): 157-160+170 .
8.	黄丽金，陈贵元. 苦胆草多糖活性炭脱色工艺研究. 安徽农学通报. 2023(13): 32-36 .
9.	李自霖，陈贵元. 中药多糖提取物脱色工艺研究进展. 安徽农学通报. 2023(13): 37-40 .