Effect of Gradient Heating Method on the Microbial Changes and Flavor Formation of Liquid-State Soy Sauce Fermented with Different Salt Concentrations

LI Xinzhi; LIU Xi; XU Xinyu; ZHOU Qiyang; WU Changzheng; TONG Xing; OU Shiyi

doi:10.13386/j.issn1002-0306.2023080043

Volume 45 Issue 9

Apr. 2024

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Science and Technology of Food Industry > 2024 > 45(9): 106-114. > DOI: 10.13386/j.issn1002-0306.2023080043

LI Xinzhi, LIU Xi, XU Xinyu, et al. Effect of Gradient Heating Method on the Microbial Changes and Flavor Formation of Liquid-State Soy Sauce Fermented with Different Salt Concentrations[J]. Science and Technology of Food Industry, 2024, 45(9): 106−114. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080043.

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Effect of Gradient Heating Method on the Microbial Changes and Flavor Formation of Liquid-State Soy Sauce Fermented with Different Salt Concentrations

LI Xinzhi^{1, 2,},
LIU Xi³,
XU Xinyu⁴,
ZHOU Qiyang^{3, 5},
WU Changzheng^{4, 6},
TONG Xing^{3, 5, ,},
OU Shiyi^1, ,

1.
Department of Food Science and Technology, Jinan University, Guangzhou 510632, China
2.
Foshan Haitian Flavoring & Food Co., Ltd., Foshan 528000, China
3.
Foshan Haitian (Gaoming) Flavoring & Food Co., Ltd., Foshan 528511, China
4.
Guangdong Haitian Innovation Technology Co., Ltd., Foshan 528000, China
5.
Key Laboratory of Advanced Technology Enterprise of Guangdong Seasoning Food Biofermentation, Foshan 528000, China
6.
Guangdong Provincial Research Centre of Brewing Microbiology Breeding and Fermentation Engineering Technology, Foshan 528000, China

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Received Date: August 05, 2023
Available Online: March 02, 2024

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Abstract

Abstract

In this paper, liquid-state fermented sauce mash with salt concentrations of 12% and 18% (w/v) was used as the research object, to explore the effect of gradient heating (15 ℃-20 ℃-25 ℃-30 ℃) on the microbial counting and flavor formation of fermented sauce mash. The results showed that the yeast counts of the low-salt sauce mash sample (TC12) using the gradient heating method was about 1.0 log CFU/g higher than that of the constant temperature (30 ℃) control group (C12) at the end of fermentation, which indicated that the yeasts maintained growth and metabolism for a long time in the gradient heating group. This might be related to the fact that the lower temperature in the early stage of fermentation limited the rapid propagation of yeasts. Among them, the amino nitrogen of the fermented soy sauce from TC12 was as high as 1.14 g/100 g, which met the standard of premium-grade brewed soy sauce in China. At the same time, the content of umami (19.03 g/L) and sweet amino acids (19.32 g/L) in TC12 was high, accounting for about 60% of the total amino acids, presenting a better sweet and savory taste. The volatile compound detection data showed that the content of ethanol, ethyl phenylacetate, ethyl palmitate, 3-(methylthiopropionaldehyde) and benzaldehyde in TC12 was relatively high, and the sample showed an overall sweet-fruity aroma and ester-like aroma. On the other hand, the gradient heating sample of the high-salt group (TC18) contained more guaiacol and methyl maltol, showing a smoky incense and saucy aroma. The sensory evaluation results showed that the low-salt gradient heating fermentation samples TC12 had higher aroma and taste scores, as well as better comprehensive tastes than the constant temperature fermentation samples C12. In summary, the gradient heating method is beneficial to limit the excessive growth of yeast in low-salt (12%) fermented sauce mash in the early stage and maintaining a considerable cell count of yeast in the late stage of fermentation, so that the yeast can continuously ferment and generate more flavor substances and improve the overall flavor of low-salt fermented soy sauce.
- gradient heating method,
- low-salt fermented soy sauce,
- yeast,
- amino nitrogen,
- free amino acids,
- volatile flavor compounds

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References (31)

References

[1]	DEVANTHI P V P, GKATZIONIS K. Soy sauce fermentation:Microorganisms, aroma formation, and process modification[J]. Food Research International,2019,120:364−374. doi: 10.1016/j.foodres.2019.03.010
[2]	WAH T T, WALAISRI S, ASSAVANIG A, et al. Co-culturing of Pichia guilliermondii enhanced volatile flavor compound formation by Zygosaccharomyces rouxii in the model system of Thai soy sauce fermentation[J]. International Journal of Food Microbiology,2013,160(3):282−289. doi: 10.1016/j.ijfoodmicro.2012.10.022
[3]	刘贺, 章启鹏, 徐婧婷, 等. 减盐相关产品研究进展及开发现状[J]. 中国调味品,2017,42(11):175−180. [LIU H, HE Q Z, XU Q T, et al. Research progress and development status of low-salt products[J]. China Condiment,2017,42(11):175−180.] LIU H, HE Q Z, XU Q T, et al. Research progress and development status of low-salt products[J]. China Condiment, 2017, 42（11）: 175−180.
[4]	SHAO S, HUA Y, YANG Y, et al. Salt reduction in China:A state-of-the-art review[J]. Risk Management and Healthcare Policy, 2017:17−28.
[5]	LIN X, TANG Y, HU Y, et al. Sodium reduction in traditional fermented foods:Challenges, strategies, and perspectives[J]. Journal of Agricultural and Food Chemistry,2021,69(29):8065−8080. doi: 10.1021/acs.jafc.1c01687
[6]	尹文颖, 崔春, 陈玲, 等. 发酵温度对高盐稀态酱油原油品质的影响[J]. 食品工业科技,2014,35(2):154−157. [YIN W Y, CUI C, CHEN L, et al. Effects of fermentation temperature on the quality of soy sauce[J]. Science and Technology of Food Industry,2014,35(2):154−157.] YIN W Y, CUI C, CHEN L, et al. Effects of fermentation temperature on the quality of soy sauce[J]. Science and Technology of Food Industry, 2014, 35（2）: 154−157.
[7]	于茜雅, 鲁骞, 吴昌正, 等. 温度对广式高盐稀态酱油原油品质的影响[J]. 食品科学,2023,44(22):55−63. [YU X Y, LU Q, WU C Z, et al. Effect of ambient temperature on the quality of high-salt liquid Cantonese raw soy sauce[J]. Food Science,2023,44(22):55−63.] YU X Y, LU Q, WU C Z, et al. Effect of ambient temperature on the quality of high-salt liquid Cantonese raw soy sauce[J]. Food Science, 2023, 44（22）: 55−63.
[8]	HOANG N X, FERNG S, TING C H, et al. Optimizing the initial moromi fermentation conditions to improve the quality of soy sauce[J]. LWT-Food Science and Technology,2016,74:242−250. doi: 10.1016/j.lwt.2016.07.049
[9]	国家卫生和计划生育委员会. 食品安全国家标准酱油中还原糖的测定:GB 5009.7-2016[S]. 北京:中国标准出版社, 2016. [National Health and Family Planning Commission. National Standard of Food Safety-Determination of reducing sugar in soy sauce:GB 5009.7-2016[S]. Beijing:China Standards Press, 2016.] National Health and Family Planning Commission. National Standard of Food Safety-Determination of reducing sugar in soy sauce: GB 5009.7-2016[S]. Beijing: China Standards Press, 2016.
[10]	国家卫生和计划生育委员会. 食品安全国家标准食品中氨基酸态氮的测定:GB 5009.235-2016[S]. 北京:中国标准出版社, 2016. [National Health and Family Planning Commission. National Standard of Food Safety-Determination of amino nitrogen in foods:GB 5009.235-2016[S]. Beijing:China Standards Press, 2016.] National Health and Family Planning Commission. National Standard of Food Safety-Determination of amino nitrogen in foods: GB 5009.235-2016[S]. Beijing: China Standards Press, 2016.
[11]	国家卫生和计划生育委员会. 食品安全国家标准植物中游离氨基酸的测定:GB/T 30987-2020[S]. 北京:中国标准出版社, 2020. [National Health and Family Planning Commission. National Standard of Food Safety-Determination of free amino acids in plants:GB/T 30987-2020[S]. Beijing:China Standards Press, 2020.] National Health and Family Planning Commission. National Standard of Food Safety-Determination of free amino acids in plants: GB/T 30987-2020[S]. Beijing: China Standards Press, 2020.
[12]	阮志强, 董玺梅, 蒋雪薇, 等. 高盐稀态酱油发酵优势真菌与风味物质相关性分析[J]. 食品科学,2022,43(10):172−179. [RUAN Z Q, DONG X M, JIANG X W, et al. Study on the correlation between dominant fungi and the variation of flavor compounds in high-salt liquid-state soy sauce fermentation[J]. Food Science,2022,43(10):172−179.] RUAN Z Q, DONG X M, JIANG X W, et al. Study on the correlation between dominant fungi and the variation of flavor compounds in high-salt liquid-state soy sauce fermentation[J]. Food Science, 2022, 43（10）: 172−179.
[13]	苏国万, 赵炫, 张佳男, 等. 酱油中鲜味二肽的分离鉴定及其呈味特性研究[J]. 现代食品科技,2019,35(5):7−15. [SU G W, ZHAO X, ZHANG J N, et al. Isolation, identification and taste characteristics of umami dipeptides from soy sauce[J]. Modern Food Science and Technology,2019,35(5):7−15.] SU G W, ZHAO X, ZHANG J N, et al. Isolation, identification and taste characteristics of umami dipeptides from soy sauce[J]. Modern Food Science and Technology, 2019, 35（5）: 7−15.
[14]	ELHALIS H, CHIN X H, CHOW Y. Soybean fermentation:Microbial ecology and starter culture technology[J]. Critical Reviews in Food Science and Nutrition, 2023:1-23.
[15]	WEI C L, CHAO S H, TSAI W B, et al. Analysis of bacterial diversity during the fermentation of Inyu, a high-temperature fermented soy sauce, using nested PCR-denaturing gradient gel electrophoresis and the plate count method[J]. Food Microbiology,2013,33(2):252−261. doi: 10.1016/j.fm.2012.10.001
[16]	WANG J, ZHAO M, XIE N, et al. Community structure of yeast in fermented soy sauce and screening of functional yeast with potential to enhance the soy sauce flavor[J]. International Journal of Food Microbiology,2022,370:109652. doi: 10.1016/j.ijfoodmicro.2022.109652
[17]	HARADA R, YUZUKI M, ITO K, et al. Influence of yeast and lactic acid bacterium on the constituent profile of soy sauce during fermentation[J]. Journal of Bioscience and Bioengineering,2017,123(2):203−208. doi: 10.1016/j.jbiosc.2016.08.010
[18]	SONG Y R, JEONG D Y, BAIK S H. Effects of indigenous yeasts on physicochemical and microbial properties of Korean soy sauce prepared by low-salt fermentation[J]. Food Microbiology,2015,51:171−178. doi: 10.1016/j.fm.2015.06.001
[19]	SINGRACHA P, NIAMSIRI N, VISESSANGUAN W, et al. Application of lactic acid bacteria and yeasts as starter cultures for reduced-salt soy sauce (moromi) fermentation[J]. LWT-Food Science and Technology,2017,78:181−188. doi: 10.1016/j.lwt.2016.12.019
[20]	ZHANG L, XIONG S, DU T, et al. Effect of microbiota succession on the dynamics of characteristic flavors and physicochemical properties during the soy sauce fermentation[J]. Food Bioscience, 2023:102883.
[21]	LIU X, QIAN M, SHEN Y, et al. An high-throughput sequencing approach to the preliminary analysis of bacterial communities associated with changes in amino acid nitrogen, organic acid and reducing sugar contents during soy sauce fermentation[J]. Food Chemistry,2021,349:129131. doi: 10.1016/j.foodchem.2021.129131
[22]	LIU B, LI Y, CAO Z, et al. Effect of Tetragenococcus halophilus, Zygosaccharomyces rouxii, and Torulopsis versatilis addition sequence on soy sauce fermentation[J]. Innovative Food Science & Emerging Technologies,2021,69:102662.
[23]	梁寒峭, 陈建国, 刘伟, 等. 酿造酱油中特征氨基酸含量检测及对氨基酸态氮贡献的分析[J]. 食品与发酵工业,2018,44(4):198−203. [LIANG H Q, CHEN J G, LIU W, et al. Analysis of amino acids content and contribution to amino nitrogen in fermented soy sauce[J]. Food and Fermentation Industries,2018,44(4):198−203.] LIANG H Q, CHEN J G, LIU W, et al. Analysis of amino acids content and contribution to amino nitrogen in fermented soy sauce[J]. Food and Fermentation Industries, 2018, 44（4）: 198−203.
[24]	国家质量技术监督局. 食品安全国家标准:GB/T 18186-2000 酿造酱油[S]. 北京:中国标准出版社, 2000. [State Bureau of Quality and Technical Supervision. National Standard of Food Safety:GB/T 18186-2000 Brewed soy sauce[S]. Beijing:China Standards Press, 2000.] State Bureau of Quality and Technical Supervision. National Standard of Food Safety: GB/T 18186-2000 Brewed soy sauce[S]. Beijing: China Standards Press, 2000.
[25]	KUANG X, SU H, LI W, et al. Effects of microbial community structure and its co-occurrence on the dynamic changes of physicochemical properties and free amino acids in the Cantonese soy sauce fermentation process[J]. Food Research International,2022,156:111347. doi: 10.1016/j.foodres.2022.111347
[26]	HAN D M, CHUN B H, FENG T, et al. Dynamics of microbial communities and metabolites in Ganjang, a traditional Korean fermented soy sauce, during fermentation[J]. Food Microbiology,2020,92:103591. doi: 10.1016/j.fm.2020.103591
[27]	ZHOU T, FENG Y, CHEN Y, et al. Quantitative studies, taste recombination, and omission experiments on the key taste compounds in Chinese and Japanese soy sauce[J]. Food Chemistry,2023,403:134215. doi: 10.1016/j.foodchem.2022.134215
[28]	ZHANG W, HAN Y, SHI K, et al. Effect of different sulfur-containing compounds on the structure, sensory properties and antioxidant activities of Maillard reaction products obtained from Pleurotus citrinopileatus hydrolysates[J]. LWT-Food Science and Technology,2022,171:114144. doi: 10.1016/j.lwt.2022.114144
[29]	HU G, CHEN J, DU G, et al. Moromi mash dysbiosis trigged by salt reduction is relevant to quality and aroma changes of soy sauce[J]. Food Chemistry,2023,406:135064. doi: 10.1016/j.foodchem.2022.135064
[30]	ZHANG L, ZHANG L, XU Y. Effects of Tetragenococcus halophilus and Candida versatilis on the production of aroma-active and umami-taste compounds during soy sauce fermentation[J]. Journal of the Science of Food and Agriculture,2020,100(6):2782−2790. doi: 10.1002/jsfa.10310
[31]	LIU D, HE Y, XIAO J, et al. The occurrence and stability of Maillard reaction products in various traditional Chinese sauces[J]. Food Chemistry,2021,342:128319. doi: 10.1016/j.foodchem.2020.128319

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