Optimization of Ultrasound-assisted Extraction of Phytoglycogen from Sweet Corn and Its Bioactivity Evaluation

CHEN Qiang; WANG Lu; XU Zhengrong; LUO Jinchao; DENG Qianqian; FANG Yuting; LI Conghu; CHENG Xu

doi:10.13386/j.issn1002-0306.2023070133

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 177-186. > DOI: 10.13386/j.issn1002-0306.2023070133

CHEN Qiang, WANG Lu, XU Zhengrong, et al. Optimization of Ultrasound-assisted Extraction of Phytoglycogen from Sweet Corn and Its Bioactivity Evaluation[J]. Science and Technology of Food Industry, 2024, 45(19): 177−186. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070133.

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Optimization of Ultrasound-assisted Extraction of Phytoglycogen from Sweet Corn and Its Bioactivity Evaluation

CHEN Qiang^1,,
WANG Lu¹,
XU Zhengrong¹,
LUO Jinchao¹,
DENG Qianqian¹,
FANG Yuting¹,
LI Conghu^{1, 2, ,},
CHENG Xu^{1, 2, ,}

1.
College of Life Science, Anqing Normal University, Anqing 246133, China
2.
The Key Laboratory of Biodiversity Conservation and Characteristic Resources Utilization in Southwest Anhui Province, Anqing 246133, China

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Received Date: July 16, 2023
Available Online: August 04, 2024

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Abstract

Abstract

The optimal extraction process of phytoglycogen (PG) from sweet corn by ultrasound-assisted method was studied, and the physicochemical properties and biological activities of PG were evaluated in this paper. The effects of solid-liquid ratio, ultrasonic power and ultrasonic time on the yield of PG were investigated and the process parameters were further optimized by orthogonal experiment. Furthermore, the physicochemical properties of PG obtained by ultrasound-assisted method were investigated. Meanwhile, the antioxidation, cytotoxicity, apoptosis and hemolysis of PG were evaluated in vitro. The results showed that the optimal extraction conditions were as follows: Solid-liquid ratio of 1:9 (g/mL), ultrasonic power of 160 W and ultrasonic time of 80 min, the actual yield was 14.31%±0.38%. The PG particle size obtained by ultrasound-assisted method was lower than that obtained by traditional water extraction and alcohol precipitation method. In vitro antioxidant experiment showed that PG had a certain antioxidant capacity, especially it was more sensitive to DPPH free radicals, and the clearance rate reached 54.58%±1.39% at 20 mg/mL. The results of biosafety evaluation showed that the survival rate of mouse fibroblasts (3T3) was higher than 95% after incubation with different concentrations of PG for 24 or 48 h. Meanwhile, PG had no obvious apoptotic effect on cells, and the apoptotic rate was lower than 7% at the maximum concentration. After 2 h of co-incubation, the hemolysis rate caused by PG on red blood cells was also much lower than the national standard (5%), which confirmed that PG had good biocompatibility with normal cells and red blood cells. Overall, this study showed that ultrasound-assisted method could improve the yield and physicochemical properties of PG, and also confirmed that PG was a non-toxic natural nanoparticle, which had great application potential in drug or food factor delivery.
- phytoglycogen,
- ultrasound-assisted extraction,
- biological stability,
- biological safety

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