Optimization of Ultrasonic-Enzyme-Assisted Deep Eutectic Solvents Extraction Process of Total Flavonoids from Mulberry Leaves and Its Antioxidant Activity

WU Jun; WU Junting; YANG Biwen; WANG Mei; ZHAO Pei; MA Jingqiu; HUANG Yue; HUANG Chuanshu

doi:10.13386/j.issn1002-0306.2023070033

Volume 45 Issue 3

Jan. 2024

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Science and Technology of Food Industry > 2024 > 45(3): 31-39. > DOI: 10.13386/j.issn1002-0306.2023070033

WU Jun, WU Junting, YANG Biwen, et al. Optimization of Ultrasonic-Enzyme-Assisted Deep Eutectic Solvents Extraction Process of Total Flavonoids from Mulberry Leaves and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2024, 45(3): 31−39. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070033.

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Optimization of Ultrasonic-Enzyme-Assisted Deep Eutectic Solvents Extraction Process of Total Flavonoids from Mulberry Leaves and Its Antioxidant Activity

1.
Chongqing Sericulture Science and Technology Research Institute, Chongqing 400700, China
2.
Army Logistics University of PLA, Chongqing 401331, China

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Received Date: July 05, 2023

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Abstract

This study used ultrasound-enzyme-assisted deep eutectic solvents to establish a green and highly efficient total flavonoids extraction process from mulberry leaves. On the basis of the results of single-factor experiments, the extraction amount of total flavonoids was used as the response value, and the Box-Behnken response surface method was used to optimize, and the antioxidative ability of total flavonoids from mulberry leaves on DPPH radicals and ABTS⁺ radicals was studied. The results showed that when the choline chloride/fructose/alcohol molar ratio was 1:1:3, water content 30%, liquid-solid ratio 40 mL/g, ultrasonic power 360 W, ultrasonic temperature 40 ℃, ultrasonic time 40 min, dosage of enzyme 4%, the extraction amount of total flavonoids from mulberry leaves reached 46.58 mg/g. When the mass concentration of total flavonoids from mulberry leaves was 0.08 mg/mL, the scavenging rate of DPPH radicals was 98.36%. And when the mass concentration of total flavonoids from mulberry leaves was 0.2 mg/mL, the scavenging rate of ABTS⁺ radicals was 72.12%. Therefore, the ultrasonic-enzyme-assisted deep eutectic solvents method was green and simple to operate, and could effectively improve the extraction yield of total flavonoids from mulberry leaves. This method provided a scientific basis for the development and utilization of mulberry leaf resources.

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