Whole Genome Sequencing and Biological Characterization of <i>Bacillus cereus</i> GW-01

LIU Shan; JIANG Yangdan; YAN Jisha; XIE Yuxuan; ZHAO Sijia; HE Qitian; ZHAO Jiayuan

doi:10.13386/j.issn1002-0306.2023060031

Volume 45 Issue 7

Mar. 2024

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Science and Technology of Food Industry > 2024 > 45(7): 167-176. > DOI: 10.13386/j.issn1002-0306.2023060031

LIU Shan, JIANG Yangdan, YAN Jisha, et al. Whole Genome Sequencing and Biological Characterization of Bacillus cereus GW-01[J]. Science and Technology of Food Industry, 2024, 45(7): 167−176. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060031.

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Whole Genome Sequencing and Biological Characterization of Bacillus cereus GW-01

LIU Shan^{1, 2,},
JIANG Yangdan^{1, 2},
YAN Jisha^{1, 2},
XIE Yuxuan^{1, 2},
ZHAO Sijia^{1, 2},
HE Qitian³,
ZHAO Jiayuan^{1, 2, ,}

1.
Key Laboratory of Land Resources Evaluation and Monitoring in Southwest (Sichuan Normal University), Ministry of Education, Chengdu 610101, China
2.
College of Life Science, Sichuan Normal University, Chengdu 610101, China
3.
School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310000, China

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Received Date: June 05, 2023
Available Online: January 30, 2024

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Abstract

Abstract

Objective: To analyze the genome sequence information and biological properties of the pre-screened high-performance cypermethrin (β-CY)-degrading Bacillus cereus strain GW-01 through whole genome sequencing and bioinformatics, and to provide valuable insights for its safety assessment. Methods: The degradation ability of GW-01 towards β-CY was verified using HPLC. The whole genome of strain GW-01 was sequenced using third-generation single-molecule sequencing technology based on the second-generation Illumina NovaSeq and third-generation PacBio Sequel sequencing platforms. The sequencing data underwent analyses for genome assembly, gene prediction, functional annotation, prediction of carbohydrate-activating enzymes, virulence factors, and antibiotic resistance. Additionally, a phylogenetic tree was constructed for strain GW-01 based on the gyrA gene sequence. Results: On day 6, GW-01 degraded 48.4% of β-CY. The whole genome size of GW-01 strain was 5244145 bp with a cyclic genome type and an average GC content of 36.43%. It encoded a total of 5314 genes, including 104 tRNA genes and 313 ncRNAs. The whole genome sequences of GW-01 were annotated in the GO, KEGG, COG, NR, Swiss-Prot, CAZy, VFDB, and CARD databases, yielding 3536, 4714, 4434, 5290, 3854, 135, 263 and 96 annotated genes, respectively. GW-01 exhibited higher GC content and fewer virulence factors and resistance genes compared to the other four closely related strains. Conclusion: GW-01 displayed a distinct evolutionary pathway and a lower number of toxin-encoding genes compared to common Bacillus cereus strains, suggesting its higher safety profile.
- Bacillus cereus,
- whole genome sequencing,
- biological characteristics,
- safety

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References (54)

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