Advances in the Rapid Detection of Foodborne Pathogens by Nucleic Acid Amplification Lateral Flow Assays

YUN Ziguang; WEI Yong; ZHANG Jian; CUI Shuangshuang; LI Can; SUN Fengxia

doi:10.13386/j.issn1002-0306.2023050216

Volume 45 Issue 5

Feb. 2024

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Science and Technology of Food Industry > 2024 > 45(5): 412-425. > DOI: 10.13386/j.issn1002-0306.2023050216

YUN Ziguang, WEI Yong, ZHANG Jian, et al. Advances in the Rapid Detection of Foodborne Pathogens by Nucleic Acid Amplification Lateral Flow Assays[J]. Science and Technology of Food Industry, 2024, 45(5): 412−425. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050216.

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Advances in the Rapid Detection of Foodborne Pathogens by Nucleic Acid Amplification Lateral Flow Assays

YUN Ziguang^{1, 2,},
WEI Yong³,
ZHANG Jian^{1, 2},
CUI Shuangshuang^{1, 2},
LI Can^{1, 2},
SUN Fengxia^{1, 2, 3, ,}

1.
Key Laboratory of Agricultural Product Processing and Quality Control of Specialty (Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi 832000, China
2.
Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi 832000, China
3.
Xinjiang Tianrun Dairy Co., Ltd., Urumqi 830063, China

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Received Date: May 17, 2023
Available Online: January 02, 2024

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Abstract

Abstract

Foodborne pathogens remain a serious threat to food safety, while traditional assays are time-consuming and labor-intensive, and difficult to match with the rapid and simple requirements of modern food safety detection. Molecular biology-based assays, such as polymerase chain reaction, isothermal nucleic acid amplification, and clustered regularly interspaced short palindromic repeats-based nucleic acid detection technology, have been widely employed in the detection of foodborne pathogens, and play an important role in ensuring food safety. In recent years, the combination of nucleic acid amplification detection technology and immunochromatography technology has become a hotspot for rapidly detecting foodborne pathogens. Here, the research progress in the rapid detection of foodborne pathogens are summarized by combining nucleic acid amplification and lateral flow immunoassays, highlight their advantages and disadvantages, and provide a comprehensive outlook on this field. This review can provide a reference for detecting, preventing, and controlling foodborne pathogens.
- foodborne pathogens,
- nucleic acid amplification,
- lateral flow strip,
- rapid detection

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References

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