Preliminary Study on the Mechanism of Chitosan and <i>ε</i>-Polylysine Inhibition against <i>Carbibacterium divergens</i>

HUANG Weiying; LIU Xiaoting; GUAN Yufeng; WU Yihui; FAN Canye; LIU Qiaoyu; CHEN Haiguang; WU Junshi; WANG Xiaoyu

doi:10.13386/j.issn1002-0306.2023050105

Volume 45 Issue 5

Feb. 2024

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Science and Technology of Food Industry > 2024 > 45(5): 144-152. > DOI: 10.13386/j.issn1002-0306.2023050105

HUANG Weiying, LIU Xiaoting, GUAN Yufeng, et al. Preliminary Study on the Mechanism of Chitosan and ε-Polylysine Inhibition against Carbibacterium divergens[J]. Science and Technology of Food Industry, 2024, 45(5): 144−152. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050105.

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Preliminary Study on the Mechanism of Chitosan and ε-Polylysine Inhibition against Carbibacterium divergens

1.
College of Light Industry and Food Sciences, Guangdong Key Laboratory of Science and Technology of Lingnan Special Food, Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Innovation Research Institute of Modern Agricultural Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
2.
Guangzhou Furui High Energy Technology Co., Ltd., Guangdong Industrial Co-60 Gamma Ray Application Engineering Technology Research Center, Guangzhou 511458, China
3.
Technical Center of Gongbei Customs, Zhuhai 519015, China

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Received Date: May 09, 2023
Available Online: December 27, 2023

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Abstract

Abstract

To investigate the effects of chitosan and ε-polylysine on the cell structure and cytoprotective enzymes of Carbibacterium divergens to explore their inhibition mechanism against Carbibacterium divergens, the broth dilution method was used to determine the minimum inhibitory concentrations (MICs) of chitosan and ε-polylysine against Carbibacterium divergens. Their effects on the cellular structure of Carbibacterium divergens were then assessed by several indexes, such as growth curves, electronic conductivity, extracellular nucleic acids, extracellular proteins, and alkaline phosphatase (AKP) activity, and the microstructure of bacteria were also observed by scanning electron microscopy (SEM). In addition, their role in oxidative stress was analyzed by detecting changes in catalase (CAT) activity and superoxide dismutase (SOD) activity. The results showed that the MIC of chitosan and ε-polylysine against Carbibacterium divergens was 0.1953 and 0.1563 mg/mL, respectively. The intracellular components (AKP, proteins, nucleic acids, and ions) leaked after treatment with MIC and 2MIC chitosan and ε-polylysine. The mass concentrations of chitosan and ε-polylysine were negatively correlated with enzyme activity, and the activities CAT and SOD significantly decreased (P<0.05). Scanning electron microscopy showed that chitosan could deform the cell and its contents oozed out, while ε-polylysine caused the cell to fold and distort, forming holes on the cell surface. In summary, both chitosan and ε-polylysine can inhibit the growth of Carbibacterium divergens by destroying cell wall, damaging cell membrane and generating oxidative stress reaction.
- chitosan,
- ε-polylysine,
- Carbibacterium divergens,
- cell structure,
- antibacterial

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References (42)

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