WU Shuanghui, YANG Ziyao, NIU Yin, et al. Effects of Mixed Starter Culture on Quality and Volatile Flavor of Fermented Sausages[J]. Science and Technology of Food Industry, 2024, 45(5): 126−133. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040273.
Citation: WU Shuanghui, YANG Ziyao, NIU Yin, et al. Effects of Mixed Starter Culture on Quality and Volatile Flavor of Fermented Sausages[J]. Science and Technology of Food Industry, 2024, 45(5): 126−133. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040273.

Effects of Mixed Starter Culture on Quality and Volatile Flavor of Fermented Sausages

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  • Received Date: April 27, 2023
  • Available Online: December 28, 2023
  • In this study, headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) and other techniques were used to analyze the regular physical-chemical and microbiological properties, the content and aroma pattern of main volatile flavors, and the sensory evaluation of fermented sausages made with the inoculation of the mixed culture Lactobacillus plantarum L77 and Staphylococcus xylosus S120 (L77-S120) and the individual culture Lactobacillus plantarum 77 (L77), with the non-inoculated group as blank. The results showed that the TBARS value, nitrite content and Enterobacteriaceae count of the L77-S120 group were significantly lower than those of the blank and L77 group (P<0.05). The L77-S120 group had the highest number and content of major volatile flavor compounds, of which there were 10 aldehydes, 3 alcohols, 2 esters, 1 acid and 4 others. The differential aroma compounds for the L77-S120 group included heptanal, (E)-2-heptenal, mushroom alcohol, 2-pentyl-furan, trans-2-octenal, trans-2,4-decadienal, trans-2-undecanonal, 2-ethyl-butyric acid, ethyl decanoate, pentanal and nonanol, and these main volatile flavor compounds had the most potential in the production of oily, creamy, floral and fruity aromas. In combination with the results of sensory evaluation, the mixed culture of Lactobacillus plantarum L77 and Staphylococcus xylosus S120 could be as a flavor-producing starter culture for the production of fermented sausages.
  • [1]
    CASABURI A, ARISTOY M C, CAVELLA S, et al. Biochemical and sensory characteristics of traditional fermented sausages of Vallo di Diano (Southern Italy) as affected by the use of starter cultures[J]. Meat Science,2007,76(2):295−307. doi: 10.1016/j.meatsci.2006.11.011
    [2]
    李玉娥, 李晓宏, 朱迎春, 等. 商业发酵剂对发酵香肠成熟过程中理化性质的影响[J]. 肉类研究,2019,33(8):42−47. [LI Y E, LI X H, ZHU Y C, et al. Effects of commercial starter cultures on physicochemical properties of fermented sausage during ripening[J]. Meat Research,2019,33(8):42−47.]

    LI Y E, LI X H, ZHU Y C, et al. Effects of commercial starter cultures on physicochemical properties of fermented sausage during ripening[J]. Meat Research, 2019, 338): 4247.
    [3]
    李月明, 张根生. 乳酸菌在发酵肉制品中的应用研究进展[J]. 肉类研究,2022,36(10):51−56. [LI Y M, ZHANG G S. Recent progress on the application of lactic acid bacteria in fermented meat products[J]. Meat Research,2022,36(10):51−56.]

    LI Y M, ZHANG G S. Recent progress on the application of lactic acid bacteria in fermented meat products[J]. Meat Research, 2022, 3610): 5156.
    [4]
    STAHNKE L H. Dried sausages fermented with Staphylococcus xylosus at different temperatures and with different ingredient levels-Part L chemical and bacteriological data[J]. Meat Science,1995,41(2):179−191. doi: 10.1016/0309-1740(94)00070-N
    [5]
    王恺, 慕妮, 李亮, 等. 不同发酵剂对发酵香肠挥发性风味物质的影响[J]. 食品工业科技,2013(14):177−181,196. [WANG K, MU N, LI L, et al. Analysis of aroma components in fermented sausage with different starter cultures[J]. Science and Technology of Food Industry,2013(14):177−181,196.]

    WANG K, MU N, LI L, et al. Analysis of aroma components in fermented sausage with different starter cultures[J]. Science and Technology of Food Industry, 201314): 177181,196.
    [6]
    FLORES M, PIORNOS J A. Fermented meat sausages and the challenge of their plant-based alternatives:A comparative review on aroma-related aspects[J]. Meat Science,2021,182(1):108636.
    [7]
    TALON R, ZAGOREC M. Beneficial microorganisms for food manufacturing-fermented and bio-preserved foods and beverages[J]. Microorganisms,2017,5(4):71−79. doi: 10.3390/microorganisms5040071
    [8]
    GE Q, PEI H, LIU R, et al. Effects of Lactobacillus plantarum NJAU-01 from Jinhua ham on the quality of dry-cured fermented sausage[J]. LWT,2019,101:513−518. doi: 10.1016/j.lwt.2018.11.081
    [9]
    WANG J, HOU J, ZHANG X, et al. Improving the flavor of fermented Sausage by increasing its bacterial quality via inoculation with Lactobacillus plantarum MSZ2 and Staphylococcus xylosus YCC3[J]. Foods,2022,11(5):736. doi: 10.3390/foods11050736
    [10]
    XIAO Y, LIU Y, CHEN C, et al. Effect of Lactobacillus plantarum and Staphylococcus xylosus on flavour development and bacterial communities in Chinese dry fermented sausages[J]. Food Research International,2020,135:109247. doi: 10.1016/j.foodres.2020.109247
    [11]
    蔡永敏, 黄海英, 胡炜东, 等. 混合发酵剂的筛选及对发酵猪肉香肠加工特性及安全性能的影响[J]. 食品科技,2022(1):138−144. [CAI Y M, HUANG H Y, HU W D, et al. Screening of mixed starter and its effect on processing characteristics and safety of fermented pork sausage[J]. Food Science and Technology,2022(1):138−144.]

    CAI Y M, HUANG H Y, HU W D, et al. Screening of mixed starter and its effect on processing characteristics and safety of fermented pork sausage[J]. Food Science and Technology, 20221): 138144.
    [12]
    CHEN X, MI R, QI B, et al. Effect of proteolytic starter culture isolated from Chinese Dong fermented pork (Nanx Wudl) on microbiological, biochemical and organoleptic attributes in dry fermented sausages[J]. Food Science and Human Wellness,2021,10(1):13−22.
    [13]
    刘浩, 吴叶, 张建萍, 等. 混合菌辅助发酵羊肉香肠研制及产品质量分析[J]. 中国调味品,2021(9):69−73. [LIU H, WU Y, ZHANG J P, et al. Development and product quality analysis of mutton sausage assisted fermented by mixed bacteria[J]. China Condiment,2021(9):69−73.]

    LIU H, WU Y, ZHANG J P, et al. Development and product quality analysis of mutton sausage assisted fermented by mixed bacteria[J]. China Condiment, 20219): 6973.
    [14]
    韩云飞, 翟钰佳, 郭骏飞, 等. 复配发酵剂对羊肉发酵香肠脂肪氧化及脂肪酸组成的影响[J]. 食品与发酵工业,2019(11):99−105. [HAN Y F, ZHAI Y J, GUO J F, et al. Effects of compound starter on fat oxidation and fatty acid composition of mutton fermented sausage[J]. Food and Fermentation Industries,2019(11):99−105.]

    HAN Y F, ZHAI Y J, GUO J F, et al. Effects of compound starter on fat oxidation and fatty acid composition of mutton fermented sausage[J]. Food and Fermentation Industries, 201911): 99105.
    [15]
    MEJRI L, ZIADI A, EL ADAB S, et al. Effect of commercial starter cultures on physicochemical, microbiological and textural characteristics of a traditional dry fermented sausage reformulated with camel meat and hump fat[J]. Journal of Food Measurement and Characterization,2017,11(2):758−767. doi: 10.1007/s11694-016-9445-6
    [16]
    CHEN J, NIU Y, WANG J, et al. Physicochemical property, bacterial diversity, and volatile profile during ripening of naturally fermented dry mutton sausage produced from Jianzhou big-eared goat[J]. Frontiers in Microbiology, 2022, 13: 961117.
    [17]
    牛茵, 尹礼国, 杨梓垚, 等. 不同加工环境自然发酵羊肉香肠细菌多样性与挥发性风味物质关联分析[J]. 现代食品科技,2023,39(1):270−280. [NIU Y, YIN L G, YANG Z Y, et al. Correlation analysis of bacterial communities and volatile flavor compounds of naturally fermented mutton sausages from different processing environments[J]. Modern Food Science and Technology,2023,39(1):270−280.]

    NIU Y, YIN L G, YANG Z Y, et al. Correlation analysis of bacterial communities and volatile flavor compounds of naturally fermented mutton sausages from different processing environments[J]. Modern Food Science and Technology, 2023, 391): 270280.
    [18]
    中华人民共和国国家卫生和计划生育委员会. GB 4789.41-2016 食品安全国家标准 食品微生物学检验 肠杆菌科检验[S]. 北京:中国标准出版社, 2016. [National Health and Family Planning Commission of the People's Republic of China. GB 4789.41-2016 National standard for food safety, Microbiology examination of food, Enterobacteriaceae examination[S] Beijing:China Standards Press, 2016.]

    National Health and Family Planning Commission of the People's Republic of China. GB 4789.41-2016 National standard for food safety, Microbiology examination of food, Enterobacteriaceae examination[S] Beijing: China Standards Press, 2016.
    [19]
    中华人民共和国国家卫生和计划生育委员会. GB 5009.237-2016 食品安全国家标准 食品pH值的测定[S]. 北京:中国标准出版社, 2016. [National Health and Family Planning Commission of the People's Republic of China. GB 5009.237-2016 National standard for food safety, Determination of pH value of food[S] Beijing:China Standards Press, 2016.]

    National Health and Family Planning Commission of the People's Republic of China. GB 5009.237-2016 National standard for food safety, Determination of pH value of food[S] Beijing: China Standards Press, 2016.
    [20]
    中华人民共和国国家卫生和计划生育委员会. GB 5009.33-2016 食品安全国家标准 食品中亚硝酸盐与硝酸盐的测定[S]. 北京:中国标准出版社, 2016. [National Health and Family Planning Commission of the People's Republic of China. GB 5009.33-2016 National standard for food safety, Determination of nitrite and nitrate in foodstuffs[S] Beijing:China Standards Press, 2016.]

    National Health and Family Planning Commission of the People's Republic of China. GB 5009.33-2016 National standard for food safety, Determination of nitrite and nitrate in foodstuffs[S] Beijing: China Standards Press, 2016.
    [21]
    中国国家标准化管理委员会. GB/T 35252-2017 动植物油脂 2-硫代巴比妥酸值的测定 直接法[S]. 北京:中国标准出版社, 2017. [Standardization Administration of China. GB/T 35252-2017 Animal and vegetable fats and oils— Determination of 2-thiobarbituric acid value—Direct method[S]. Beijing: Standards Press of China, 2017.]

    Standardization Administration of China. GB/T 35252-2017 Animal and vegetable fats and oils— Determination of 2-thiobarbituric acid value—Direct method[S]. Beijing: Standards Press of China, 2017.
    [22]
    刘登勇, 周光宏, 徐幸莲. 确定食品关键风味化合物的一种新方法:“ROAV”法[J]. 食品科学,2008(7):370−374. [LIU D Y, ZHOU G H, XU X L. “ROAV” Method:A new method for determining key odor compounds of rugao ham[J]. Food Science,2008(7):370−374.]

    LIU D Y, ZHOU G H, XU X L. “ROAV” Method: A new method for determining key odor compounds of rugao ham[J]. Food Science, 20087): 370374.
    [23]
    李珊珊. 传统发酵肉制品中发酵菌株的分离筛选及在牛肉发酵香肠中的应用初探[D]. 郑州:河南农业大学, 2021. [LI S S. Separation screening of fermented strain from traditional fermented meat products and application in beef fermented sausages[D]. Zhengzhou:Henan Agricultural University, 2021.]

    LI S S. Separation screening of fermented strain from traditional fermented meat products and application in beef fermented sausages[D]. Zhengzhou: Henan Agricultural University, 2021.
    [24]
    杨贝, 张香美, 卢涵, 等. 肉用发酵菌株的筛选及其在低盐发酵香肠中的应用[J]. 中国酿造, 2022(11):102-107. [YANG B, ZHANG X M, LU H, et al. Screening of strain for meat fermentation and its application in low-salt sausage fermentation[J] China Brewing, 2022(11):102-107.]

    YANG B, ZHANG X M, LU H, et al. Screening of strain for meat fermentation and its application in low-salt sausage fermentation[J] China Brewing, 2022(11): 102-107.
    [25]
    梁蕊芳, 张邦建, 高丽霞, 等. 复合发酵剂对发酵牛肉干理化品质及安全性能的影响[J]. 食品工业科技,2021,42(7):43−49. [LIANG R F, ZHANG B J, GAO L X, et al. Effects of starter cultures combination on physicochemical quality and safety of fermented beef jerky[J]. Science and Technology of Food Industry,2021,42(7):43−49.]

    LIANG R F, ZHANG B J, GAO L X, et al. Effects of starter cultures combination on physicochemical quality and safety of fermented beef jerky[J]. Science and Technology of Food Industry, 2021, 427): 4349.
    [26]
    CHEN Q, KONG B, HAN Q, et al. The role of bacterial fermentation in lipolysis and lipid oxidation in Harbin dry sausages and its flavour development[J]. LWT-Food Science and Technology,2017,77:389−396. doi: 10.1016/j.lwt.2016.11.075
    [27]
    郭玲玲. 接种植物乳杆菌和蔗糖添加量对发酵香肠品质特性的影响[J]. 肉类研究,2020,34(3):20−26. [GUO L L. Effects of inoculation with Lactobacillus plantarum and sucrose addition on quality characteristics of fermented sausage[J]. Meat Research,2020,34(3):20−26.]

    GUO L L. Effects of inoculation with Lactobacillus plantarum and sucrose addition on quality characteristics of fermented sausage[J]. Meat Research, 2020, 343): 2026.
    [28]
    中华人民共和国国家卫生和计划生育委员会. GB 2760-2014 食品安全国家标准 食品添加剂使用标准[S]. 北京:中国标准出版社, 2014. [National Health and Family Planning Commission of the People's Republic of China. GB 2760-2014 National standards for food safety, Standards for the use of food additives[S] Beijing:China Standards Press, 2014.]

    National Health and Family Planning Commission of the People's Republic of China. GB 2760-2014 National standards for food safety, Standards for the use of food additives[S] Beijing: China Standards Press, 2014.
    [29]
    牛雪峰. 发酵火腿中产香葡萄球菌的筛选及在发酵香肠中的应用[D]. 哈尔滨:哈尔滨商业大学, 2020. [NIU X F. Screening of Staphylococcus in fermented ham and its application in fermented sausage[D]. Harbin:Harbin University of Commerce, 2020.]

    NIU X F. Screening of Staphylococcus in fermented ham and its application in fermented sausage[D]. Harbin: Harbin University of Commerce, 2020.
    [30]
    夏秀芳, 张金铎, 孔保华. 木糖葡萄球菌添加量对发酵牛肉串品质特性的影响[J]. 食品科学,2013,34(7):47−50. [XIA X F, ZHANG J D, KONG B H. Effect of inoculum amount of Staphylococcus xylosus on quality characteristics of fermented beef kebabs[J]. Food Science,2013,34(7):47−50.]

    XIA X F, ZHANG J D, KONG B H. Effect of inoculum amount of Staphylococcus xylosus on quality characteristics of fermented beef kebabs[J]. Food Science, 2013, 347): 4750.
    [31]
    黄俊逸, 王凤娜, 吴香, 等. 复合发酵剂的筛选及其对发酵香肠加工过程中品质的影响[J]. 食品科学,2020,41(24):95−101. [HUANG J Y, WANG F N, WU X, et al. Effect of mixed starter cultures on the quality of fermented sausage[J]. Food Science,2020,41(24):95−101.] doi: 10.7506/spkx1002-6630-20191023-241

    HUANG J Y, WANG F N, WU X, et al. Effect of mixed starter cultures on the quality of fermented sausage[J]. Food Science, 2020, 4124): 95101. doi: 10.7506/spkx1002-6630-20191023-241
    [32]
    ZANARDI E, GHIDINI S, BATTAGLIA A, et al. Lipolysis and lipid oxidation in fermented sausages depending on different processing conditions and different antioxidants[J]. Meat Science,2004,66(2):415−423. doi: 10.1016/S0309-1740(03)00129-3
    [33]
    王德宝, 马文淑, 王柏辉, 等. 成熟时间对燕麦羊肉香肠食用品质、脂质氧化及风味物质积累的影响[J]. 食品与发酵工业,2020,46(11):191−198. [WANG D B, MA W S, WANG B H, et al. Effects of ripening time on edible quality, lipid oxidation and flavor accumulation of oat lamb sausage[J]. Food and Fermentation Industries,2020,46(11):191−198.]

    WANG D B, MA W S, WANG B H, et al. Effects of ripening time on edible quality, lipid oxidation and flavor accumulation of oat lamb sausage[J]. Food and Fermentation Industries, 2020, 4611): 191198.
    [34]
    杨滔, 张晓东, 刘红梅, 等. 不同发酵剂对发酵猪肉香肠品质的影响[J]. 食品工业科技,2022,43(1):101−109. [YANG T, ZHANG X D, LIU H M, et al. Effect of different starters on the quality of fermented pork sausage[J]. Science and Technology of Food Industry,2022,43(1):101−109.]

    YANG T, ZHANG X D, LIU H M, et al. Effect of different starters on the quality of fermented pork sausage[J]. Science and Technology of Food Industry, 2022, 431): 101109.
    [35]
    曹辰辰, 冯美琴, 孙健, 等. 功能性发酵剂对发酵香肠氧化稳定性及挥发性风味物质的影响[J]. 食品科学,2019,40(20):106−113. [CAO C C, FENG M Q, SUN J, et al. Effect of functional starter culture on antioxidant and volatile compound in fermented sausages[J]. Food Science,2019,40(20):106−113.]

    CAO C C, FENG M Q, SUN J, et al. Effect of functional starter culture on antioxidant and volatile compound in fermented sausages[J]. Food Science, 2019, 4020): 106113.
    [36]
    FRANKLIN L M, KING E S, CHAPMAN D, et al. Flavor and acceptance of roasted California almonds during accelerated storage[J]. Journal of Agricultural and Food Chemistry,2018,66(5):1222−1232. doi: 10.1021/acs.jafc.7b05295
    [37]
    刘英丽, 杨梓妍, 万真, 等. 发酵剂对发酵香肠挥发性风味物质形成的作用及影响机制研究进展[J]. 食品科学,2021,42(11):284−296. [LIU Y L, YANG Z Y, WAN Z, et al. Progress in understanding the effect and mechanism of starter cultures on the formation of volatile flavor compounds in fermented sausage[J]. Food Science,2021,42(11):284−296.] doi: 10.7506/spkx1002-6630-20201012-085

    LIU Y L, YANG Z Y, WAN Z, et al. Progress in understanding the effect and mechanism of starter cultures on the formation of volatile flavor compounds in fermented sausage[J]. Food Science, 2021, 4211): 284296. doi: 10.7506/spkx1002-6630-20201012-085
    [38]
    DOMÍNGUEZ R, PATEIRO M, GAGAOUA M, et al. A comprehensive review on lipid oxidation in meat and meat products[J]. Antioxidants,2019,8(10):429. doi: 10.3390/antiox8100429
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