Synergistic Effect of EGCG and Isorhamnetin on CellularAntioxidant Activity

PAN Junkun; JIAO Zhonggao; ZHANG Qiang

doi:10.13386/j.issn1002-0306.2023040160

Volume 44 Issue 22

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(22): 12-18. > DOI: 10.13386/j.issn1002-0306.2023040160

PAN Junkun, JIAO Zhonggao, ZHANG Qiang. Synergistic Effect of EGCG and Isorhamnetin on CellularAntioxidant Activity[J]. Science and Technology of Food Industry, 2023, 44(22): 12−18. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040160.

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Synergistic Effect of EGCG and Isorhamnetin on CellularAntioxidant Activity

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China

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Received Date: April 17, 2023
Available Online: September 16, 2023

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Abstract

Abstract

Objective: To investigate the synergistic antioxidant effects of different ratios of EGCG (epigallocatechin gallate) and isorhamnetin combinations, and provide a theoretical basis for the development of food borne flavonoid functional foods. Methods: The cellular antioxidant activities (CAA) of EGCG and isorhamnetiin combinations were estimated by Chou-Talalay combination Index (CI) method. Results: The median effective dose (EC₅₀) of EGCG and isorhamnetin combination (6:4, c/c) was 5.01±0.1 μg/mL with the CI_avg value of 0.76, which represented significantly synergistic effects. Further experiments proved that the superoxide dismutase (SOD) activities of EGCG+isorhamnetin (1.5+1, 3+2, and 6+4 μg/mL) were increased by 5.2%, 21.1% and 49.1%. Glutathione peroxidase (GSH-Px) activities were increased by 7.6%, 27.8% and 57.6%, and catalase (CAT) activities were increased by 5.6%, 24.6% and 42.1% of AAPH group value, respectively. Conclusion: The mechanism of the combination of EGCG and isorhamnetin in cell antioxidant activity might be through up-regulating the activity of endogenous antioxidant enzymes to enhance the cell's own antioxidant capacity, thereby achieving a balance in the body's oxidative stress response.
- EGCG,
- isorhamnetin,
- cellular antioxidant activity,
- synergistic effect,
- antioxidant enzymes

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