Analysis of Quality and Volatile Components in Different Varieties of Pumpkin

CHEN Linhong; FU Manqin; LU Shengyong; LI Lu; ZHONG Yujuan; LI Junxing; XIAO Gengsheng

doi:10.13386/j.issn1002-0306.2023040110

Volume 45 Issue 5

Feb. 2024

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Science and Technology of Food Industry > 2024 > 45(5): 223-233. > DOI: 10.13386/j.issn1002-0306.2023040110

CHEN Linhong, FU Manqin, LU Shengyong, et al. Analysis of Quality and Volatile Components in Different Varieties of Pumpkin[J]. Science and Technology of Food Industry, 2024, 45(5): 223−233. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040110.

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Analysis of Quality and Volatile Components in Different Varieties of Pumpkin

CHEN Linhong^{1, 2,},
FU Manqin^2, ,,
LU Shengyong^{2, 3},
LI Lu²,
ZHONG Yujuan⁴,
LI Junxing⁴,
XIAO Gengsheng^1, ,

1.
Food College of Light Industry, Zhongkai College of Agricultural Engineering, Guangzhou 510225, China
2.
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610, China
3.
Jiangmen Plant Protection Co., Ltd., Jiangmen 529000, China
4.
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China

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Received Date: April 15, 2023
Available Online: January 04, 2024

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Abstract

Abstract

Five varieties of pumpkins were used as the research objects to compare their physical and chemical properties, nutritional components, total phenols, antioxidant activity and volatile components. Grey correlation analysis was used to comprehensively evaluated the nine main quality characters of pumpkins, the volatile components of pumpkins were analyzed by headspace solid-phase microextraction and gas chromatography-mass spectrometry, and principal component analysis (PCA) was carried out. The results showed that the contents of dry matter, inositol and ascorbic acid in the five kind of pumpkins ranged from 36.95% to 50.91%, 1.48 to 17.02 mg/g and 2.17 to 15.26 mg/100 g, respectively, all of which were the highest value in Xiangyuanzao and the lowest value in 221-09. Xiangyuanzao had the highest pH value (7.95) and sucrose content (52.04 mg/g), the lowest value of total acid content (7.54 mg/kg), while Heixiaobao had the lowest pH value (7.26) and sucrose content (20.17 mg/g), and the highest value of total acid was 29.75 mg/kg. Starch content ranged from 1.06% to 3.65%, showing that 221-09 was the highest and Heixiaobao was the lowest. Fructose and glucose contents were ranged from 21.39 to 57.97 mg/g and 15.74 to 39.51 mg/g, respectively, both of which were the highest value in 221-09 and the lowest value in Xiangyuanzao. The total phenol content, DPPH value and ABTS value ranged from 2.44 to 3.00 mg GAE/g, 0.15 to 0.33 mg TE/g and 0.09 to 0.25 mg TE/g, respectively, the content of 221-09 was the highest and Xiangyuanyihao was the lowest. The results of grey correlation analysis showed that the Xiangyuanzao edge ranked first, with excellent overall quality, followed by 221-09. A total of 50 volatile aroma components were detected in 5 kinds of pumpkins. PCA analysis found that the Xiangyuanzao components were the most abundant and significantly different from other varieties. The flavor components with relative aroma activity value (ROAV) greater than 1 were 1-octene-3 alcohol, linalool, 2-methyl-2-butenal, hexanal, (E)-2-hexenal, nonanal, (E)-2-nonenal, (E,E)-3,5-octadien-2-one and beta-lonone, which were determined as the key aroma components. The results of this study can provide a reference for the excellent breeding, quality evaluation, processing and product development of pumpkin.
- pumpkin,
- quality,
- volatile components,
- grey correlation analysis

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References (44)

References

[1]	李新峥, 叶佳净, 陈学进, 等. 南瓜新品种百蜜嫩瓜1号的选育[J]. 中国瓜菜,2022,35(3):99−102. [LI X Z, YE J J, CHEN X J, et al. Selection and breeding of a new pumpkin variety Bainimengngua No. 1[J]. Chinese Gourd Vegetables,2022,35(3):99−102.] doi: 10.3969/j.issn.1673-2871.2022.03.018 LI X Z, YE J J, CHEN X J, et al. Selection and breeding of a new pumpkin variety Bainimengngua No. 1[J]. Chinese Gourd Vegetables, 2022, 35（3）: 99−102. doi: 10.3969/j.issn.1673-2871.2022.03.018
[2]	陈雅琪. 南瓜籽油Pickering乳液的制备及其稳定性与消化特性的研究[D]. 武汉:武汉轻工大学, 2022. [CHEN Y Q. Study on preparation, stability and digestive properties of pumpkin seed oil Pickering emulsion[D]. Wuhan:Wuhan Polytechnic University, 2022.] CHEN Y Q. Study on preparation, stability and digestive properties of pumpkin seed oil Pickering emulsion[D]. Wuhan: Wuhan Polytechnic University, 2022.
[3]	ANTONELA N G, SUZANA R B, MARIJA B S, et al. Carotenoid content and profiles of pumpkin products and by-products[J]. Molecules,2023,28(2):858. doi: 10.3390/molecules28020858
[4]	李俊星, 杨李益, 云天海. 南瓜加工品开发与利用研究进展[J]. 中国瓜菜,2018,31(4):1−4. [LI J X, YANG L Y, YUN T H. Research progress on the development and utilization of pumpkin processing products[J]. Chinese Gourd Vegetable,2018,31(4):1−4.] LI J X, YANG L Y, YUN T H. Research progress on the development and utilization of pumpkin processing products[J]. Chinese Gourd Vegetable, 2018, 31（4）: 1−4.
[5]	马玮, 史玉滋, 段颖, 等. 南瓜果实淀粉和可溶性固形物研究进展[J]. 中国瓜菜,2018,31(11):1−5. [MA W, SHI Y Z, DUAN Y, et al. Research progress on starch and soluble solids of pumpkin fruit[J]. Chinese Gourd Vegetables,2018,31(11):1−5.] MA W, SHI Y Z, DUAN Y, et al. Research progress on starch and soluble solids of pumpkin fruit[J]. Chinese Gourd Vegetables, 2018, 31（11）: 1−5.
[6]	HEMEI S, ZENGXIAN S. Hypolipidaemic and hypoglycaemic properties of pumpkin polysaccharides[J]. 3 Biotech,2017,7(3):159. doi: 10.1007/s13205-017-0843-1
[7]	谢元恒. 中国南瓜Bin图谱构建和果实相关性状QTL定位[D]. 广州:仲恺农业工程学院, 2020. [XIE Y H. Binmap constructing and QTL Mapping of fruit related traits in Cucurbita moschata pumpkin[D]. Guangzhou:Zhongkai University of Agriculture and Engineering, 2020.] XIE Y H. Binmap constructing and QTL Mapping of fruit related traits in Cucurbita moschata pumpkin[D]. Guangzhou: Zhongkai University of Agriculture and Engineering, 2020.
[8]	李健秀, 王树清, 景丽杰, 等. 植酸钠水解制取肌醇新工艺研究[J]. 化学世界,1998(11):583−585. [LI J X, WANG S Q, JING L J, et al. Study on the preparation of inositol by hydrolysis of sodium phytate[J]. Chem World,1998(11):583−585.] LI J X, WANG S Q, JING L J, et al. Study on the preparation of inositol by hydrolysis of sodium phytate[J]. Chem World, 1998（11）: 583−585.
[9]	卢引. 不同栽培品种南瓜对糖尿病的作用及挥发性成分的研究[D]. 郑州:河南大学, 2014. [LU Y. Study on the effects and volatile components of different cultivars of pumpkin on diabetes mellitus[D]. Zhengzhou:Henan University, 2014.] LU Y. Study on the effects and volatile components of different cultivars of pumpkin on diabetes mellitus[D]. Zhengzhou: Henan University, 2014.
[10]	李俊星, 钟玉娟, 罗剑宁, 等. 基于电子鼻与HS-SPME/GC-MS技术的香芋南瓜果实香气物质解析[J]. 现代食品科技,2018,34(9):244−250,297. [LI J X, ZHONG Y J, LUO J N, et al. Analysis of aroma substances of sweet potato and pumpkin fruit based on electronic nose and HS-SPME/GC-MS technology[J]. Modern Food Science and Technology,2018,34(9):244−250,297.] LI J X, ZHONG Y J, LUO J N, et al. Analysis of aroma substances of sweet potato and pumpkin fruit based on electronic nose and HS-SPME/GC-MS technology[J]. Modern Food Science and Technology, 2018, 34（9）: 244−250,297.
[11]	FU M Q, AN K J, XU Y J, et al. Effects of different temperature and humidity on bioactive flavonoids and antioxidant activity in Pericarpium Citri Reticulata ( Citrus reticulata ‘Chachi’)[J]. LWT,2018,93:167−173. doi: 10.1016/j.lwt.2018.03.036
[12]	YU Q, DUAN J, YU N, et al. Enhancing the antityrosinase activity of saponins and polyphenols from Asparagus by hot air coupled with microwave treatments[J]. LWT,2020,124:109174. doi: 10.1016/j.lwt.2020.109174
[13]	ARISE A K, ALASHI A M, NWACHUKWU I D, et al. Antioxidant activities of bambara groundnut ( Vigna subterranea) protein hydrolysates and their membrane ultrafiltration fractions[J]. Food & Function,2016,7(5):2431−2437.
[14]	FENG Y B, XU B G, YAGOUB E G A, et al. Role of drying techniques on physical, rehydration, flavor, bioactive compounds and antioxidant characteristics of garlic[J]. Food Chemistry,2020,343:128404.
[15]	任凯丽, 苏永全, 张化生, 等. 甘肃靖远旱砂西瓜挥发性香气成分分析[J]. 食品科学,2023,44(6):320−326. [REN K L, SU Y Q, ZHANG H S, et al. Analysis of volatile aroma components of watermelon from Jingyuan dry sand in Gansu Province[J]. Food Science,2023,44(6):320−326.] doi: 10.7506/spkx1002-6630-20220503-026 REN K L, SU Y Q, ZHANG H S, et al. Analysis of volatile aroma components of watermelon from Jingyuan dry sand in Gansu Province[J]. Food Science, 2023, 44（6）: 320−326. doi: 10.7506/spkx1002-6630-20220503-026
[16]	VERZERA A, DIMA G, TRIPODI G, et al. Fast quantitative determination of aroma volatile constituents in melon fruits by headspace-solid-phase microextraction and gas chromatography–mass spectrometry[J]. Food Analytical Methods,2011,4(2):141−149. doi: 10.1007/s12161-010-9159-z
[17]	LIANG Q, XIONG W, ZHOU Q, et al. Glucosinolates or erucic acid, which one contributes more to volatile flavor of fragrant rapeseed oil[J]. Food Chemistry,2023,412:135594. doi: 10.1016/j.foodchem.2023.135594
[18]	杨宏, 李跃建, 王长林, 等. 南瓜口感评价与营养成分的相关性和回归分析[J]. 中国蔬菜,2016(11):25−32. [YANG H, LI Y J, WANG C L, et al. Correlation and regression analysis of taste evaluation and nutritional components of pumpkin[J]. Chinese Vegetables,2016(11):25−32.] YANG H, LI Y J, WANG C L, et al. Correlation and regression analysis of taste evaluation and nutritional components of pumpkin[J]. Chinese Vegetables, 2016（11）: 25−32.
[19]	尹国香, 张焕春, 刘学卿, 等. 早熟南瓜新品种红阳南瓜的选育[J]. 中国蔬菜,2011(6):100−101. [YIN G X, ZHANG H C, LIU X J, et al. Breeding of a new early ripe pumpkin variety Hongyang gourd[J]. Chinese Vegetables,2011(6):100−101.] YIN G X, ZHANG H C, LIU X J, et al. Breeding of a new early ripe pumpkin variety Hongyang gourd[J]. Chinese Vegetables, 2011（6）: 100−101.
[20]	王锋, 黄文枫, 胡艳平, 等. 不同南瓜砧木对海南冬春季薄皮甜瓜生长发育及品质的影响[J]. 长江蔬菜,2022(20):50−53. [WANG F, HUANG W F, HU Y P, et al. Effects of different pumpkin rootstocks on growth and quality of thin-skin melon in Hainan in winter and spring[J]. Yangtze River Vegetables,2022(20):50−53.] WANG F, HUANG W F, HU Y P, et al. Effects of different pumpkin rootstocks on growth and quality of thin-skin melon in Hainan in winter and spring[J]. Yangtze River Vegetables, 2022（20）: 50−53.
[21]	关博洋, 殷菲胧, 刘云芬, 等. 贮藏温度对采后龙眼果实糖代谢及其相关酶活性的影响[J]. 食品工业科技,2022,43(5):348−355. [GUAN B Y, YIN F L, LIU Y F, et al. Effect of storage temperature on glucose metabolism and related enzyme activities of postharvety longan fruit[J]. Science and Technology of Food Industry,2022,43(5):348−355.] GUAN B Y, YIN F L, LIU Y F, et al. Effect of storage temperature on glucose metabolism and related enzyme activities of postharvety longan fruit[J]. Science and Technology of Food Industry, 2022, 43（5）: 348−355.
[22]	SONNEWALD U, FERNIE A R. Next-generation strategies for understanding and influencing source-sink relations in crop plants[J]. Current Opinion in Plant Biology,2018,43:63−70. doi: 10.1016/j.pbi.2018.01.004
[23]	DARRUDI R, NAZERI V, SOLTANI F, et al. Evaluation of combining ability in Cucurbita pepo L. and Cucurbita moschata Duchesne accessions for fruit and seed quantitative traits[J]. Journal of Applied Research on Medicinal and Aromatic Plants,2018,9:70−77. doi: 10.1016/j.jarmap.2018.02.006
[24]	CORRIGAN V K, IRVING D E, POTTER J F. Sugars and sweetness in buttercup squash[J]. Food Quality and Preference,2000,11(4):313−322. doi: 10.1016/S0950-3293(99)00077-4
[25]	王安君. 基于RNA-Seq的南瓜果实重要品质形成的代谢和分子调控研究[D]. 广州:暨南大学, 2017. [WANG A J. Metabolic and molecular regulation of important quality formation in pumpkin fruit based on RNA-Seq[D]. Guangzhou:Jinan University, 2017.] WANG A J. Metabolic and molecular regulation of important quality formation in pumpkin fruit based on RNA-Seq[D]. Guangzhou: Jinan University, 2017.
[26]	杨晓惠, 韦云路, 李菲, 等. 南瓜活性成分及其深加工食品研究进展[J]. 粮食与油脂,2021,34(7):4−7. [YANG X H, WEI Y L, LI F, et al. Research progress on active ingredients of pumpkin and its deep processed food[J]. Grain & Oils,2021,34(7):4−7.] doi: 10.3969/j.issn.1008-9578.2021.07.002 YANG X H, WEI Y L, LI F, et al. Research progress on active ingredients of pumpkin and its deep processed food[J]. Grain & Oils, 2021, 34（7）: 4−7. doi: 10.3969/j.issn.1008-9578.2021.07.002
[27]	XIA T, WANG Q. Hypoglycaemic role of Cucurbita ficifolia (Cucurbitaceae) fruit extract in streptozotocin-induced diabetic rats[J]. Journal of the Science of Food and Agriculture,2007,87(9):1753−1757. doi: 10.1002/jsfa.2916
[28]	熊玲, 陈京晓, 牟明远, 等. 南瓜的营养保健价值分析及产品的开发现状[J]. 食品工业科技,2013,34(23):395−400. [XIONG L, CHEN J X, MOU M Y, et al. Nutritional and health value analysis and product development status of pumpkin[J]. Science and Technology of Food Industry,2013,34(23):395−400.] XIONG L, CHEN J X, MOU M Y, et al. Nutritional and health value analysis and product development status of pumpkin[J]. Science and Technology of Food Industry, 2013, 34（23）: 395−400.
[29]	杨红娟, 顾卫红, 唐庆久, 等. 富肌醇南瓜种质资源的创新与利用[J]. 植物遗传资源学报,2012,13(1):157−162. [YANG H J, GU W H, TANG Q J, et al. Innovation and utilization of myo-inositol-rich pumpkin germplasm[J]. Journal of Plant Genetic Resources,2012,13(1):157−162.] YANG H J, GU W H, TANG Q J, et al. Innovation and utilization of myo-inositol-rich pumpkin germplasm[J]. Journal of Plant Genetic Resources, 2012, 13（1）: 157−162.
[30]	DUYEN H. H N, HASSAN E, XHENSILA L, et al. Chemical composition and health attributes of agri-foods:A Scientific overview on black foods[J]. Sustainability,2023,15(4):3852−3852. doi: 10.3390/su15043852
[31]	锁冠文. 超声波处理对南瓜汁贮藏期间品质的影响[D]. 南昌:江西科技师范大学, 2022. [SUO G W. Effect of ultrasonic treatment on the quality of pumpkin juice during storage[D]. Nanchang:Jiangxi Science and Technology Normal University, 2022.] SUO G W. Effect of ultrasonic treatment on the quality of pumpkin juice during storage[D]. Nanchang: Jiangxi Science and Technology Normal University, 2022.
[32]	WANG H, CAO G H, PRIOR, R L. Total antioxidant capacity of fruits[J]. Journal of Agricultural and Food Chemistry,1996,44(3):701−705. doi: 10.1021/jf950579y
[33]	LEICHTWEIS M G, MOLINA A K, PIRES T C. S, et al. Biological activity of pumpkin byproducts:Antimicrobial and antioxidant properties[J]. Molecules,2022,27(23):8366−8381. doi: 10.3390/molecules27238366
[34]	李晓颍, 宋立琴, 李明媛, 等. 果实挥发性成分的生物合成与代谢调控研究进展[J]. 农业生物技术学报,2023,31(3):629−642. [LI X Y, SONG L Q, LI M Y, et al. Research progress on biosynthesis and metabolic regulation of volatile components in fruits[J]. Journal of Agricultural Biotechnology,2023,31(3):629−642.] LI X Y, SONG L Q, LI M Y, et al. Research progress on biosynthesis and metabolic regulation of volatile components in fruits[J]. Journal of Agricultural Biotechnology, 2023, 31（3）: 629−642.
[35]	陈敏氡, 王彬, 刘建汀, 等. 南瓜5个品种果肉的挥发性成分分析[J]. 热带亚热带植物学报,2020,28(6):633−643. [CHEN M D, WANG B, LIU J T, et al. Analysis of volatile components in pulp of five pumpkin varieties[J]. Chin J Botanica Sinica,2020,28(6):633−643.] CHEN M D, WANG B, LIU J T, et al. Analysis of volatile components in pulp of five pumpkin varieties[J]. Chin J Botanica Sinica, 2020, 28（6）: 633−643.
[36]	吕世懂, 吴远双, 姜玉芳, 等. 不同产区乌龙茶香气特征及差异分析[J]. 食品科学,2014,35(2):146−153. [LÜ S D, WU Y S, JIANG Y F, et al. Aroma characteristics and difference analysis of Oolong tea from different producing regions[J]. Food Science,2014,35(2):146−153.] LÜ S D, WU Y S, JIANG Y F, et al. Aroma characteristics and difference analysis of Oolong tea from different producing regions[J]. Food Science, 2014, 35（2）: 146−153.
[37]	刘潇. 高温胁迫下电子束辐照对储藏籼稻脂质变化规律的影响机制研究[D]. 南京:南京财经大学, 2023. [LIU X. Effect mechanism of electron beam irradiation on lipid changes in indica rice under high temperature stress[D]. Nanjing:Nanjing University of Finance & Economics, 2023.] LIU X. Effect mechanism of electron beam irradiation on lipid changes in indica rice under high temperature stress[D]. Nanjing: Nanjing University of Finance & Economics, 2023.
[38]	陈琪. 辣椒红油的制备、光稳定性研究及其在风干肠的应用[D]. 成都:成都大学, 2023. [CHEN Q. Study on preparation and photostability of capsicum red oil and its application in air-dried intestine[D]. Chengdu:Chengdu University, 2023.] CHEN Q. Study on preparation and photostability of capsicum red oil and its application in air-dried intestine[D]. Chengdu: Chengdu University, 2023.
[39]	周小男. 薏米面条加工和贮藏过程中脂肪的酸败及风味变化研究[D]. 哈尔滨:东北农业大学, 2022. [ZHOU X N. Study on fat rancidity and flavor changes during processing and storage of job's tears noodles[D]. Harbin:Northeast Agricultural University, 2022.] ZHOU X N. Study on fat rancidity and flavor changes during processing and storage of job's tears noodles[D]. Harbin: Northeast Agricultural University, 2022.
[40]	陈磊, 毕秀芳, 焦文成, 等. 不同品牌泡姜中关键挥发性风味物质分析[J]. 中国调味品,2022,47(11):151−157,172. [CHEN L, BI X F, JIAO W C, et al. Analysis of key volatile flavor compounds in different brands of pickled ginger[J]. Chinese Condiments,2022,47(11):151−157,172.] CHEN L, BI X F, JIAO W C, et al. Analysis of key volatile flavor compounds in different brands of pickled ginger[J]. Chinese Condiments, 2022, 47（11）: 151−157,172.
[41]	张馨文. 超高压处理对沃柑汁品质的影响[D]. 南宁:广西大学, 2022. [ZHANG X W. Effect of ultra-high pressure treatment on the quality of citrus juice[D]. Nanning:Guangxi University, 2022.] ZHANG X W. Effect of ultra-high pressure treatment on the quality of citrus juice[D]. Nanning: Guangxi University, 2022.
[42]	LIU N F, SHEN S S, HUANG L F, et al. Revelation of volatile contributions in green teas with different aroma types by GC-MS and GC-IMS[J]. Food Research International,2023,169:112845. doi: 10.1016/j.foodres.2023.112845
[43]	谢永恒. 甜瓜中关键香气物质鉴定及其相互作用研究[D]. 上海:上海应用技术大学, 2021. [XIE Y H. Identification and interaction of key aroma substances in melon[D]. Shanghai:Shanghai Institute of Technology, 2021.] XIE Y H. Identification and interaction of key aroma substances in melon[D]. Shanghai: Shanghai Institute of Technology, 2021.
[44]	王佳童. 茶枝柑-白茶关键品质化学成分研究[D]. 武汉:华中农业大学, 2022. [WANG J T. Study on chemical constituents of key quality of Mandarin and white tea[D]. Wuhan:Huazhong Agricultural University, 2022.] WANG J T. Study on chemical constituents of key quality of Mandarin and white tea[D]. Wuhan: Huazhong Agricultural University, 2022.

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