Optimization of Microwave-assisted Deep Eutectic Solvent Extraction of Total Flavonoids from <i>Paeonia rockii</i> Seeds and Its Antioxidant Activity

CHEN Siyu; RAO Guiwei; WANG Luhua; SHENG Yingfei

doi:10.13386/j.issn1002-0306.2023030318

Volume 45 Issue 5

Feb. 2024

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Science and Technology of Food Industry > 2024 > 45(5): 161-168. > DOI: 10.13386/j.issn1002-0306.2023030318

CHEN Siyu, RAO Guiwei, WANG Luhua, et al. Optimization of Microwave-assisted Deep Eutectic Solvent Extraction of Total Flavonoids from Paeonia rockii Seeds and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2024, 45(5): 161−168. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023030318.

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Optimization of Microwave-assisted Deep Eutectic Solvent Extraction of Total Flavonoids from Paeonia rockii Seeds and Its Antioxidant Activity

1.
Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China
2.
Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, China
3.
College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China

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Received Date: March 28, 2023
Available Online: January 03, 2024

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Abstract

Abstract

Objective: To optimize the extraction method of total flavonoids from Peony purpurea seeds with microwave-assisted deep eutectic solvent, and to investigate its antioxidant activity in vitro. Methods: Microwave-assisted technology using deep eutectic solvent as extraction solvent was used to extract total flavonoids from Paeonia rockii seeds. Single factorial experiments and response surface methodology optimized the extraction process. The in vitro antioxidant activity of the total flavonoids was evaluated in terms of the rate of scavenging of DPPH radicals and the rate of scavenging of hydroxyl radicals. Results: Under the deep eutectic solvent system with chloride as hydrogen bond acceptor and urea as hydrogen bond donor, the deep eutectic solvent system with a molar ratio of 1:3 and a water content of 41.80% (V/V) was optimal. The ratio of material to liquid was 1:19 g/mL, the power of the microwave was 110 W and the time of the microwave was 3.00 min. The total flavonoid yield from Paeonia rockii seeds was 1.76 mg/g. When the total flavonoid concentration of the extract was 80 μg/mL, the scavenging rate of DPPH reached 82.00%. And the scavenging rate of hydroxyl radicals was 42.10% when the total flavonoid concentration of the extract was up to 80 μg/mL. Conclusion: Microwave-assisted deep eutectic solvent extraction of total flavonoids from Paeonia rockii seeds has high content, antioxidant activity and DPPH free radical scavenging rate of more than 80%.
- Paeonia rockii seeds,
- flavonoids,
- deep eutectic solvent,
- microwave-assisted extraction,
- antioxidant

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[1]	HAN L Y, ZHAI Y H, WANG Y M, et al. Diacylglycerol acyltransferase 3(DGAT3) is responsible for the biosynthesis of unsaturated fatty acids in vegetative organs of Paeonia rockii[J]. International Journal of Molecular Sciences,2022,23(22):14390. doi: 10.3390/ijms232214390
[2]	王军. 三种油用牡丹品质测定对比研究[J]. 中国农业文摘-农业工程,2021,33(3):57−60. [WANG J. Comparison study on the quality measurement of three oil peonies[J]. Agricultural Science and Engineering, in China,2021,33(3):57−60.] WANG J. Comparison study on the quality measurement of three oil peonies[J]. Agricultural Science and Engineering, in China, 2021, 33（3）: 57−60.
[3]	张姗姗, 赵凡, 魏小豹, 等. ‘凤丹’和紫斑牡丹6个产地种子脂肪酸组分的比较[J]. 中国粮油学报,2021,36(3):84−90. [ZHANG S S, ZHAO F, WEI X B, et al. Comparison of seed oil fatty acids between Paeonia ostii 'Fengdan' and P. rockii from 6 regions[J]. Journal of the Chinese Cereals and Oils,2021,36(3):84−90.] ZHANG S S, ZHAO F, WEI X B, et al. Comparison of seed oil fatty acids between Paeonia ostii 'Fengdan' and P. rockii from 6 regions[J]. Journal of the Chinese Cereals and Oils, 2021, 36（3）: 84−90.
[4]	刘普, 牛亚琪, 邓瑞雪, 等. 紫斑牡丹籽饼粨低聚芪类成分研究[J]. 中国药学杂志,2014,49(12):1018−1021. [LIU P, NIU Y Q, DENG R X, et al. Oligostibenes study from seed cakes of Paeonia rockii[J]. Chiese Pharmaceutical Journal,2014,49(12):1018−1021.] LIU P, NIU Y Q, DENG R X, et al. Oligostibenes study from seed cakes of Paeonia rockii[J]. Chiese Pharmaceutical Journal, 2014, 49（12）: 1018−1021.
[5]	YU S, DU S, YUAN J, et al. Fatty acid profile in the seeds and seed tissues of Paeonia L. species as new oil plant resources[J]. Scientific Reports,2016,6(1):1−10. doi: 10.1038/s41598-016-0001-8
[6]	饶桂维, 梅瑜, 陈贝贝, 等. 快速溶剂萃取法提取枇杷核中总黄酮的研究[J]. 食品工业科技,2013,34(19):213−215. [RAO G W, MEI Y, CHEN B B, et al. Extraction of total flavones from loquat kernel by accelerated solvent extraction[J]. Science and Technology of Food Industry,2013,34(19):213−215.] RAO G W, MEI Y, CHEN B B, et al. Extraction of total flavones from loquat kernel by accelerated solvent extraction[J]. Science and Technology of Food Industry, 2013, 34（19）: 213−215.
[7]	孔方, 李莉, 刘言娟. 超声辅助低共熔溶剂提取苹果叶中的总黄酮[J]. 食品工业科技,2020,41(14):134−139,147. [KONG F, LI L, LIU Y J. Ultrasonic-assisted deep eutectic solvents extraction of total flavonoids from apple leaves[J]. Science and Technology of Food Industry,2020,41(14):134−139,147.] KONG F, LI L, LIU Y J. Ultrasonic-assisted deep eutectic solvents extraction of total flavonoids from apple leaves[J]. Science and Technology of Food Industry, 2020, 41（14）: 134−139,147.
[8]	张雪, 刘娅, 刘秀敏, 等. 超声辅助低共熔溶剂提取新疆红枣中环磷酸腺苷的工艺优化[J]. 食品工业科技, 2022, 43(14):243−250. [ZHANG X, LIU Y, LIU X M, et al. Optimization of ultrasound-assisted extraction of cyclic adenosine 3’,5´-monophosphate from Xinjiang jujube by deep eutecitic solvent[J]. Science and Technology of Food Industry, 2021, 43(14):243−250.] ZHANG X, LIU Y, LIU X M, et al. Optimization of ultrasound-assisted extraction of cyclic adenosine 3’,5´-monophosphate from Xinjiang jujube by deep eutecitic solvent[J]. Science and Technology of Food Industry, 2021, 43（14）: 243−250.
[9]	刘超, 黎维维, 雷杰, 等. 低共熔溶剂提取江南卷柏穗花杉双黄酮的工艺优化[J]. 食品工业科技,2020,43(16):176−184. [LIU C, LI W W, LEI J, et al. Deep eutectic solvents extraction and optimization of amentoflacone from Selaginella moellendorffii[J]. Science and Technology of Food Industry,2020,43(16):176−184.] LIU C, LI W W, LEI J, et al. Deep eutectic solvents extraction and optimization of amentoflacone from Selaginella moellendorffii[J]. Science and Technology of Food Industry, 2020, 43（16）: 176−184.
[10]	SONG Z, LI X X, CHAO H, et al. Computer-aided ionic liquid design for alkane/cycloalkane extractive distillation process[J]. Green Energy & Environment,2019,4(2):154−165.
[11]	DUQUE A, GRAU J, BENEDE J L, et al. Low toxicity deep eutectic solvent-based ferrofluid for the determination of UV filters in environmental waters by stir bar dispersive liquid microextraction[J]. Talanta,2022,243:123378. doi: 10.1016/j.talanta.2022.123378
[12]	ZHANG H L, HAO F L, YAO Z F, et al. Efficient extraction of flavonoids from Polygonatum sibiricum using a deep eutectic solvent as a green extraction solvent[J]. Microchemical Journal,2022,175:107168. doi: 10.1016/j.microc.2021.107168
[13]	李吉超. 低共熔溶剂提取银杏叶中黄酮及纯化研究[D]. 北京:北京化工大学, 2020. [LI J C. Extraction and purification of flavonoids from Ginkgo biloba leaves with low eutectic solvent[D]. Beijing:Beijing University of Chemical Technology, 2020.] LI J C. Extraction and purification of flavonoids from Ginkgo biloba leaves with low eutectic solvent[D]. Beijing: Beijing University of Chemical Technology, 2020.
[14]	栾琳琳, 卢红梅, 陈莉, 等. 微波辅助低共熔溶剂提取桑葚果渣花青素的工艺研究[J]. 中国调味品,2020,45(5):191−196. [LUAN L L, IU H M, CHEN L, et al. Research on process of microwave-assisted low-eutectic solvent extraction of anthocyanins from mulberry residues[J]. Chinese Condiment,2020,45(5):191−196.] LUAN L L, IU H M, CHEN L, et al. Research on process of microwave-assisted low-eutectic solvent extraction of anthocyanins from mulberry residues[J]. Chinese Condiment, 2020, 45（5）: 191−196.
[15]	孙悦, 刘晓冰, 苏卓文, 等. 辅助低共熔溶剂提取鹰嘴豆中黄酮及其抗氧化活性的研究[J]. 食品工业科技,2020,41(14):120−128. [SUN Y, LIU X B, SU Z W, et al. Extraction of flavonoids from chickpeas by microwave-assisted eutectic solvent and its antioxidant activity[J]. Science and Technology of Food Industry,2020,41(14):120−128.] SUN Y, LIU X B, SU Z W, et al. Extraction of flavonoids from chickpeas by microwave-assisted eutectic solvent and its antioxidant activity[J]. Science and Technology of Food Industry, 2020, 41（14）: 120−128.
[16]	石欣隆, 刘伟, 张琳, 等. 紫斑牡丹籽产量及籽油品质综合评价[J]. 河南科技大学学报(自然科学版),2020,41(6):73−80. [SHI X L, LIU W, ZHANG L, et al. Comprehensive evaluation of seed yield and seed oil quality of Paeonia rockii[J]. Journal of Henan University of Science and Technology (Natural Science),2020,41(6):73−80.] SHI X L, LIU W, ZHANG L, et al. Comprehensive evaluation of seed yield and seed oil quality of Paeonia rockii[J]. Journal of Henan University of Science and Technology （Natural Science）, 2020, 41（6）: 73−80.
[17]	高康俊, 饶桂维. 响应面法优化超声辅助提取紫斑牡丹籽白藜芦醇工艺[J]. 浙江树人大学学报,2022,22(2):31−39. [GAO K J, RAO G W. Optimization of ultrasonic assisted extraction of resveratrol from Paeonia purpurea seeds by response surface method[J]. Journal of Zhejiang Shuren University,2022,22(2):31−39.] GAO K J, RAO G W. Optimization of ultrasonic assisted extraction of resveratrol from Paeonia purpurea seeds by response surface method[J]. Journal of Zhejiang Shuren University, 2022, 22（2）: 31−39.
[18]	刘洋旋, 吕庆云, 党允卓. 响应面法优化超声辅助低共熔溶剂提取葛根中黄酮及其抗氧化分析[J]. 食品科技, 2021, 46(11):195−202. [LIU Y X, LÜ Q Y, DANG Y Z. Optimization of extraction of flavonoids from Pueraria root with ultrasonic-assisted eutectic solvent and its antioxidant analysis by response surface method[J]. Journal of Food Science and Technology, 2021, 46(11):195−202.] LIU Y X, LÜ Q Y, DANG Y Z. Optimization of extraction of flavonoids from Pueraria root with ultrasonic-assisted eutectic solvent and its antioxidant analysis by response surface method[J]. Journal of Food Science and Technology, 2021, 46（11）: 195−202.
[19]	QI C, JU Z L, LI T. Sustainable deep eutectic solvents preparation and their efficiency in extraction and enrichment of main bioactive flavonoids from sea buckthorn leaves[J]. Journal of Cleaner Production,2018,184(5):826−835.
[20]	李梅, 卢秋榕, 韦迎春. 微波辅助低共熔溶剂提取薏苡叶总黄酮的工艺研究[J]. 中国食品添加剂,2017(5):49−53. [LI M, LU Q R, WEI Y C. Study on microwave-assisted extraction of total flavonoids from Coix lacrima- Coix Leaves with low eutectic solvent[J]. China Food Additives,2017(5):49−53.] LI M, LU Q R, WEI Y C. Study on microwave-assisted extraction of total flavonoids from Coix lacrima-Coix Leaves with low eutectic solvent[J]. China Food Additives, 2017（5）: 49−53.
[21]	童天娇. 深共熔溶剂提取黄酮类化合物的方法研究[D]. 西安:陕西理工大学, 2022:13-21. [TONG T J. Study on the extraction method of flavonoid by deep eutectic solvent[D]. Xi’an: Shaanxi University of Technology, 2022:13-21.] TONG T J. Study on the extraction method of flavonoid by deep eutectic solvent[D]. Xi’an: Shaanxi University of Technology, 2022: 13-21.
[22]	王丽丽, 林清霞, 宋振硕, 等. 分光光度法测定茶叶中总黄酮含量[J]. 茶叶学报,2021,62(1):1−6. [WANG L L, LIN Q X, SONG Z S. et al. Spectrophotometric determination of total flavonouids in tea[J]. Acta Tea Sinica,2021,62(1):1−6.] WANG L L, LIN Q X, SONG Z S. et al. Spectrophotometric determination of total flavonouids in tea[J]. Acta Tea Sinica, 2021, 62（1）: 1−6.
[23]	王斌利, 张莉霞, 石晓峰. 响应面法优化紫斑牡丹花粉总黄酮的提取工艺[J]. 中药材,2017,40(10):2396−2400. [WANG B L, ZHANG L X, SHI X F. Optimization of extraction technology of total flavonoids from pollen of peony purple by response surface method[J]. Chinese Traditional Medicine,2017,40(10):2396−2400.] WANG B L, ZHANG L X, SHI X F. Optimization of extraction technology of total flavonoids from pollen of peony purple by response surface method[J]. Chinese Traditional Medicine, 2017, 40（10）: 2396−2400.
[24]	阮尚全, 张红, 何正. 微波提取茶树叶中黄酮及清除DPPH·活性研究[J]. 内江师范学院学报,2019,34(12):57−61. [RUAN S Q, ZHANG H, HE Z. On extracton of flavonoids in tea leaves by microwave and scavenging of DPPH activity[J]. Journal of Neijiang Normal University,2019,34(12):57−61.] RUAN S Q, ZHANG H, HE Z. On extracton of flavonoids in tea leaves by microwave and scavenging of DPPH activity[J]. Journal of Neijiang Normal University, 2019, 34（12）: 57−61.
[25]	孙晓波, 张晓纯, 郭松, 等. 响应面法优化蒲桃叶总黄酮的提取工艺及其抗氧化活性[J]. 食品研究与开发,2022,43(2):115−122. [SUN X B, ZHANG X C, GUO S. Optimization of total flavonoids extraction from Syzygium jambos leaves by response surface methodology and its antioxidant activity[J]. Food Research and Development,2022,43(2):115−122.] SUN X B, ZHANG X C, GUO S. Optimization of total flavonoids extraction from Syzygium jambos leaves by response surface methodology and its antioxidant activity[J]. Food Research and Development, 2022, 43（2）: 115−122.
[26]	钱镭, 任德财, 姜涛. 响应面法优化紫薯中花色苷的提取工艺[J]. 中国调味品,2018,43(10):170−175. [QIAN L, REN D C, JIANG T. Optimization of extraction process of anthocyanins from purple potato by response surface method[J]. Chinese Condiments,2018,43(10):170−175.] QIAN L, REN D C, JIANG T. Optimization of extraction process of anthocyanins from purple potato by response surface method[J]. Chinese Condiments, 2018, 43（10）: 170−175.
[27]	李洁, 石晓峰, 叶倩女. Plackett-Burman设计结合Box-Behnken响应面法优化紫斑牡丹籽壳中低聚芪类和单萜苷类化合物的超声提取工艺[J]. 中国现代应用药学,2023,40(1):68−74. [LI J, SHI X F, YE Q N. Optimization of the ultrasonic extraction process of the seed coat from Paeoniarockii (S. G. Haw. et. Laeuner) T. Hanget. T. J. Li by Plackett-Burman design combined with box-behnken response surface method[J]. Chinese Journal of Modern Applied Pharmacy,2023,40(1):68−74.] LI J, SHI X F, YE Q N. Optimization of the ultrasonic extraction process of the seed coat from Paeoniarockii （S. G. Haw. et. Laeuner） T. Hanget. T. J. Li by Plackett-Burman design combined with box-behnken response surface method[J]. Chinese Journal of Modern Applied Pharmacy, 2023, 40（1）: 68−74.
[28]	李颖, 侯诗, 廖婉婷, 等. 响应面法优化黄晶果果皮总黄酮提取工艺及抗氧化活性[J]. 食品科技,2022,47(7):175−181. [LI Y, HOU S, LIAO W T, et al. Optimization of extraction process by response surface method and antioxidant activity of total flavonoids from Abiu. peels[J]. Food Science and Technology,2022,47(7):175−181.] LI Y, HOU S, LIAO W T, et al. Optimization of extraction process by response surface method and antioxidant activity of total flavonoids from Abiu. peels[J]. Food Science and Technology, 2022, 47（7）: 175−181.
[29]	BALIYAN S, MUKHERJEE R, PRIYADARSHINI A, et al. Determination of antioxidants by DPPH Radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa[J]. Molecules,2022,27(4):1326. doi: 10.3390/molecules27041326
[30]	杨怡萌, 杨广梅, 王仲明, 等. 荷叶黄酮自由基清除活性的量子化学研究[J]. 分子科学学报,2020,36(6):489−496. [YANG Y M, YANG G M, WANG Z M, et al. Quantum chemical study on radical scavenging activity of lotus leaf flavonoids[J]. Journal of Molecular Sciences,2020,36(6):489−496.] YANG Y M, YANG G M, WANG Z M, et al. Quantum chemical study on radical scavenging activity of lotus leaf flavonoids[J]. Journal of Molecular Sciences, 2020, 36（6）: 489−496.
[31]	谢建华, 陈思杨, 张桂云, 等. 柚皮总黄酮的提取优化及其对亚硝酸盐清除作用的影响[J]. 长春师范大学学报,2020,39(8):82−88. [XIE J H, CHEN S Y, ZHANG G Y, et al. Extraction optimization of total flavonoids from pomelo peel and its effect on nitrite removal[J]. Journal of Changchun Normal University,2020,39(8):82−88.] XIE J H, CHEN S Y, ZHANG G Y, et al. Extraction optimization of total flavonoids from pomelo peel and its effect on nitrite removal[J]. Journal of Changchun Normal University, 2020, 39（8）: 82−88.

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