Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis

LIU Rongxu; LI Chunyu; WANG Yucong; XIE Zhixin; XIE Yitong; LI Shuangpeng; LIU Danyi; HAN Jianchun

doi:10.13386/j.issn1002-0306.2023010016

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 99-107. > DOI: 10.13386/j.issn1002-0306.2023010016

LIU Rongxu, LI Chunyu, WANG Yucong, et al. Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis[J]. Science and Technology of Food Industry, 2023, 44(19): 99−107. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023010016.

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Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis

1.
Heilongjiang Green Food Science Research Institute, Harbin 150028, China
2.
College of Food Science, Northeast Agricultural University, Harbin 150030, China

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Received Date: January 03, 2023
Available Online: August 04, 2023

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Abstract

Abstract

Hemp protein isolate (HPI) was used as raw material to modify HPI through ultra-high pressure assisted enzymatic hydrolysis reaction. The solubility and degree of hydrolysis were used as criteria to screen the optimal conditions for the enzymatic hydrolysis modification. The influence of ultra-high pressure assisted enzymatic hydrolysis on solubility, foaming, emulsification, water retention and oil retention of enzymatic hydrolysis products was explored. The results showed that the optimum conditions for HPI enzymatic reaction were as follows: Adding amount of enzyme (complex protease) 5000 U/g, pH8.0, temperature 55 ℃ and time 50 min. Taking HPI as the control, when the pressure was 200 MPa, the solubility, foaming property, emulsification property and oil holding capacity of enzymatic hydrolysis products were the highest, when the pressure was 100 MPa, the foaming stability was the best, the emulsification stability after enzymatic hydrolysis had different degrees of decline, when the pressure was 0.1 MPa, the water holding capacity reached the maximum. In conclusion, the ultrahigh pressure technology can effectively promote the enzymatic hydrolysis modification reaction of HPI, and the physical and chemical properties of the enzymatic hydrolysis products were the best when the pressure is 200 MPa.
- hemp protein isolate (HPI),
- ultra-high pressure processing (UHP),
- enzymatic hydrolysis,
- physicochemical property

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[1]	王冰, 杨杨, 范洪臣, 等. 热处理温度和离子强度对汉麻蛋白热聚集行为的影响[J]. 食品安全质量检测学报,2022,13(16):5337−5344. [WANG Bing, YANG Yang, FAN Hongchen, et al. Effects of heat treatment temperature and ionic strength on thermal aggregation behavior of hemp protein[J]. Journal of Food Safety & Quality,2022,13(16):5337−5344. WANG Bing, YANG Yang, FAN Hongchen, al. Effects of heat treatment temperature and ionic strength on thermal aggregation behavior of hemp protein[J]. Journal of Food Safety & Quality, 2022, 13(16): 5337-5344.
[2]	石杰, 宋淑敏, 魏连会, 等. 汉麻籽蛋白的分级提取及产物功能特性的研究[J]. 粮食与油脂,2021,34(11):132−136. [SHI Jie, SONG Shumin, WEI Lianhui, et al. Study on fractionation of hemp seed protein and functional properties of the product[J]. Cereals & Oils,2021,34(11):132−136. SHI Jie, SONG Shumin, WEI Lianhui, et al. Study on fractionation of hemp seed protein and functional properties of the product[J]. Cereals & Oils, 2021, 34(11): 132-136.
[3]	MALOMO S A, RONG H, ALUKO R E. Structural and functional properties of hemp seed protein products[J]. Journal of Food Science,2014,79(8):1512−1521. doi: 10.1111/1750-3841.12537
[4]	庞佳坤, 郑远荣, 刘振民, 等. 超高压对乳清分离蛋白结构和抗氧化活性的影响[J]. 食品与发酵工业,2020,46(4):72−77. [PANG Jiakun, ZHENG Yuanrong, LIU Zhenmin, et al. Effects of ultra-high pressure on structure and antioxidant activity of whey protein isolates[J]. Food and Fermentation Industries,2020,46(4):72−77. PANG Jiakun, ZHENG Yuanrong, LIU Zhenmin, et al. Effects of ultra-high pressure on structure and antioxidant activity of whey protein isolates[J]. Food and Fermentation Industries, 2020, 46(4): 72-77.
[5]	庞鑫慧, 苏丹, 杨杨, 等. 汉麻蛋白改性及其表征方法的研究进展[J]. 食品研究与开发,2022,43(4):203−208. [PANG Xinhui, SU Dan, YANG Yang, et al. Research progress in hemp protein modification and its characterization methods[J]. Food Research and Development,2022,43(4):203−208. PANG Xinhui, SU Dan, YANG Yang, et al. Research progress in hemp protein modification and its characterization methods[J]. Food Research and Development, 2022, 43(4): 203-208.
[6]	徐瑶. 超高压辅助酶解对沙棘汁品质的影响[J]. 农产品加工,2019(23):43−45. [XU Yao. Effect of ultra high pressure enzymolysis on the quality of seabuckthorn juice[J]. Farm Products Processing,2019(23):43−45. XU Yao. Effect of ultra high pressure enzymolysis on the quality of seabuckthorn juice[J]. Farm Products Processing, 2019(23): 43-45.
[7]	郑环宇, 孙美馨, 张林, 等. 超高压均质处理大豆分离蛋白-磷脂复合物的理化性质研究[J]. 大豆科技,2019(3):14−21. [ZHENG Huanyu, SUN Meixin, ZHANG Lin, et al. Physicochemical properties of soy protein isolate-phospholipid complex treated by ultrahigh pressure homogenization[J]. Soybean Science & Technology,2019(3):14−21. ZHENG Huanyu, SUN Meixin, ZHANG Lin, et al. Physicochemical properties of soy protein isolate-phospholipid complex treated by ultrahigh pressure homogenization[J]. Soybean Science & Technology, 2019(3): 14-21.
[8]	ZHAO G, LIU Y, REN J, et al. Effect of protease pretreatment on the functional properties of protein concentrate from defatted peanut flour[J]. Journal of Food Process Engineering,2013,36(1):9−17. doi: 10.1111/j.1745-4530.2011.00646.x
[9]	WOUTERS A G B, ROMBOUTS I, MARIE L, et al. Air-water interfacial properties of enzymatic wheat gluten hydrolyzates determine their foaming behavior[J]. Food Hydrocolloids,2016,55:155−162. doi: 10.1016/j.foodhyd.2015.11.017
[10]	王章存, 袁路阳, 张露, 等. 生物酶解法去除大豆蛋白抗原性研究进展[J]. 食品工业科技,2018,39(15):317−321. [WANG Zhangcun, YUAN Luyang, ZHANG Lu, et al. Research progress of enzymatic removal of soybean protein antigenicity[J]. Science and Technology of Food Industry,2018,39(15):317−321. WANG Zhangcun, YUAN Luyang, ZHANG Lu, et al. Research progress of enzymatic removal of soybean protein antigenicity[J]. Science and Technology of Food Industry, 2018, 39(15): 317-321.
[11]	刘宁, 杨柳怡, 齐雅墨, 等. 限制性酶解-超高压处理对米渣蛋白乳化性的影响[J]. 陕西科技大学学报,2020,38(6):30−33, 39. [LIU Ning, YANG Liuyi, QI Yamo, et al. Effect of controlled enzymolysis and high hydrostatic pressure on the emulsibility of rice residue protein[J]. Journal of Shaanxi University of Science & Technology,2020,38(6):30−33, 39. LIU Ning, YANG Liuyi, QI Yamo, et al. Effect of controlled enzymolysis and high hydrostatic pressure on the emulsibility of rice residue protein[J]. Journal of Shaanxi University of Science & Technology, 2020, 38(6): 30-33, 39.
[12]	关海宁, 徐筱君, 孙薇婷, 等. 超高压协同酶法条件下不同分子量大豆分离蛋白水解肽乳化性及抗氧化性研究[J]. 食品安全质量检测学报,2022,13(12):3780−3786. [GUAN Hanning, XU Youjun, SUN Weiting, et al. Study on emulsification and antioxidant properties of soy protein isolate hydrolysatic peptides with different molecular weight by enzymatic hydrolysis under high hydrostatic pressure[J]. Journal of Food Safety & Quality,2022,13(12):3780−3786. doi: 10.3969/j.issn.2095-0381.2022.12.spaqzljcjs202212006 GUAN Hanning, XU Youjun, SUN Weiting, et al. Study on emulsification and antioxidant properties of soy protein isolate hydrolysatic peptides with different molecular weight by enzymatic hydrolysis under high hydrostatic pressure[J]. Journal of Food Safety & Quality, 2022, 13(12): 3780-3786. doi: 10.3969/j.issn.2095-0381.2022.12.spaqzljcjs202212006
[13]	杨文博, 张英华. 蛋白质水解度的测定方法研究[J]. 中国调味品,2014,39(3):88−90. [YANG Wenbo, ZHANG Yinghua. Study on the determination methods of hydrolysis degree of protein[J]. China Condiment,2014,39(3):88−90. YANG Wenbo, ZHANG Yinghua. Study on the determination methods of hydrolysis degree of protein[J]. China Condiment , 2014, 39(3): 88-90.
[14]	齐奇, 刘河涛, 杨文君, 等. 大豆胨制备工艺条件研究[J]. 粮油食品科技,2015,23(1):74−78. [QI Qi, LIU Hetao, YANG Wenjun, et al. Study on the preparation of soya peptone[J]. Science and Technology of Cereals, Oils and Foods,2015,23(1):74−78. doi: 10.3969/j.issn.1007-7561.2015.01.019 QI Qi, LIU Hetao, YANG Wenjun, et al. Study on the preparation of soya peptone[J]. Science and Technology of Cereals, Oils and Foods, 2015, 23(1): 74-78. doi: 10.3969/j.issn.1007-7561.2015.01.019
[15]	AGYARE K K, ADDO K, XIONG Y L. Emulsifying and foaming properties of transglutaminase-treated wheat gluten hydrolysate as influenced by pH, temperature and salt[J]. Food Hydrocolloids,2009,23(1):72−81. doi: 10.1016/j.foodhyd.2007.11.012
[16]	JIANG S S, MUHAMMAD A H, CHENG J J, et al. Effect of heat treatment on physicochemical and emulsifying properties of polymerized whey protein concentrate and polymerized whey protein isolate[J]. LWT,2018,98:134−140. doi: 10.1016/j.lwt.2018.08.028
[17]	WANG W, SHEN M, LIU S, et al. Gel properties and interactions of mesona blumes polysaccharidesoy protein isolates mixed gel: The effect of salt addition[J]. Carbohydrate Polymers,2018,192:193−201. doi: 10.1016/j.carbpol.2018.03.064
[18]	CHEN C, CHI Y J, ZHAO M Y, et al. Influence of degree of hydrolysis on functional properties, antioxidant and ACE inhibitory activities of egg white protein hydrolysate[J]. Food Science and Biotechnology,2012,21(1):27−34. doi: 10.1007/s10068-012-0004-6
[19]	乔杨波, 韩丽娟, 王树林, 等. 不同蛋白酶酶解对蚕豆蛋白生物活性的影响[J]. 中国油脂,2020,45(7):56−60. [QIAO Y B, HAN L J, WANG S L, et al. Effect of different protease enzymatic hydrolysis on biological activity of broad bean protein[J]. China Oils and Fats,2020,45(7):56−60. QIAO Yangbo, HAN Lijuan, WANG Shulin, et al. Effect of different protease enzymatic hydrolysis on biological activity of broad bean protein[J]. China Oils and Fats, 2020, 45(7): 56-60.
[20]	沈敏江, 王文辉, 刘丽, 等. 核桃蛋白有限酶解增溶改性的工艺研究[J]. 中国粮油学报,2015,30(8):93−98. [SHEN M J, WANG W H, LIU L, et al. Study on the technology of limited enzymatic hydrolysis and solubilization of walnut protein[J]. Journal of the Chinese Cereals and Oils Association,2015,30(8):93−98. SHEN Minjiang, WANG Wenhui, LIU Li, et al. Study on the technology of limited enzymatic hydrolysis and solubilization of walnut protein[J]. Journal of the Chinese Cereals and Oils Association, 2015, 30(8): 93-98.
[21]	李明月, 杜钰, 姚晓玲, 等. 超高压处理对蛋白质功能特性的影响[J]. 食品科技,2018,43(1):50−54. [LI Mingyue, DU Yu, YAO Xiaoling, et al. Effects of ultrahigh pressure processing on protein functional properties[J]. Food Science and Technology,2018,43(1):50−54. LI Mingyue, DU Yu, YAO Xiaoling, et al. Effects of ultrahigh pressure processing on protein functional properties[J]. Food Science and Technology, 2018, 43(1): 50-54.
[22]	魏连会, 董艳, 石杰, 等. 汉麻籽抗氧化肽的制备与氨基酸序列分析[J]. 中国油脂,2022,47(4):36−40. [WEI Lianhui, DONG Yan, SHI Jie, et al. Preparation and amino acid sequence analysis of antioxidant peptides from hemp seeds[J]. China Oils and Fats,2022,47(4):36−40. WEI Lianhui, DONG Yan, SHI Jie, et al. Preparation and amino acid sequence analysis of antioxidant peptides from hemp seeds[J]. China Oils and Fats, 2022, 47(4): 36-40.
[23]	贾聪, 华欲飞, 陈业明, 等. pH和阿拉伯胶对大豆分离蛋白/大豆蛋白酶解产物乳化性质的影响[J]. 食品与发酵工业,2017,43(5):25−29. [JIA Cong, HUA Yufei, CHEN Yeming, et al. Effect of pH and acacia on emulsifying properties of soybean protein isolate/soybean protease hydrolysate[J]. Food and Fermentation Industries,2017,43(5):25−29. JIANG Cong, HUA Yufei, CHEN Yeming, et al. Effect of pH and acacia on emulsifying properties of soybean protein isolate/soybean protease hydrolysate[J]. Food and Fermentation Industries, 2017, 43(5): 25-29.
[24]	陈林, 吴克刚, 柴向华, 等. 物理预处理改善食品蛋白酶解特性的研究进展[J]. 食品与发酵工业,2013,39(10):181−186. [CHEN Lin, WU Kegang, CHAI Xianghua, et al. Progress in improving proteolytic properties of food by physical pretreatment[J]. Food and Fermentation Industries,2013,39(10):181−186. CHEN Lin, WU Kegang, CHAI Xianghua, et al. Progress in improving proteolytic properties of food by physical pretreatment[J]. Food and Fermentation Industries, 2013, 39(10): 181-186.
[25]	于栋, 高洋, 何新蕾. 酶法制备黑豆粕粉多肽的工艺研究[J]. 中国酿造,2021,40(4):143−147. [YU Dong, GAO Yang, HE Xinlei. Preparation of polypeptides from black bean meal by enzymatic method[J]. China Brewing,2021,40(4):143−147. YU Dong, GAO Yang, HE Xinlei. Preparation of polypeptides from black bean meal by enzymatic method[J]. China Brewing, 2021, 40(4): 143-147.
[26]	张晶. 均质及酶解对大米蛋白功能特性的影响及机理的初探[D]. 合肥: 安徽农业大学, 2016: 45−49. ZHANG Jing. The effects of homogeneity and enzymatic hydrolysis on functional properties of rice protein and preliminary study on its mechanism[D]. Hefei: Anhui Agricultural University, 2016: 45−49.
[27]	陈日春, 郑宝东. 鲢鱼鱼鳞胶原蛋白抗氧化活性肽提纯及结构鉴定研究进展[J]. 食品安全质量检测学报,2017,8(4):1339−1345. [CHEN Richun, ZHENG Baodong. Research progress on purification and structure identification of antioxidant activity peptides of collagen in silver carp fish[J]. Journal of Food Safety & Quality,2017,8(4):1339−1345. CHEN Richun, ZHENG Baodong. Research progress on purification and structure identification of antioxidant activity peptides of collagen in silver carp fish[J]. Journal of Food Safety & Quality, 2017, 8(4): 1339-1345.
[28]	易佳, 刘昆仑. 超微联合超声波优化提取米糠蛋白及其对米糠蛋白溶解性的影响[J]. 食品研究与开发,2022,43(19):117−123. [YI Jia, LIU Kunlun. Optimization of extraction process of rice bran protein by ultrafine grinding combined with ultrasound and effect of extraction on protein solubility[J]. Food Research and Development,2022,43(19):117−123. doi: 10.12161/j.issn.1005-6521.2022.19.015 YI Jia, LIU Kunlun. Optimization of extraction process of rice bran protein by ultrafine grinding combined with ultrasound and effect of extraction on protein solubility[J]. Food Research and Development, 2022, 43(19): 117-123. doi: 10.12161/j.issn.1005-6521.2022.19.015
[29]	张思雨, 程建军, 孙玉雪, 等. 热处理对乳清蛋白原料起泡性、理化特性的影响及关系研究[J/OL]. 食品与发酵工业: 1−10[2022-12-22]. DOI: 10.13995/j.cnki.11-1802/ts.033203. ZHANG Siyu, CHENG Jianjun, SUN Yuxue, et al. Effect of heat treatment on foaming properties of whey protein ingredients and its relationship with physicochemical and structural[J/OL]. Food and Fermentation Industries: 1−10[2022-12-22]. DOI: 10.13995/j.cnki.11-1802/ts.033203.
[30]	PATINO R J M , CONDE M J , LINARES M H, et al. Interfacial and foaming properties of enzyme-induced hydrolysis of sunflower protein isolate[J]. Food Hydrocolloids,2007,21(5−6):782−793. doi: 10.1016/j.foodhyd.2006.09.002
[31]	ROBITAILLE G, AYERS C. Effect of K-casein glycosylation on heat stability of milk[J]. Food Research International,1995,28(1):17−21. doi: 10.1016/0963-9969(95)93326-P
[32]	ORTIZ S E M, WAGNER J R . Hydrolysates of native and modified soy protein isolates: Structural characteristics, solubility and foaming properties[J]. Food Research International,2002,35(6):511−518. doi: 10.1016/S0963-9969(01)00149-1
[33]	林素丽. 超高压处理对米糠蛋白功能及结构特性的影响研究[D]. 杭州: 浙江大学, 2017: 20−80. LIN Suli. Effects of high pressure treatment on functional and structural properties of rice bran protein[D]. Hangzhou: Zhejiang University, 2017: 20−80.
[34]	崔宪, 刘容旭, 姜帆, 等. 植物乳杆菌发酵对大豆分离蛋白功能性质影响研究[J]. 食品工业科技,2016,37(3):177−180,186. [CUI Xian, LIU Rongxu, JIANG Fan, et al. Effect of fermentation by lactobacillus plantrum on functional properties of soybean protein[J]. Science and Technology of Food Industry,2016,37(3):177−180,186. CUI Xian, LIU Rongxu, JIANG Fan, et al. Effect of fermentation by lactobacillus plantrum on functional properties of soybean protein[J]. Science and Technology of Food Industry, 2016, 37(3): 177-180, 186.
[35]	管弋铦, 何苗, 熊双丽. 超高压处理对大米蛋白功能特性及结构的影响[J]. 食品工业科技,2016,37(20):104−109. [GUAN Yixian, HE Miao, XIONG Shuangli. Effects of ultra high pressure treatment on functional properties and structure of rice proteins[J]. Science and Technology of Food Industry,2016,37(20):104−109. GUAN Yixian, HE Miao, XIONG Shuangli. Effects of ultra high pressure treatment on functional properties and structure of rice proteins[J]. Science and Technology of Food Industry, 2016, 37(20): 104-109.
[36]	胡小军, 李春兰, 王标诗, 等. 超高压处理对虾滑预制菜品质特性的影响[J]. 食品工业科技,2023,44(11):88−94. [HU X J, LI C L, WANG B S, et al. Effect of ultra-high pressure on the quality properties of shrimp slips prepared dishes[J]. Science and Technology of Food Industry,2023,44(11):88−94. HU Xiaojun, LI Chunlan, WANG Biaoshi, et al. Effect of ultra-high pressure on the quality properties of shrimp slips prepared dishes[J]. Science and Technology of Food Industry, 2023, 44(11): 88-94.
[37]	RESENDIZ V J A, ULLOA J A, URIAS S J E, et al. Effect of high-intensity ultrasound on the technofunctional properties and structure of jackfruit (Artocarpus heterophyllus) seed protein isolate[J]. Ultrasonics-Sonochemistry,2017,37:436−444. doi: 10.1016/j.ultsonch.2017.01.042

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